BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0187.p (659 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum... 778 0.0 gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspiri... 448 e-127 gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspiri... 441 e-125 gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspiri... 440 e-124 >gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum seropedicae SmR1] Length = 656 Score = 778 bits (2008), Expect = 0.0, Method: Compositional matrix adjust. Identities = 402/639 (62%), Positives = 492/639 (76%), Gaps = 3/639 (0%) Query: 19 AHPLHNHPIYRPDIDGMRALAVVSVVLFHAFPDLLRGGFVGVDIFFVISGYLISGILFND 78 A P HP YRPDIDG+RA+AV+SVV+FHAFP+ L GGFVGVDIFFVISG+LISGILF+ Sbjct: 13 ASPATAHPGYRPDIDGLRAIAVLSVVVFHAFPEWLPGGFVGVDIFFVISGFLISGILFDA 72 Query: 79 LKRGSFSILDFYVRRIKRIFPALILVMLTCFVLGWFSLLAEEFRQLGKHMTAGAGFVSNI 138 L+R FSI+DFY+RRI+RIFPAL+LVML C + GWF LL EE+RQLGKH+ GAGFVSNI Sbjct: 73 LQRDRFSIVDFYIRRIRRIFPALLLVMLACLLAGWFILLPEEYRQLGKHLLGGAGFVSNI 132 Query: 139 VLWSEAGYFDTSADTKPLLHLWSLGVEEQFYLIWPLLLWAVWKRRCGVVFIVIAL--LSF 196 VLW+EAGYFDTSA+TKPLLHLWSLGVEEQFYL WPL+LWA W+RR G++ +++ + LSF Sbjct: 133 VLWNEAGYFDTSAETKPLLHLWSLGVEEQFYLFWPLMLWAAWRRRTGMLAVLLGVMALSF 192 Query: 197 GVNIATVQTDSAAAFYLPFSRFWELTAGALLAYLSFRKPGFVLPGATVFSWVGLVAVAAA 256 G NI + D+ AAFYLP SRFWEL AGALLA+ + A S GL+ + A Sbjct: 193 GWNIVWLHRDAVAAFYLPMSRFWELGAGALLAWRHRQGAVPTSVQAHRLSVAGLLLIVVA 252 Query: 257 MGLVTKTHAFPGWWAIFPVLGAWLILASGPQAWLNRRVFSARAMVWIGLISYPLYLWHWP 316 + +TK AFPGWWA+ PV GA L+LA+GPQA +NR + S R MVW+GLISYPLYLWHWP Sbjct: 253 IATITKDDAFPGWWALLPVTGAVLLLAAGPQARINRSLLSHRTMVWVGLISYPLYLWHWP 312 Query: 317 LLALARLLVGQTPPATIRIAAVCLAIALAWLTYRFVERPIRVGARGRRIAGVLACLMLFV 376 LLA ARL+VG TP +R+AAV LA+ LAWLTYR VE P+R+ + G R A LA LM + Sbjct: 313 LLAFARLVVGATPSVQLRLAAVALALLLAWLTYRLVELPVRLRSAGHRSAVTLALLMALM 372 Query: 377 GYVGYYTVQRNGMEFRDMGRITKLFNADVEQVAVLNRFELPHPSCDQLVGAAHPRDWCTN 436 +G Y V+RNGM+FR +G + LFN V++ AVLN FELPHPSC L+G H RDWC Sbjct: 373 AALGGYVVKRNGMDFRQLGAVAMLFNDQVKETAVLNHFELPHPSCAALMGQEHSRDWCAA 432 Query: 437 AVAPSQEPAFLLIGDSYSGAYAPTFASLYRSKPVPDQVFRQFARGACPSLLEYGLDYCRE 