BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0175.p (288 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313503|ref|YP_003777595.1| dTDP-4-dehydrorhamnose reductas... 280 1e-76 gi|300313656|ref|YP_003777748.1| dTDP-4-dehydrorhamnose reductas... 58 8e-10 >gi|300313503|ref|YP_003777595.1| dTDP-4-dehydrorhamnose reductase [Herbaspirillum seropedicae SmR1] Length = 285 Score = 280 bits (715), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 147/291 (50%), Positives = 189/291 (64%), Gaps = 10/291 (3%) Query: 1 MKILVLGATGMLGNAMMRVLSEAVDFNVLGTMRSEAAKRFFAPEIASHLISGIDVADTNV 60 MK+LVLGA+GM+G M VLS+ + V +R AK F E+ + +++GID+++ + Sbjct: 1 MKVLVLGASGMIGRTMFHVLSQRPGWEVCAIVR---AKTF---EVGAPVVTGIDLSNQDH 54 Query: 61 LADIFHKHKPDVVINCIGLIKQLAQAEDPVEAITINSLLPQRLKKLCDAAHIRLIHFSTD 120 + +F + +PDVV+NC GL K L + + A+T+N+LLP RL +LCD A RLIH STD Sbjct: 55 MERLFAQRRPDVVVNCAGLTKHLPEGNAHIPALTMNALLPHRLAQLCDIADARLIHVSTD 114 Query: 121 CVFSGSRGGYSEDELPDARDLYGRSKLLGEVDGENVVTLRTSIIGHELVGAHSLIGWFLA 180 CVFSG G Y E + PDA D+YGR+K LGEV N +TLRTS IGHE L+ WFLA Sbjct: 115 CVFSGDAGNYKEQDTPDATDVYGRTKALGEVTAGNALTLRTSTIGHEHGTQFGLLEWFLA 174 Query: 181 QQGQCTGYKKAIFSGLPTPVMAAIVRDHVIPAPDLHGLYHVAAEPISKFDLLTLVADVYG 240 Q QC G++ AIFSGLPT A +VRD VIP L GLYHV A PI K LL +A VY Sbjct: 175 QT-QCKGFRHAIFSGLPTVEFARVVRDIVIPDSSLKGLYHVGASPIDKLSLLQTIARVYK 233 Query: 241 KRIDIIPDESLVINRSLSADRFAGKTGFRAAPWRKLIEAM---HSRYKASD 288 + I I DE I+RSL+AD+FA TG+RA W LIEAM H+ K S+ Sbjct: 234 RDIAITVDEEFRIDRSLNADKFAAATGYRAPAWPVLIEAMYEDHTSRKQSN 284 >gi|300313656|ref|YP_003777748.1| dTDP-4-dehydrorhamnose reductase [Herbaspirillum seropedicae SmR1] Length = 312 Score = 58.2 bits (139), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 41/151 (27%), Positives = 67/151 (44%), Gaps = 20/151 (13%) Query: 1 MKILVLGATGMLGNAMMRVLSEAVDFNVLGTMRSEAAKRFFAPEIASHLISGIDVADTNV 60 M+IL+ GA+G LG + +L+ G + + +++ +D+AD Sbjct: 1 MRILLTGASGQLGQVLQPMLARH------GELLAPSSRE-------------LDLADRQA 41 Query: 61 LADIFHKHKPDVVINCIGLIKQLAQAEDPVEAITINSLLPQRLKKLCDAAHIRLIHFSTD 120 L + +P +++N D A +N+L P L + L+HFSTD Sbjct: 42 LQSFLQQARPGLIVNAAAYTAVDKAESDVARAEAVNALAPMILAQQAQMLGAPLVHFSTD 101 Query: 121 CVFSG-SRGGYSEDELPDARDLYGRSKLLGE 150 VF G S Y E + ++YGR+KL GE Sbjct: 102 YVFDGKSARPYVETDPTGPLNVYGRTKLAGE 132 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 903,073 Number of extensions: 38377 Number of successful extensions: 115 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 111 Number of HSP's successfully gapped: 2 Length of query: 288 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 204 Effective length of database: 1,222,130 Effective search space: 249314520 Effective search space used: 249314520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)