BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0188.p (377 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 609 e-176 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 345 3e-96 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 320 1e-88 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 318 6e-88 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 196 2e-51 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 609 bits (1571), Expect = e-176, Method: Compositional matrix adjust. Identities = 305/377 (80%), Positives = 341/377 (90%) Query: 1 LLPTLNATGSESRTRLSASQSTTGAPLITKSYQVGLAIPAYELDFFGRVRSLKDAALSTY 60 LLP LN +G+ +RTR +ASQS TG P I+ SYQVGLA+PAYELDFFGRVR+LKDAAL++Y Sbjct: 106 LLPNLNLSGNAARTRTAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASY 165 Query: 61 LSTEEARQSVQISLVSQVAQAYLTERSYAEQLKLAQQTLESRQSNLGLAQKRFDVGASSA 120 LSTEEA++S QISLV+QVAQAYL ERSYAEQLKLA +TL+SRQSNL LAQKR DVGAS+A Sbjct: 166 LSTEEAQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTA 225 Query: 121 LDLRLSDTLVQSARTSVATLLRQRAQAENALTLLVGTNVKDLPAGQDLSLQNIVTDIPAG 180 LD RL+ TLV+SAR + L RQRAQAENALTLLVG + +LP Q LS + IVTDIPAG Sbjct: 226 LDFRLTQTLVESARVAKTQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPAG 285 Query: 181 VPSDLLTRRPDIRSAEQKLLAANANIGAARAAFFPRISLTGSFGSASNSLGGLFDSGSGA 240 +PSDLLT RPDIRSAEQ LLAANANIGAARAAFFPRISLTG+FGSAS++LGGLF+SGSGA Sbjct: 286 LPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGA 345 Query: 241 WSYGPQLSLPIFDFGRNSANLDVAEVRKNQAIVTYEKTIQTAFREVADALVARGTLDEQI 300 WS+GP LSLPIFDFGRNSANLD+AEVRKN+A+VTYEK IQTAFREVADALVARG+LDEQ+ Sbjct: 346 WSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQV 405 Query: 301 EAQEAFLKAQQERLNLTDLRYKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLY 360 +AQEAFL AQQERL LTDLR+KNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLY Sbjct: 406 KAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLY 465 Query: 361 RALGGGLNETTVAAAAK 377 +ALGGGLNE TV+ AAK Sbjct: 466 KALGGGLNENTVSVAAK 482 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 345 bits (886), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 195/384 (50%), Positives = 254/384 (66%), Gaps = 11/384 (2%) Query: 1 LLPTLNATGSESRTRLSASQSTTGAPLITKSYQVGLAIPAYELDFFGRVRSLKDAALSTY 60 L P++ A SE+ +R S+TG+ L ++SY L AYELD FGRVRSLK+ AL ++ Sbjct: 110 LFPSIKAATSETASRTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSF 169 Query: 61 LSTEEARQSVQISLVSQVAQAYLTERSYAEQLKLAQQTLESRQSNLGLAQKRFDVGASSA 120 LST EAR+S QISL+++VA A+LT S ++L+LAQ TLES+ S+ L Q+ F++G+SSA Sbjct: 170 LSTAEARRSTQISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSA 229 Query: 121 LDLRLSDTLVQSARTSVATLLRQRAQAENALTLLVGTNVKDLPAG--------QDLSLQN 172 L LR + T V+SAR V + Q Q NAL LLVG KDLP L+ + Sbjct: 230 LTLRQAQTSVESARVDVESYTAQVDQDRNALVLLVG---KDLPTALLPQGLPDTRLAAAS 286 Query: 173 IVTDIPAGVPSDLLTRRPDIRSAEQKLLAANANIGAARAAFFPRISLTGSFGSASNSLGG 232 + IP G+PSDLL RR DI AE+ L AANANIGAARAAF+P