BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0090.p (227 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309766|ref|YP_003773858.1| signal peptide protein [Herbasp... 199 2e-52 >gi|300309766|ref|YP_003773858.1| signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 154 Score = 199 bits (506), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 94/114 (82%), Positives = 107/114 (93%) Query: 23 WADGSPVGLWKNIDDATGKPKALIRITESDGELKGKIEKLFRPAEEEQNPKCEKCEGALK 82 WADGSPVGLWK+IDDATGKPKALIRI+E++GEL GKIEKLFR EE+QNP C+KCEG LK Sbjct: 27 WADGSPVGLWKSIDDATGKPKALIRISENNGELSGKIEKLFRAPEEDQNPVCQKCEGELK 86 Query: 83 DQPIIGMTILSGLKKDGDEYNGGQILDPANGKLYKSKLTVVEDGKKLNVRGXGG 136 D+PIIGMTILSGLKKDGDEYNGGQI+DPANGK+YKSKL+V++DG+KLNVRG G Sbjct: 87 DKPIIGMTILSGLKKDGDEYNGGQIIDPANGKVYKSKLSVIDDGQKLNVRGYVG 140 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 511,589 Number of extensions: 17893 Number of successful extensions: 43 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 43 Number of HSP's successfully gapped: 1 Length of query: 227 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 145 Effective length of database: 1,231,600 Effective search space: 178582000 Effective search space used: 178582000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)