BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0146.p (361 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [H... 75 7e-15 gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 61 1e-10 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 59 5e-10 >gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [Herbaspirillum seropedicae SmR1] Length = 338 Score = 75.1 bits (183), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 85/348 (24%), Positives = 147/348 (42%), Gaps = 41/348 (11%) Query: 6 LITGVTGQDGA-YLAELLLDKGYE-VHGIKRRTSLLNTDRIDHLYQDPHEKNVRFKLHHG 63 +TG G GA Y+ L E V I + T N D + + D R+ Sbjct: 3 FVTGGAGFIGANYILHFLAAPHDEAVLNIDKLTYAGNPDNLASVAGD-----ARYLFSQT 57 Query: 64 DLTDSTSLIRIIQLVQPDEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAI-----R 118 D+ D+ ++ R+ +P + + AA+SHV S P ++ G +L A Sbjct: 58 DICDTAAIARLFAQHRPRAVVHCAAESHVDRSIAGPAVFIQTNVNGTFSMLSAAYDYWST 117 Query: 119 ILGLEKKA-RFYQASTSELYG-LVQEMPQ-KETTPFYPRSPYAVAKLYAYWITVNYREAY 175 + E+ A RF ST E++G L + P ET+P+ P SPY+ +K + + + Y Sbjct: 118 LPAAEQAAFRFLHISTDEVFGSLAADAPPFTETSPYAPNSPYSASKAASDHLVRAFHSTY 177 Query: 176 GIYACNGILFNHESPIRGETFVTRKITRALARIKLGLQDCVYLGNLNAKRDWGHARDYVE 235 G+ +L H S G K+ + L Q G+ RDW H D+ Sbjct: 178 GL----PVLITHCSNNYGPFHFPEKLIPLVITNALAGQPLPIYGDGQQVRDWLHVADHCR 233 Query: 236 MQWLMLQQEQAEDFVIATGVQYSVRDFINAAAKEIDIEIRWE-GEGLEEKGYDASGK--- 291 L+L + + G Y+ I +++ ++E+ + L+E A G+ Sbjct: 234 AIELVLARAR-------PGESYN----IGGNSEKTNLEVVLTLCDLLDELAPKAQGQYRD 282 Query: 292 --CIVAVDPRYFRPAEVETLLGDASKAKQKLGWTPKTTFAELVQEMMR 337 C V P + R V+T SK +++LGW+P+ F + +++ ++ Sbjct: 283 QICFVQDRPGHDRRYAVDT-----SKIQRELGWSPQHAFRQGLRQTVQ 325 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 78/337 (23%), Positives = 143/337 (42%), Gaps = 50/337 (14%) Query: 6 LITGVTGQDGAYLAELLLDKGYEVHGIKR--RTSLLNTDRIDHLYQDPHEKNVRFKLHHG 63 L+TG G G++ LL G+EV +S + R+ + L G Sbjct: 4 LVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQI------TGKTLSLVKG 57 Query: 64 DLTDSTSLIRIIQLVQPDEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIRILGLE 123 D+ + L +++ + + + + A V S E+P +++ +G +R+LEA++ G++ Sbjct: 58 DIRNREQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVK 117 Query: 124 KKARFYQASTSELYGLVQEMPQKETTPFYPRSPYAVAKLYAYWITVNYREAYGIYACNGI 183 +S++ +YG Q +P E P +PY +K+ + +Y A+ + + Sbjct: 118 T---IVFSSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALL 174 Query: 184 -LFN----HESPIRGE--TFVTRKITRALARIKLGLQD--CVYLGNL-----NAKRDWGH 229 FN HES + GE + + +A++ +G ++ ++ G+ RD+ H Sbjct: 175 RYFNPVGAHESGLIGEDPGGIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIH 234 Query: 230 ARDYVEMQWLMLQQEQAEDFV---IATGVQYSVRDFINAAAKEIDIEIRWEGEGLEEKGY 286 D LQ A + TGV YSV D + A K Sbjct: 235 VVDLARGHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEK------------------ 276 Query: 287 DASGKCI-VAVDPRYFRPAEVETLLGDASKAKQKLGW 322 A GK + A+ R RP ++ + + ++AK+KLGW Sbjct: 277 -ACGKTLPYAIAER--RPGDIASCYANPAEAKRKLGW 310 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 59.3 bits (142), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 52/160 (32%), Positives = 75/160 (46%), Gaps = 13/160 (8%) Query: 6 LITGVTGQDGAYLAELLLDKGYEVHGIKRRTSLLNTDRIDHLYQDPHEKNV----RFKLH 61 LITGV G G+ L LL G +V G+ +T R +L + E F+ Sbjct: 19 LITGVAGFIGSNLLARLLTLGQQVTGLDN----FSTGRQANLEEVRAEVGAAAWANFRFI 74 Query: 62 HGDLTDSTSLIRIIQLVQPDEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIRILG 121 GD+ D+ + +R D + + AA V+ S E+P T ++ G L +L A R G Sbjct: 75 EGDICDAATCLRACD--GADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARDAG 132 Query: 122 LEKKARFYQASTSELYGLVQEMPQKETTPFYPRSPYAVAK 161 + ARF A++S YG +P+ E P SPYAV K Sbjct: 133 V---ARFVYAASSSTYGDHPGLPKVEEVIGKPLSPYAVTK 169 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,089,844 Number of extensions: 43007 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 3 Length of query: 361 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 275 Effective length of database: 1,212,660 Effective search space: 333481500 Effective search space used: 333481500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)