BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0102.p (293 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 92 4e-20 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 81 1e-16 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 80 2e-16 gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 80 3e-16 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 92.4 bits (228), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 82/278 (29%), Positives = 142/278 (51%), Gaps = 19/278 (6%) Query: 3 GITHSFSRLGNAVAPPIVALLIVLHSWRAAFIIVGALTALWIIAWAVYYKDNPREHKAIN 62 G +S + LG +APP+V L + + W++AF++ G L +W W +Y+ +PR+H A++ Sbjct: 137 GWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAALWFAFYR-SPRDHAALS 195 Query: 63 EAELARL--PEHVKVTANGGDTPWKKILRRM---SPTIFVYFCQVWTNTLFFSWVPIFFM 117 ++E + + V+A G P K ++R + + +F + T F W+P++F+ Sbjct: 196 DSERETIISGQLRPVSAVRGKRPVKDVVRSRRFWAIALARFFAEPAWQTFSF-WIPLYFV 254 Query: 118 NAYHLDIKKSAIFASGVFFAGVVGDVTGGLISDKVLKKTGSLPMARQ---GVI--AFSLL 172 N HLD+K A+FA F A +G + GG +S ++ + +P+ GV+ A ++ Sbjct: 255 NERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRF-RIPLVWSRVCGVVLGAVMMI 313 Query: 173 GTLCFLVPVMLSHDVNVVAWCLSGAFFMLELTIGPIWAVPMDV-APEYTGTASGMLNTGA 231 G C V ++ S V + +C+ G F ++ G + + DV PE GTASG A Sbjct: 314 GPAC--VGLVASPYVAIALFCVGG--FAHQMISGLVNTLSADVFDPEEVGTASGFAGMSA 369 Query: 232 ALATIISPVVFGTVIDLTGNWNLPFVGSAVFLLIGAVM 269 + + ++ G + D G L F F LIGAV+ Sbjct: 370 WIGGLGFSLLVGALADTVGYGPL-FGMLGAFDLIGAVL 406 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 80.9 bits (198), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 77/273 (28%), Positives = 138/273 (50%), Gaps = 32/273 (11%) Query: 18 PIVALLIVLHSWRAAFIIVGAL-TALWIIAWAVYYKDNPREHKAINEAELARLP------ 70 P++A ++ +SW F ++G + AL +I + Y +P++H IN+AELA + Sbjct: 162 PLMAWIVHTYSWHHVFTVMGVVGIALALIWGRIIY--SPKDHPRINQAELAHIESGGGLV 219 Query: 71 --EHVKVTA--NGGDTPWKKIL-RRMSPTIFV-YFCQVWTNTLFFSWVPIFFMNAYHLDI 124 ++ KV A G +++L RM +++ +C F +W P++ + + I Sbjct: 220 DMDNRKVAAPSTKGRGYIRQLLSNRMLLGVYIGQYCINVITYFFLTWFPVYLVQERGMSI 279 Query: 125 KKSAIFASGVFFAGVVGDVTGGLISDKVLKKTGSLPMARQGVIAFSLLGTLCFLVPVMLS 184 K+ AS G +G V GGLISD+++K+ S+ AR+ ++G + + ++L Sbjct: 280 LKAGFVASIPAVCGFIGGVVGGLISDRLIKRGLSVTWARK----IPIVGGMLLSMTMILC 335 Query: 185 HDVNVVAWCLSG----AFFMLELTIGPI-WAVPMDVAP-EYTGTASGMLNTGAALATIIS 238 + V W + G AFF +G + WAV D AP + G + G+ N +A I + Sbjct: 336 NYVE-AQWMVVGIMALAFF--GKGVGALGWAVVADTAPKQIVGLSGGLFNMFGNVAGITT 392 Query: 239 PVVFGTVIDLTGNWNLPFVGSAVFLLIGAVMAL 271 P+V G ++ TG+ F G+ VF+ I A++ + Sbjct: 393 PIVIGYIVKETGS----FSGALVFVGINALVTV 421 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 80.1 bits (196), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 60/254 (23%), Positives = 115/254 (45%), Gaps = 9/254 (3%) Query: 3 GITHSFSRLGNAVAPPIVALLIVLHSWRAAFIIVGALTALWIIAWAVYYKDNPREHKAIN 62 GI + S +G A+APPI+ +L++ WR F+I+G L + W ++Y++ R + Sbjct: 144 GIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSVGWYLFYRN--RNEVQLT 201 Query: 63 EAELARLPEHVKVTANGGD----TPWKKIL--RRMSPTIFVYFCQVWTNTLFFSWVPIFF 116 +E+ L + N + WK + R +F + ++ L+ +W+P + Sbjct: 202 PSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFMGVIYMVWLYLTWLPAYL 261 Query: 117 MNAYHLDIKKSAIFASGVFFAGVVGDVTGGLISDKVLKKTGSLPMARQGVIAFSLLGTLC 176 + HL I ++ + G +G ++ G I+D ++K+ +R+ I L+G Sbjct: 262 EHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKRGMPAIRSRKWPICAGLIGAGA 321 Query: 177 FLVPVMLSHDVNVVAWCLSGAFFMLELTIGPIWA-VPMDVAPEYTGTASGMLNTGAALAT 235 F +P + + +S A + + G WA V + + + M N G L Sbjct: 322 FTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIPRRLVASLGSMQNFGGYLGG 381 Query: 236 IISPVVFGTVIDLT 249 ++PV+ G V+D T Sbjct: 382 SLAPVITGVVVDQT 395 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 79.7 bits (195), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 72/251 (28%), Positives = 120/251 (47%), Gaps = 15/251 (5%) Query: 9 SRLGNAVAPPIVALLIVLHSWRAAFIIVGALTALWIIAWAVYYKDNPREHKAINEAELAR 68 S +G APP+V I+ W+ +F++VGAL +W + W ++YK +PR+ K ++ E A Sbjct: 143 SSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWMLFYK-SPRDQKLLSPEERAY 201 Query: 69 LPE-HVKVTANGGDTPWKKILRRM---SPTIFVYFCQ-VWTNTLFFSWVPIFFMNAYHLD 123 + E K W KI+R S I + + W F +W+P++ H++ Sbjct: 202 ILEGQEKSPEKVQRESWTKIVRSRNFWSIAIPRFLSEPAWQT--FNAWIPLYMATERHMN 259 Query: 124 IKKSAIFASGVFFAGVVGDVTGGLISDKVLKKTG-SLPMARQGVIAFSLLGTLCFLVPVM 182 IK+ A+FA F A +G V GG +S K SL +R+ V+ L+G+L + P Sbjct: 260 IKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSRKLVM---LVGSLSMIGPAC 316 Query: 183 LSH-DVNVVAWC-LSGAFFMLELTIGPIWAVPMDV-APEYTGTASGMLNTGAALATIISP 239 + D VA LS F + G ++++ DV TA+G+ L + Sbjct: 317 VGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTGMSGYLGATLFT 376 Query: 240 VVFGTVIDLTG 250 ++FG ++ G Sbjct: 377 LLFGILVTQIG 387 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.450 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,008,983 Number of extensions: 44188 Number of successful extensions: 234 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 226 Number of HSP's successfully gapped: 6 Length of query: 293 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 209 Effective length of database: 1,222,130 Effective search space: 255425170 Effective search space used: 255425170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)