BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0176.p (336 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313504|ref|YP_003777596.1| galactose-1-phosphate uridylylt... 531 e-152 gi|300313507|ref|YP_003777599.1| polysaccharide biosynthesis pro... 150 1e-37 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 71 1e-13 gi|300313691|ref|YP_003777783.1| CDP-glucose-4,6-dehydratase [He... 59 4e-10 >gi|300313504|ref|YP_003777596.1| galactose-1-phosphate uridylyltransferase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 531 bits (1368), Expect = e-152, Method: Compositional matrix adjust. Identities = 255/336 (75%), Positives = 289/336 (86%) Query: 1 MFDNKVLMITGGTGSFGNTVLKRFLDTAVREIRIFSRDEKKQEDMRIALKNDKVKFYIGD 60 MFD+ VLMITGGTGSFG TVL RFL+T VREIR+FSRDEKKQED+RIAL N+K++FYIGD Sbjct: 1 MFDDSVLMITGGTGSFGQTVLNRFLNTGVREIRVFSRDEKKQEDLRIALNNEKLRFYIGD 60 Query: 61 VRNYDSIAQAMVGVDFVFHAAALKQVPSCEFYPMEAIRTNILGTENVLNAAIASKVKRVV 120 VR+ DS+ AM GVD+VFHAAALKQVPSCEFYPMEA++TN++GTENVL + V+RVV Sbjct: 61 VRDPDSLRAAMKGVDYVFHAAALKQVPSCEFYPMEAVKTNVIGTENVLRVSSECGVRRVV 120 Query: 121 VLSTDKAVYPINAMGISKAMAEKLVTAKSRGIPADGPRICATRYGNVMASRGSVIPLFVS 180 VLSTDKAVYPINAMGISKAMAEK++ A +R CATRYGNVMASRGSVIPLFVS Sbjct: 121 VLSTDKAVYPINAMGISKAMAEKVMVAAARTQRQGETVFCATRYGNVMASRGSVIPLFVS 180 Query: 181 QLKAGQPLTVTDPNMTRFLMSLEDSVDLVLHAFEHAQQGDLFVQKAPASTVADLAQALRE 240 Q+KA +P+T+TDPNMTRFLMSLEDSVDLVL+AF+H QQGD+FVQKAPASTVADLA AL+E Sbjct: 181 QIKAEKPITITDPNMTRFLMSLEDSVDLVLYAFQHGQQGDIFVQKAPASTVADLASALKE 240 Query: 241 LFKLDNPVKVIGTRHGEKLYESLISREEMAKAEDMGRYYRVPADNRDLNYNKYFVEGESH 300 LF D VK IGTRHGEKLYESLISREEMA A+DMG YYR+PADNRDLNY KYF EG+ Sbjct: 241 LFSSDTVVKTIGTRHGEKLYESLISREEMAHAQDMGDYYRIPADNRDLNYAKYFSEGDQD 300 Query: 301 ISELDDYTSHNTQRLNVEEIKTLLLKLPEIREALDA 336 IS DDYTSHNT+RLNVE+IK LLL L I+E L+A Sbjct: 301 ISLFDDYTSHNTRRLNVEQIKQLLLGLDFIKEELNA 336 >gi|300313507|ref|YP_003777599.1| polysaccharide biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 648 Score = 150 bits (380), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 97/280 (34%), Positives = 155/280 (55%), Gaps = 21/280 (7%) Query: 4 NKVLMITGGTGSFGNTVLKRFLDTAVREIRIFSRDE------KKQEDMRIALKNDKVKFY 57 +KV+M+TG GS G+ + ++ L ++ + + E +Q + ++A ++ + Sbjct: 290 SKVVMVTGAGGSIGSELCRQILAVGPSKLLLIEQSEFALYAIHQQLEEKLADRDIVLVPL 349 Query: 58 IGDVRNYDSIAQAMVGV--DFVFHAAALKQVPSCEFYPMEAIRTNILGTENVLNAAIASK 115 + V++ + I + M D V+HAAA K VP E P E I+ N+LGT AA + Sbjct: 350 LASVQDSERIREIMSTWHPDTVYHAAAYKHVPLVEHNPAEGIKNNVLGTLRTAQAATENG 409 Query: 116 