496 V+ ++ P LLIGDSYSGAYAP A LY++ P P VF+QFARG C SLL+ G YC E Sbjct: 433 PVS-AEPPQVLLIGDSYSGAYAPMLARLYQASPQPQLVFQQFARGGCASLLDGGGGYCGE 491 Query: 497 ISGKIAEYVKRTASIKTIVLAENWPGYYLADGEQFTQALERTVVFYRQLGKRVVILLSPP 556 IS ++A Y ++T SIKT+VLA NWPG+ A+ + +ALE T+++YRQLGKRVV+LLSPP Sbjct: 492 ISERVAAYARQTPSIKTVVLAANWPGHIAANPKGVLKALEHTILYYRQLGKRVVVLLSPP 551 Query: 557 NGANPKACIMRAVRLSDANFCDLPLARAEQFDTHYRDTLLPQMQRLKVPVFDPYPYFCSK 616 NGANPK+C++R +RLSDA+FC+LP A+AEQ D HYRD +LP +QRL VPVFDPYP C + Sbjct: 552 NGANPKSCVLRGIRLSDADFCNLPRAKAEQMDDHYRDLMLPLLQRLDVPVFDPYPTLCDE 611 Query: 617 DGCKVIDGPRILYYDPGHLSAYGAEFLATRASEELSALI 655 GCKVIDGPRILY+DPGHLSAYGA++LA A + L L+ Sbjct: 612 HGCKVIDGPRILYFDPGHLSAYGAQYLADHAGDALRRLL 650 >gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 662 Score = 448 bits (1152), Expect = e-127, Method: Compositional matrix adjust. Identities = 270/643 (41%), Positives = 366/643 (56%), Gaps = 35/643 (5%) Query: 25 HPIYRPDIDGMRALAVVSVVLFHAFPDLLRGGFVGVDIFFVISGYLISGILFNDLKRGSF 84 HP YRPDIDG+RA+AV SVV+FHAFP+ GGF+GVDIFFVISG+LIS ILF +L+ G F Sbjct: 16 HPAYRPDIDGLRAVAVGSVVIFHAFPEWFAGGFIGVDIFFVISGFLISLILFKNLEHGRF 75 Query: 85 SILDFYVRRIKRIFPALILVMLTCFVLGWFSLLAEEFRQLGKHMTAGAGFVSNIVLWSEA 144 SI+DFYVRR++RIFPAL+ VM C V GWF L A+E++QLGKH+ G+ F+SN + W E+ Sbjct: 76 SIVDFYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLFGGSTFISNFLFWRES 135 Query: 145 GYFDTSADTKPLLHLWSLGVEEQFYLIWPLLLWAVWKRRCGVVFI--VIALLSFGVNIAT 202 GYFD SA+TKPLLHLWSL +EEQFYL WPLLL VWKRR G + I VI LLSFGVN+ Sbjct: 136 GYFDNSAETKPLLHLWSLAIEEQFYLFWPLLLAFVWKRRWGFLGIMGVIGLLSFGVNLYF 195 Query: 203 VQTDSAAAFYLPFSRFWELTAGALLAYLSFRKPGFVLPGATVFSWVGLVAVAAAMGLVTK 262 + D +AAFY P +RFWEL G LAY++ + + +W+G + A L+ Sbjct: 196 IGHDQSAAFYSPLARFWELMIGGALAYINLHRQDLTRGYKNLQAWLGAALLVAGFALLDG 255 Query: 263 THAFPGWWAIFPVLGAWLILASGPQAWLNRRVFSARAMVWIGLISYPLYLWHWPLLALAR 322 AFPGWWA+ P LGA+L+L++GP AW NRRV +++ MV +GL+SYPLYLWHWPLLA A Sbjct: 256 ARAFPGWWALLPSLGAFLLLSAGPGAWFNRRVLASKGMVALGLVSYPLYLWHWPLLAFAT 315 Query: 323 LLVGQTPPATIRIAAVCLAIALAWLTYRFVERPIRVGARGRRIAGVLACLMLFVGYVGYY 382 ++ G++ PA R AAV A+ L+WLTYR++ERP+R R R + VL + + VG G Sbjct: 316 