BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0091.p (936 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspi... 1783 0.0 gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herb... 179 7e-46 gi|300309930|ref|YP_003774022.1| leucyl-tRNA synthetase [Herbasp... 76 1e-14 >gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 971 Score = 1783 bits (4617), Expect = 0.0, Method: Compositional matrix adjust. Identities = 845/936 (90%), Positives = 886/936 (94%) Query: 1 MELAKSFEPADIEKFWREEWEKRGYFAATTAPEKPSFSIQLPPPNVTGTLHMGHAFNQTI 60 MELAKSFEPA+IEKFWREEWEKRGYFAATT EKPSFSIQLPPPNVTGTLHMGHAFNQTI Sbjct: 36 MELAKSFEPAEIEKFWREEWEKRGYFAATTDAEKPSFSIQLPPPNVTGTLHMGHAFNQTI 95 Query: 61 MDGLTRYHRMRGFNTAWIPGTDHAGIATQIVVERQLDAQKISRHDLGREKFVEKVWEWKE 120 MDGLTRYHRMRG+NTAWIPGTDHAGIATQIVVERQLDAQK+SRHDLGREKFVEKVWEWKE Sbjct: 96 MDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVWEWKE 155 Query: 121 KSGSTITGQMRRMGASTDWDREYFTMDPKMSNAVTEVFVRLFEQGLIYRGKRLVNWDPVL 180 KSGSTITGQMRRMGASTDW REYFTMDPKMS VTEVFVRL+EQGLIYRGKRLVNWDPVL Sbjct: 156 KSGSTITGQMRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNWDPVL 215 Query: 181 GTAVSDLEVVSEEEDGSMWHIKYPLVDGSGHITVATTRPETMLGDVAVAVDPTDERYAHL 240 GTAVSDLEVVSEEEDGSMWHIKYPL DGSGHI VATTRPETMLGDVAVAVDPTDERYA L Sbjct: 216 GTAVSDLEVVSEEEDGSMWHIKYPLADGSGHIVVATTRPETMLGDVAVAVDPTDERYAAL 275 Query: 241 VGKMLKLPLTDREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLDKISILTLDA 300 VGKM+KLPL REIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNL+ IS+ TL A Sbjct: 276 VGKMIKLPLVGREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKA 335 Query: 301 RINENGPAKYQGLDRFEARKQMVADLDALGLLELVKPHKLMVPRGDRTGVVIEPMLTDQW 360 INEN P KYQGLDRFEARKQ+VADLDALGLLE VKPHKLMVPRGDRTGVVIEPMLTDQW Sbjct: 336 TINENAPEKYQGLDRFEARKQIVADLDALGLLEQVKPHKLMVPRGDRTGVVIEPMLTDQW 395 Query: 361 FVAMSKPAPEGTYFPGKSIAEVALEKVANGEIKLIPENWNNTYNQWLNNIQDWCISRQLW 420 FVAMSKPAPEGT+FPGKSIAE ALEKV++GEIK +PENW NTYNQWLNNIQDWCISRQLW Sbjct: 396 FVAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQLW 455 Query: 421 WGHQIPAWYDEDGKIYVARTEAEARAQAGGKTVTRDNDVLDTWFSSALVPFSTLGWPEET 480 WGHQIPAWYDEDG IYVARTE EARAQAGG+ V RD DVLDTWFSSALVPFSTLGWPEET Sbjct: 456 WGHQIPAWYDEDGGIYVARTEEEARAQAGGRAVKRDEDVLDTWFSSALVPFSTLGWPEET 515 Query: 481 PDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGQVPFNTVYVHGLVRDASGQKMSKSK 540 PDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTG+VPF+TVYVHGLVRDASGQKMSKSK Sbjct: 516 PDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPFDTVYVHGLVRDASGQKMSKSK 575 Query: 541 GNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEFADGIPAFGTDALRFTFA 600 GNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEFADGIPAFGTDALRFTFA Sbjct: 576 GNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEFADGIPAFGTDALRFTFA 635 Query: 601 SLATLGRNINFDLGRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGHVKGECNKEQLQFS 660 SLATLGRNINFDL RCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGH KG C+KE+LQFS Sbjct: 636 SLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGHEKGVCDKEKLQFS 695 Query: 661 QADRWIVSLMQRTEAEVEKGFADYRFDNIASAIYKFVWDEYCDWYLEMAKSQIQNGNEAQ 720 +ADRWIVSL+QRTEAEVEKGFADYRFDNIA+AIYKFVWDEYCDWYLEMAK+QIQ+GNEAQ Sbjct: 696 KADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWDEYCDWYLEMAKAQIQSGNEAQ 755 Query: 721 QRATRRTLLRVLEGVLRLAHPVLPFITEALWQSVAPLTDKKLDPAGDSIMRQPYPQADLE 780 QRATRRTLLRVLE VLRLAHPVLPFITE LWQSVAPLTD+K DPAGDSIMRQ YP+ DLE Sbjct: 756 QRATRRTLLRVLESVLRLAHPVLPFITEQLWQSVAPLTDRKPDPAGDSIMRQAYPRPDLE 815 Query: 781 KIDLDAEAWMTQLKTLTDACRNLRGEMQLSPALRVPLIVEAANAEAVNSFLPYLQSLVKL 840 KID +AEAW+ Q K TDACRNLRGEMQL+P+LRVPLIVEAA+A AV SFLPYLQ LV+L Sbjct: 816 KIDAEAEAWVAQFKAYTDACRNLRGEMQLAPSLRVPLIVEAADASAVESFLPYLQGLVRL 875 Query: 841 SETQVVTQLPESPAPVAIVGDLKLMLKVEIDIAAERERMAKEITRISGEIVKANAKLSNE 900 SET VV QLPESPAPVAIVG++KLMLKVEID+AAERER+ KE+TR++ EI KAN KLSNE Sbjct: 876 SETTVVAQLPESPAPVAIVGEVKLMLKVEIDVAAERERLGKEVTRLTAEIAKANGKLSNE 935 Query: 901 SFVTRAPEAVVAQEKERLANFSTTLAKLEEQLARLK 936 SFV RAPEAVVAQEKER+ANFS TL K++EQLA+LK Sbjct: 936 SFVARAPEAVVAQEKERVANFSATLEKVKEQLAKLK 971 >gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 964 Score = 179 bits (455), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 196/859 (22%), Positives = 341/859 (39%), Gaps = 162/859 (18%) Query: 10 ADIEKFWREEWEKRGYFAAT--TAPEKPSFSIQLPPPNVTGTLHMGHAFNQTIMDGLTRY 67 A E W ++W+++ + + +P F + PP G +H+GHA N+ + D + + Sbjct: 39 AKREPQWVKQWQEKKVYERIRKASAGRPKFILHDGPPYANGEIHLGHAVNKILKDMIVKA 98 Query: 68 HRMRGFNTAWIPGTDHAGIATQIVVERQLDAQKISRHDLGREKFVEKVWEWKEKSGSTIT 127 M GF+ ++PG D G+ +I +E++ +L + K + + + Sbjct: 99 RNMAGFDAQYVPGWDCHGMPIEIQIEKKYGK------NLPVAEVQAKARAYANEQIALQK 152 Query: 128 GQMRRMGASTDWDREYFTMDPKMSNAVTEV--FVRLFEQGLIYRGKRLVNWDPVLGTAVS 185 R+G +WD Y TM+ SN E+ ++ E+G +YRG + VNW G+A++ Sbjct: 153 KDFIRLGVLGEWDNPYKTMN--FSNEADELRALGKILEKGYVYRGLKPVNWCFDCGSALA 210 Query: 186 DLEVVSEEEDGSMWHIKYPLVDG---------------SGHITVATTRPETMLGDVAVAV 230 + EV +++ + +P + G+I + TT P T+ + A+ V Sbjct: 211 EAEVEYQDKRDPAIDVGFPFAEHDKVATAFGLTDLPTRHGYIVIWTTTPWTIPANQALNV 270 Query: 231 DP---------------------------TDERYAHLVGKML------KLPLT------- 250 P ER+ L GK++ KL L Sbjct: 271 HPEFEYALVETSRNGEATLLILAKDLVASCLERFG-LEGKIIATTTGEKLSLIRFHHPLA 329 Query: 251 ------DREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLDKISILTL---DAR 301 DR P+ EYV + GTG V +PA+ D+ H + IL D R Sbjct: 330 KADAGYDRLSPVFLGEYVTADSGTGIVHSSPAYGIEDFVSCKAHGMKDDDILNPVMGDGR 389 Query: 302 INENGPAKYQGLDRFEARKQMVADLDALG-LLELVK-PHKLMVPRGDRTGVVIEPMLTDQ 359 P + GL +EA K + L+ +G L +L+ H M ++ ++ T Q Sbjct: 390 YASWLPL-FAGLTIWEASKPICDKLEEIGSLFKLIMFSHSYMHCWRHKSPIIYRA--TSQ 446 Query: 360 