I+LT + G+AS L G Sbjct: 287 PLATIPPGLPSDLLQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAG 346 Query: 233 LFDSGSGAWSYGPQLSLPIFDFGRNSANLDVAEVRKNQAIVTYEKTIQTAFREVADALVA 292 LF GSG+WS+ PQ+SLPIFD G N ANLD+A V ++ ++ YEK IQTAFREV+DAL Sbjct: 347 LFKGGSGSWSFVPQISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQ 406 Query: 293 RGTLDEQIEAQEAFLKAQQERLNLTDLRYKNGIASSLDQLDAQRDLFSAQQALIQARQLR 352 R TL Q++AQEA ++A E L+ RY +G+ S L LD+QR L+SAQ+ LI + R Sbjct: 407 RNTLGRQLQAQEALVQASDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSR 466 Query: 353 LNNAIDLYRALGGGLNETTVAAAA 376 N + Y+ LGGG E T AA Sbjct: 467 WTNLVTFYKTLGGGWVERTGTPAA 490 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 320 bits (820), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 177/371 (47%), Positives = 243/371 (65%), Gaps = 3/371 (0%) Query: 3 PTLNATGSESRTRLSASQSTTGAPLITKSYQVGLAIPAYELDFFGRVRSLKDAALSTYLS 62 P+++ATG+ S +R A STTG+ ++ V + + AYELDFFGR+++L DAAL +Y Sbjct: 119 PSISATGTSSASRTPAELSTTGSAAVSHQQSVTVGVSAYELDFFGRLKNLSDAALESYQY 178 Query: 63 TEEARQSVQISLVSQVAQAYLTERSYAEQLKLAQQTLESRQSNLGLAQKRFDVGASSALD 122 E R+S ISLV++VA A+LT S + LKLAQ T S+ L+Q+ D+G S +D Sbjct: 179 QVETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVD 238 Query: 123 LRLSDTLVQSARTSVATLLRQRAQAENALTLLVGTNVKD--LPAGQDLSLQNIVTDIPAG 180 L + V+SAR VA Q Q NAL LLVG ++ LPAG +++ PAG Sbjct: 239 LASQEATVESARADVARYASQVEQDLNALRLLVGADIDAALLPAGLP-DTASVLPATPAG 297 Query: 181 VPSDLLTRRPDIRSAEQKLLAANANIGAARAAFFPRISLTGSFGSASNSLGGLFDSGSGA 240 +PS +L RRPD+ +AE L +ANA+IGAARAAFFP ISLT + G+AS +LGGLF +GSG+ Sbjct: 298 LPSQVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRTLGGLFKAGSGS 357 Query: 241 WSYGPQLSLPIFDFGRNSANLDVAEVRKNQAIVTYEKTIQTAFREVADALVARGTLDEQI 300 W++ P ++LPIF+ G A+LD A++ ++ + YEK IQTAFREVADAL R TLD+++ Sbjct: 358 WTFSPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRL 417 Query: 301 EAQEAFLKAQQERLNLTDLRYKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLY 360 +AQ A ++ L+D RY+NG+ S L L AQR L+SAQQ LI R +N I LY Sbjct: 418 DAQGKATAASEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLY 477 Query: 361 RALGGGLNETT 371 + LGGG +E T Sbjct: 478 KVLGGGWSEQT 488 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 318 bits (814), Expect = 6e-88, Method: Compositional matrix adjust. Identities = 181/366 (49%), Positives = 251/366 (68%), Gaps = 2/366 (0%) Query: 1 LLPTLNATGSESRTRLSASQSTTGAPLITKSYQVGLAIPAYELDFFGRVRSLKDAALSTY 60 L PT+ A R+R A + T PL+ +YQ GL + ++E+D +GR+RSL DAAL + Sbjct: 110 LFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQAGLGLSSWEIDLWGRLRSLDDAALESA 169 Query: 61 LSTEEARQSVQISLVSQVAQAYLTERSYAEQLKLAQQTLESRQSNLGLAQKRFDVGASSA 120 +++ R +V +SL++QVA A+L E+L++A QT+ SRQ + + +R +VGA+S Sbjct: 170 RASQATRSAVALSLMAQVADAWLELAELDERLEIANQTIASRQESFRIFSRRVEVGATSR 229 Query: 121 LDLRLSDTLVQSARTSVATLLRQRAQAENALTLLVGTNVKDLPAGQDLS-LQNIVTDIPA 179 L+L +TL+ A+ A L +QRAQ NALTLLVG+ V LP GQ + L + + Sbjct: 