IIEGESAPAEWRAAAVLGALLLSWLTYRYIERPLRGSTRSRTVL-VLLAINVAVGLGGLA 374 Query: 383 TVQRNGMEFRDM-GRITKLFNADVEQVAVLNRFELPHPSCDQLVGAAHPRDWCTNAVAPS 441 + G + R + G++ L +DV + + SC + G + ++ S Sbjct: 375 IYLKGGFDERAINGKLDYLTQSDVFEGS-----RNSDGSCARFNGMEGVDE--EVCLSNS 427 Query: 442 QEPAFLLIGDSYSGAYAPTFASLYRSKP---VPDQVFRQFAR-GACPSLLEYGLDYCREI 497 P L +GDS+ A S P V R + P+ L + C I Sbjct: 428 AAPRTLFVGDSHVMALHSAIFSGDVKMPAMMVAGHACRVYPNLPYTPTHLLRWANNCTAI 487 Query: 498 SGKIAEYVKRTASIKTIVLAENWPGYYLADGEQFTQ---ALERTVVF----------YRQ 544 + + + SI T+V++ +P L+ ++ Q AL + F +R+ Sbjct: 488 ANEAIAVAGKLPSIDTVVISGAYPRGDLSQPSEYQQDGRALSKREAFLSGYGALIEAFRK 547 Query: 545 LGKRVVILLSPPN-GANPKACIMRAVRLSDANFCDLPLARAE-QFDTHYRDTLLPQMQRL 602 GK+VV + P +P AC+ R SD D AE Q D + +QR Sbjct: 548 QGKKVVFMPDVPYLKLDPHACVN---RFSDGQARDCAYTEAEHQALRRDYDAAVAALQRA 604 Query: 603 --KVPVFDPYPYFCSKDGCKVIDGPRILYYDPGHLSAYGAEFL 643 FDP FC C+ G LY D H+S + L Sbjct: 605 HPDAVFFDPQGLFCPDGKCQAKLGEAYLYNDNQHISLAASRLL 647 >gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 680 Score = 441 bits (1135), Expect = e-125, Method: Compositional matrix adjust. Identities = 269/690 (38%), Positives = 378/690 (54%), Gaps = 70/690 (10%) Query: 18 VAHPLHNHPIYRPDIDGMRALAVVSVVLFHAFPDLLRGGFVGVDIFFVISGYLISGILFN 77 + P +H YR DIDG+RA+AV++VV FH FP+ +RGGF+GVD+FFVISGYLIS I+F Sbjct: 4 LQRPALSHSKYRSDIDGLRAIAVLAVVSFHGFPNFVRGGFIGVDVFFVISGYLISTIIFE 63 Query: 78 DLKRGSFSILDFYVRRIKRIFPALILVMLTCFVLGWFSLLAEEFRQLGKHMTAGAGFVSN 137 +L RG+F+ +FY RR++RIFPAL+LV+ TCFV+GWF LL +E++QLGKH+ GAGFVSN Sbjct: 64 NLDRGTFTFTEFYARRVRRIFPALLLVLSTCFVVGWFVLLTDEYKQLGKHIAGGAGFVSN 123 Query: 138 IVLWSEAGYFDTSADTKPLLHLWSLGVEEQFYLIWPLLLWAVWKRRCGV--VFIVIALLS 195 +L E+GYFD SA+TKPLLHLWSLG+EEQFY++WP+LL+ W+R+ + + I++AL S Sbjct: 124 FLLRDESGYFDNSAETKPLLHLWSLGIEEQFYILWPMLLFFSWRRKFNLLTITIIVALAS 183 Query: 196 FGVNIATVQTDSAAAFYLPFSRFWELTAGALLAYLSFRKPGFVLP-----GATVFSWVG- 249 F NI + D+ AAFYLP SRFWEL +G LLA+ + K + G + + Sbjct: 184 FAANIKGINKDATAAFYLPQSRFWELLSGTLLAWFTLYKQHTTIELRPRLGTWILQLIYS 243 Query: 250 ---------LVAVAAAMGL---------VTKTHAFPGWWAIFPVLGAWLILASGPQAWLN 291 L+ V++ GL + K AFPG WA+ P+LG LI+A+GP AW+N