VKRVVVLSTDKAVYPINAMGISKAMAEKLVTAKSRGIPADGPRICATRYGNVMASRGSVI 175 V V++STDKAV P N MG SK +AE ++ A + P G + R+GNV+ S GSV+ Sbjct: 410 VADFVLISTDKAVRPTNIMGASKRLAEMVLQAIAATSP--GTKFSMVRFGNVLGSSGSVV 467 Query: 176 PLFVSQLKAGQPLTVTDPNMTRFLMSLEDSVDLVLHAFEHAQQGDLFV-QKAPASTVADL 234 P F Q++ G P+T+T ++TR+ M++ ++ LV+ A A+ GD+FV A + DL Sbjct: 468 PKFRQQIRDGGPITLTHADITRYFMTIPEASQLVIQAGAMAKGGDVFVLDMGQAVRIMDL 527 Query: 235 AQALRELFKLD-----NP-----VKVIGTRHGEKLYESLI 264 A+ + EL L NP +++ G R GEKLYE L+ Sbjct: 528 ARRMIELSGLTVKDELNPEGDIAIEITGLRPGEKLYEELL 567 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 70.9 bits (172), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 46/129 (35%), Positives = 71/129 (55%), Gaps = 5/129 (3%) Query: 4 NKVLMITGGTGSFGNTVLKRFLDTAVREIRIFSRDEK-KQEDMRIALKNDKVKFY--IGD 60 K +++ GG G G+ + + V+EI I+ + +E+++ ALK+ +VK Y GD Sbjct: 5 GKKIVVIGGAGLIGSHTVDHLVKEDVKEILIYDNFLRGSRENLQNALKDPRVKIYDVGGD 64 Query: 61 VRNYDSIAQAMVGVDFVFHAAALKQVPSCEFYPMEAIRTNILGTENVLNAAIASKVKRVV 120 + D + A+ G D VFH AAL + C YP A N+ GT NV+ A +A VKR + Sbjct: 65 IMQTDILQSALEGADGVFHLAAL-WLLQCHEYPRSAFDVNVRGTFNVMEACVAKGVKR-L 122 Query: 121 VLSTDKAVY 129 V S+ +VY Sbjct: 123 VYSSSASVY 131 >gi|300313691|ref|YP_003777783.1| CDP-glucose-4,6-dehydratase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 59.3 bits (142), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 57/224 (25%), Positives = 98/224 (43%), Gaps = 28/224 (12%) Query: 1 MFDNKVLMITGGTGSFGNTVLKRFLDTAVREIRIFSRDEKKQEDM-RIALKNDKVKFYIG 59 + +K + ITG TG F + L +L ++ +S + +A ++ V+ G Sbjct: 4 FWRDKRVFITGHTG-FKGSWLTLWLHLMGAKVSGYSLPAPTSPSLFHLARLHNCVQTTTG 62 Query: 60 DVRNYDSIAQAMVGV--DFVFHAAALKQVPSCEFYPMEAIRTNILGTENVLNAAIASKVK 117 DVR+ +A+ M V D VFH A Q+ P++ TN++GT ++L A ++ Sbjct: 63 DVRDPKHLAETMAAVQPDVVFHLAGQPQIEHSYLTPLDTFATNVMGTAHLLEAVRSTPSV 122 Query: 118 RVVVLSTDKAVY-------------PI---NAMGISKAMAEKLVTAKSRGIPADGPR--- 158 R VV+ + Y P+ + SK AE + A R + P Sbjct: 123 RAVVVVSSNKCYENREWLWGYREDDPMGGHDPFSSSKGAAELVTAAYRRSFFSADPDSPV 182 Query: 159 -ICATRYGNVMA----SRGSVIPLFVSQLKAGQPLTVTDPNMTR 197 + + R GNV+ + +IP F+ L+ QP+T+ P+ R Sbjct: 183 GVASVRTGNVIGGGDFAADRLIPDFIRALETDQPVTIRQPHAVR 226 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 937,918 Number of extensions: 35398 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 4 Length of query: 336 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 250 Effective length of database: 1,212,660 Effective search space: 303165000 Effective search space used: 303165000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)