BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0144.p (272 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309812|ref|YP_003773904.1| bis(5'-nucleosyl)-tetraphosphat... 373 e-104 >gi|300309812|ref|YP_003773904.1| bis(5'-nucleosyl)-tetraphosphatase [Herbaspirillum seropedicae SmR1] Length = 274 Score = 373 bits (957), Expect = e-104, Method: Compositional matrix adjust. Identities = 181/269 (67%), Positives = 210/269 (78%), Gaps = 5/269 (1%) Query: 4 YAIGDLQGCNQQLQNLLDLIEKT--SPDAQLLFVGDLVNRGPQSLQTLRRLRELGSRAKA 61 Y IGDLQGC L LDL + S DAQL+F GDL+NRGPQSLQTLR + ++G RA+A Sbjct: 7 YVIGDLQGC---LTPFLDLQRQLPLSADAQLIFAGDLINRGPQSLQTLRTVMQMGQRAQA 63 Query: 62 VLGNHDLHLLAVSQGIRPQHRTDTLDDILNAPDRDELLDWLRHRPLALFEQGHLLFHAGL 121 VLGNHDLHLLAV+ GIRP HR DTL +IL APDR+EL+ WLRHRPLA+ Q HL+ HAG+ Sbjct: 64 VLGNHDLHLLAVACGIRPAHRDDTLAEILQAPDREELIHWLRHRPLAIHAQDHLIVHAGV 123 Query: 122 FPQWDAAKTMALAQEVEAMLRGPDWIDFLRHMYGNEPAVWSDELEGYDRLRCIINTLTRM 181 PQWD + +ALA EVE MLR WI FLRHMYGN+P WSD+L GYDRLRCIIN TR+ Sbjct: 124 LPQWDLQQALALAGEVETMLRSDQWIAFLRHMYGNQPDRWSDDLVGYDRLRCIINAFTRV 183 Query: 182 RFCTPDGAIDFKIKEGLGAAPDGYLPWFDMPGRQTAGVTCVFGHWASLGLTRRPNLIGLD 241 RFC DG +DF +KEG AAP G PWFD+PGR+T VT VFGHW++LGL RPNLI LD Sbjct: 184 RFCQADGTMDFAVKEGAAAAPAGLTPWFDVPGRRTEEVTVVFGHWSTLGLVMRPNLIALD 243 Query: 242 TGCVWGGKLTAVRLEDRALFQVDCPRAEQ 270 TGCVWGG+L+AVRLEDRALFQV CP+A + Sbjct: 244 TGCVWGGQLSAVRLEDRALFQVQCPQARR 272 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.141 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 893,675 Number of extensions: 39347 Number of successful extensions: 117 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 117 Number of HSP's successfully gapped: 1 Length of query: 272 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 188 Effective length of database: 1,222,130 Effective search space: 229760440 Effective search space used: 229760440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)