230 LNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLVGSPVV-LPVGQARAGLDAVFAPLQP 288 Query: 180 GVPSDLLTRRPDIRSAEQKLLAANANIGAARAAFFPRISLTGSFGSASNSLGGLFDSGSG 239 G+P+ LLTRRPD+ +AE +L+AANANIGAARAAFFP ISLTG+ G+AS L GLF GS Sbjct: 289 GLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPSISLTGNVGTASAELSGLFRDGSH 348 Query: 240 AWSYGPQLSLPIFDFGRNSANLDVAEVRKNQAIVTYEKTIQTAFREVADALVARGTLDEQ 299 AW++ P ++LPIF GR NL ++EVR++ A+ YEKT+QTAFREVADAL AR L +Q Sbjct: 349 AWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFREVADALAARQWLTQQ 408 Query: 300 IEAQEAFLKAQQERLNLTDLRYKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDL 359 +E ++ ++ Q+ER L+ LRY NG A LD LDAQRDL SAQQ L+Q R+ L++++ L Sbjct: 409 LEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDAQRDLLSAQQQLVQIRRALLSSSVAL 468 Query: 360 YRALGG 365 Y ALGG Sbjct: 469 YAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 196 bits (498), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 139/367 (37%), Positives = 203/367 (55%), Gaps = 4/367 (1%) Query: 2 LPTLNATGSESRTRLSASQSTTGAPLITKSYQVGLAIPAYELDFFGRVRSLKDAALSTYL 61 LPT+N R+R +G T+ Y+VGL ++ELDF+GR+ SL +AAL ++ Sbjct: 104 LPTVNLEAQARRSRGPLEPPESGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWM 163 Query: 62 STEEARQSVQISLVSQVAQAYLTERSYAEQLKLAQQTLESRQSNLGLAQKRFDVGASSAL 121 ++ A Q++ ISL+ QVA A+ + R E+L L + + +L L Q R D G + A Sbjct: 164 ASRAAEQALVISLIGQVAAAWTSLRELDERLHLLETLQAATAESLALMQARHDAGRADAA 223 Query: 122 DLRLSDTLVQSARTSVATLLRQRAQAENALTLLVGTNVKDLPAGQDLSLQNIVTD--IPA 179 L + + R A L + RA + L +L G + LPA + + I+ + Sbjct: 224 ALAQQRSELAQMRAEQAELQQMRASQRSTLEMLAG--LTQLPAALEPAPTTILLPFALAP 281 Query: 180 GVPSDLLTRRPDIRSAEQKLLAANANIGAARAAFFPRISLTGSFGSASNSLGGLFDSGSG 239 G+PS LL RPDI +AE +L A+ A + AARAAFFPRISLT G AS +L LF S Sbjct: 282 GLPSTLLAARPDIIAAEHRLQASAAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSR 341 Query: 240 AWSYGPQLSLPIFDFGRNSANLDVAEVRKNQAIVTYEKTIQTAFREVADALVARGTLDEQ 299 AW++ P++SLP+ D G + D+A + + +A++ YE+T+Q AFREVA L L EQ Sbjct: 342 AWNFVPEISLPLLDGGLRQSRYDIAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQ 401 Query: 300 IEAQEAFLKAQQERLNLTDLRYKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDL 359 + + ++ Q +R +R + G AS LD L+++RD AQQ L N I L Sbjct: 402 LRLRREVVQQQAQRQAQMHMRLRYGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIAL 461 Query: 360 YRALGGG 366 Y ALGGG Sbjct: 462 YVALGGG 468 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.128 0.338 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 931,644 Number of extensions: 33163 Number of successful extensions: 202 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 172 Number of HSP's successfully gapped: 17 Length of query: 377 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 290 Effective length of database: 1,207,925 Effective search space: 350298250 Effective search space used: 350298250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)