WFVAMSKPAPEGTYFPGKSIAEVALEKVANGEIKLIPENWNNTYNQWLNNIQDWCISRQL 419 WF +M +G G S+ + AL+ + + P + + N DW +SRQ Sbjct: 447 WFASMDNTPKDG----GPSLRQTALQGIED--TAFFPGWGKARLHGMIANRPDWTLSRQR 500 Query: 420 WWGHQIPAW-YDEDGKIYVARTE----AEARAQAGG-----------------KTVTRDN 457 WG + + + E G+++ E R + GG ++ Sbjct: 501 QWGVPMAFFVHKETGELHPRTPELLEQIAQRIEQGGIEAWQALDPKELLGDEADQYLKNK 560 Query: 458 DVLDTWFSSALVPFSTLGWPEETPDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGQV 517 D LD WF S + L + +L P+ + + G D W ++ ++ G+ Sbjct: 561 DTLDVWFDSGSTHQTVL---RGSHRQELQFPADLYLEGSDQHRGWFHSSLLTSSMLNGRA 617 Query: 518 PFNTVYVHGLVRDASGQKMSKSKGNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEK 577 P+ + HG V D G+KMSKS N ++P + D +G + + + V + SI + Sbjct: 618 PYKALLTHGFVVDGEGRKMSKSLKNGVEPQKVADSLGAE--ILRLWVASSDYSGEISISE 675 Query: 578 ATRKEFADGIPAFGTDALRFTFASLATLGRNINFDLGRCEGYRNFCNKLWNATRFVLMNT 637 K R T E YR N L RF+L NT Sbjct: 676 EILK--------------RVT------------------EAYRRIRNTL----RFLLANT 699 Query: 638 EGQDCGFDGHVKGECNKEQLQFSQADRWIVSLMQRTEAEVEKGFADYRFDNIASAIYKFV 697 FD E L+ DR+ ++ M R + EV F Y F + + + + Sbjct: 700 S----DFDPAQHAVPMAELLEI---DRYAIANMARLQGEVLAHFETYEFHPVVAKLQMYC 752 Query: 698 WDEYCDWYLEMAKSQIQNG--NEAQQRATRRTLLRVLEGVLRLAHPVLPFITEALWQSVA 755 ++ +YL++ K ++ + A +R+ + + + + +LRL P+L F E W A Sbjct: 753 SEDLGGFYLDILKDRLYTSGVDSAARRSAQTAVWHITQSLLRLMAPILSFTAEEAWSFFA 812 Query: 756 PLTDKKLDPAGDSIMRQPY 774 + + +G++I Q Y Sbjct: 813 --GKEAFEASGETIFSQVY 829 >gi|300309930|ref|YP_003774022.1| leucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 882 Score = 76.3 bits (186), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 53/203 (26%), Positives = 86/203 (42%), Gaps = 26/203 (12%) Query: 3 LAKSFEPADIEKFWREEWEKRGYFAAT------TAPEKPSFSIQLPPPNVTGTLHMGHAF 56 + + PA++E+ ++ W + +K F P +G LHMGH Sbjct: 1 MQDKYSPAEVEQSAQQHWNAIDAYKTVENARDKQGRDKKKFYACSMLPYPSGKLHMGHVR 60 Query: 57 NQTIMDGLTRYHRMRGFNTAWIPGTDHAGIATQIVVERQLDAQKISRHDLGREKFVEKVW 116 N TI D + RY RM G+N G D G+ E A + Sbjct: 61 NYTINDVMYRYLRMNGYNVLMPMGWDAFGMPA----ENAAMANGVPPA------------ 104 Query: 117 EWKEKSGSTITGQMRRMGASTDWDREYFTMDPKMSNAVTEVFVRLFEQGLIYRGKRLVNW 176 +W + + QM+ MG + DW RE P+ +F+++ E+G+IY+ VNW Sbjct: 105 QWTYSNIDYMKSQMQSMGLAIDWSREMTACRPEYYKWNQWMFLKMLEKGIIYKKTGTVNW 164 Query: 177 DPVLGTAVSDLEVVSEEEDGSMW 199 DP+ T +++ +V+ DG W Sbjct: 165 DPIDQTVLANEQVI----DGRGW 183 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,823,725 Number of extensions: 115867 Number of successful extensions: 301 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 295 Number of HSP's successfully gapped: 4 Length of query: 936 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 842 Effective length of database: 1,174,780 Effective search space: 989164760 Effective search space used: 989164760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)