BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0098.p (210 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309771|ref|YP_003773863.1| thiamine monophosphate synthase... 273 6e-75 >gi|300309771|ref|YP_003773863.1| thiamine monophosphate synthase [Herbaspirillum seropedicae SmR1] Length = 210 Score = 273 bits (699), Expect = 6e-75, Method: Compositional matrix adjust. Identities = 139/202 (68%), Positives = 166/202 (82%) Query: 9 LRGLYIVTPDWDDTDKLVSATEQAIKGGATLVQYRHKTADAAQRKEQAGALLQVCRRHDT 68 LRGLYIVTPDWDDT +L++ATE A++ GA LVQYRHKTADA QR+ QA ALL +CR++ Sbjct: 9 LRGLYIVTPDWDDTAQLLAATELALQQGAALVQYRHKTADAQQRQAQASALLALCRQYQV 68 Query: 69 PFIINDYVDLCIELDAEGIHVGGTDASVAEVRAQVGKDKIVGASCYGSLQLARDAHAAGA 128 P IIND+VDLC+++DA+GIHVGGTDAS+AEVR VG D+IVGASCYG+L+LA A+ GA Sbjct: 69 PLIINDHVDLCLDIDADGIHVGGTDASIAEVRKAVGPDRIVGASCYGTLELAHAAYRDGA 128 Query: 129 TYVAFGGFYPSRVKKYDFKTAPEIVAGSKKEIPLPVVVIGGMTQANCLPLIAQGADMVAA 188 +YVAFGGFYPSRVKKYDF+TAPEI+A SK+EIPLPVVVIGG+T N PL+ QGADMVA Sbjct: 129 SYVAFGGFYPSRVKKYDFRTAPEIIAHSKREIPLPVVVIGGITLENAPPLVEQGADMVAV 188 Query: 189 ISSVYMTDDPEASARAFSQLIR 210 ISSVY+ E R + L R Sbjct: 189 ISSVYLVPVEERKTRELADLYR 210 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 669,188 Number of extensions: 27944 Number of successful extensions: 101 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 101 Number of HSP's successfully gapped: 1 Length of query: 210 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 129 Effective length of database: 1,236,335 Effective search space: 159487215 Effective search space used: 159487215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)