Sbjct: 244 EKRKSDGKFLIDVSSITGLCIVLYGFWRINKELAFPGVWALVPILGTILIIAAGPAAWVN 303 Query: 292 RRVFSARAMVWIGLISYPLYLWHWPLLALARLLVGQTPPATIRIAAVCLAIALAWLTYRF 351 R + S + +VW GLIS+PLYLWHWP+L+ AR++ + P +R+ A+ LAI LAWLTY Sbjct: 304 RTLLSNKILVWFGLISFPLYLWHWPVLSFARIVENELPSYPLRVGAIALAIILAWLTYEI 363 Query: 352 VERPIRVG--ARGRRIAGVLACLMLFVGYVGYYTVQRNGMEFRDMGRITKLFNADVEQVA 409 VERPIR G A + L LM VG VGY T NG E R IT++ A Sbjct: 364 VERPIRTGNNANTKITVTALIGLMALVGAVGYRTFAANGWESRMSPDITRISRAAG---- 419 Query: 410 VLNRFELPHPSCDQLVGAAHPRDWCTNAVAPSQEPAFLLIGDSYSGAYAPTFASLYRSKP 469 E +P + + N+ + + L IGDS Y L +KP Sbjct: 420 -----EWQYPGKLTAFNFENRTFYRQNS---NMDEITLFIGDSNIEQYYVRTDELISTKP 471 Query: 470 VPDQVFRQFARGACPSLLEYGLDY----CREISGKIAEYVKRTASIKTIVLAENWPGYYL 525 G C ++ D C + Y K AS+ ++V+ W YL Sbjct: 472 NNSNSVVFATGGGCLAIPGSPYDEAHMSCANLMETALNYAKTNASVASVVIGAQWNS-YL 530 Query: 526 AD-----------GEQFTQALERTVVFYRQ---LGKRVVILLSPPNGA--NPKACIMRAV 569 +D G+ ++ AL R + R+ LGK+V +L+ P G +PK I R + Sbjct: 531 SDGYGLRGSFGTAGKDYSAALNRLARYIRELKYLGKKVYFILNIPIGDELDPKFMITRNL 590 Query: 570 RL--SDANFCDLPLARA---EQFDTHYRDTLLPQMQRLK-VPVFDPYPYFCSKDGCKVID 623 + N + L R E++ +D L Q+ R V +P + C+ C +D Sbjct: 591 KHFPHVINLKERGLNRKDIDERYGKIQKD--LEQIARAAGADVVNPMDFLCNATKCSSLD 648 Query: 624 GP-RILYYDPGHLSAYGAEFLATRASEELS 652 +Y D HL + A+ E L+ Sbjct: 649 DEGEPIYKDRSHLRPQYVRYRASFIDEFLT 678 >gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 681 Score = 440 bits (1132), Expect = e-124, Method: Compositional matrix adjust. Identities = 269/641 (41%), Positives = 365/641 (56%), Gaps = 44/641 (6%) Query: 28 YRPDIDGMRALAVVSVVLFHAFPDLLRGGFVGVDIFFVISGYLISGILFNDLKRGSFSIL 87 YRPDIDG+RA+A+VSVV+FHAFPD + GGF+GVDIFFVISG+LIS I+F L +G FS Sbjct: 32 YRPDIDGLRAIAIVSVVVFHAFPDAMPGGFIGVDIFFVISGFLISSIIFRQLDQGRFSFT 91 Query: 88 DFYVRRIKRIFPALILVMLTCFVLGWFSLLAEEFRQLGKHMTAGAGFVSNIVLWSEAGYF 147 DFYV RI+RIFPALILV+ +CFVLGWFSLL EE+RQLGKHM AGAGF+ N+VLW E+GYF Sbjct: 92 DFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGAGFIQNLVLWKESGYF 151 Query: 148 DTSADTKPLLHLWSLGVEEQFYLIWPLLLWAVWKRRCGV--VFIVIALLSFGVNIATVQT 205 D +D KPLLHLWSL VEEQFYL++PLL+ +W+ R + +++ L SF +N+ V