BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0096.p (237 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309770|ref|YP_003773862.1| phosphomethylpyrimidine kinase ... 133 1e-32 >gi|300309770|ref|YP_003773862.1| phosphomethylpyrimidine kinase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 133 bits (335), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 65/76 (85%), Positives = 69/76 (90%) Query: 69 GGAGMQADIPAISALGCHPLSVPTALTVQDNVSVFAVHPIDAELIRHQAQVLIDRFDIRA 128 GGAGMQADIPAI+ALGCHPLSVPTALTVQDNVSVFAVH +D EL+RHQAQVLIDRFDI A Sbjct: 22 GGAGMQADIPAITALGCHPLSVPTALTVQDNVSVFAVHALDPELVRHQAQVLIDRFDIAA 81 Query: 129 VKLGIAGNRRNGEXAA 144 VKLGIAG+R N E A Sbjct: 82 VKLGIAGSRENAEVIA 97 Score = 123 bits (308), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 59/67 (88%), Positives = 63/67 (94%) Query: 1 MQADIPAISALGCHPLSVPTALTVQDNVSVFAVHPIDAELIRHQAQVLIDRFDIRAVKLG 60 MQADIPAI+ALGCHPLSVPTALTVQDNVSVFAVH +D EL+RHQAQVLIDRFDI AVKLG Sbjct: 26 MQADIPAITALGCHPLSVPTALTVQDNVSVFAVHALDPELVRHQAQVLIDRFDIAAVKLG 85 Query: 61 IAGNRRN 67 IAG+R N Sbjct: 86 IAGSREN 92 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 598,372 Number of extensions: 18764 Number of successful extensions: 61 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 61 Number of HSP's successfully gapped: 2 Length of query: 237 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 155 Effective length of database: 1,231,600 Effective search space: 190898000 Effective search space used: 190898000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)