Sbjct: 152 DVESDMKPLLHLWSLSVEEQFYLVYPLLVCGLWRARMPLRNSLVLLMLASFALNLWWVAQ 211 Query: 206 DSAAAFYLPFSRFWELTAGALLAY-----------LSFRKPGFVLPGATVFSWVGLVAVA 254 D FYLPF+R WEL AGAL A+ SFR P LP + S +GL + Sbjct: 212 DPVKTFYLPFTRVWELLAGALCAFGVMRKGVAFSTSSFRLPH--LPSIHLRSVLGLALLL 269 Query: 255 AAMGLVTKTHAFPGWWAIFPVLGAWLILASGPQAWLNRRVFSARAMVWIGLISYPLYLWH 314 + L+ + FPG WA+ PV GA L+++SGPQAW+NR + S +VWIGL+SY LYLWH Sbjct: 270 CGLVLIDRHRYFPGAWALLPVTGAVLLVSSGPQAWVNRHLLSRPLLVWIGLVSYLLYLWH 329 Query: 315 WPLLALARLLVGQTPPATIRIAAVCLAIALAWLTYRFVERPIRVGARGRRIAGVLACLML 374 WP+L+ +L P A + +AAV L++ LAWLTYR +ERP+R GA+ + +LA L+ Sbjct: 330 WPILSFMHILTPTRPGAPLPLAAVALSVLLAWLTYRLLERPVRFGAKSWMVPALLALLLS 389 Query: 375 FVGYVGYYTVQRNGMEFRDMGRITKLFNADVEQVAVLNRFELPHPSCDQLVGAAHPRDWC 434 GY QRNG+ FR L EQ + + +C GA ++C Sbjct: 390 VTATAGYNDFQRNGLSFR-------LATHYAEQPLFVGTTD---ATCTADGGAG--LEFC 437 Query: 435 TNAVAPSQEPAFLLIGDSYSGAYAPTFASLYRSKPVPDQVFRQFARGACPSLLEYGLDYC 494 + + P ++IGDS++ AS + A GAC D Sbjct: 438 RQSRPGA--PTIVVIGDSHAWHLYDGLASYAAQN---GDIIGMRAVGACLGFSGVSADTK 492 Query: 495 REISGKIAEYVK---RTASIKTIVLAENWPGYYLA-DGEQFTQALERTVVFYRQLGKRVV 550 R + + R AS++T+VL+ Y GE F AL + GK+V+ Sbjct: 493 RHDCAPARDSIASAARQASVRTVVLSARLDIYDRELPGEVFEGALRDMLATLTAAGKKVI 552 Query: 551 ILLSPPN-GANPKACI-----MRAVRLSDANFCDLPLARAEQFDTHYRDTLLPQMQRL-K 603 ++ P +PK C+ +R S N C + A Q HYR+T+ +++ + Sbjct: 553 FVMDIPILDFHPKYCLDISMPLRPKTTSLPN-CAIQQAVFAQHTQHYRETVNALLRQFPQ 611 Query: 604 VPVFDPYPYFCSKDGCKVIDGPRILYYDPGHLSAYGAEFLA 644 V FD C C + ++LY D HLS G+ +A Sbjct: 612 VQAFDAAAPLCDHQQCWAMKDGQLLYRDYDHLSTIGSTLVA 652 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.463 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,141,110 Number of extensions: 91560 Number of successful extensions: 340 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 327 Number of HSP's successfully gapped: 5 Length of query: 659 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 568 Effective length of database: 1,188,985 Effective search space: 675343480 Effective search space used: 675343480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 134 (56.2 bits)