BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0078.p (117 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309754|ref|YP_003773846.1| tRNA-dihydrouridine synthase or... 198 9e-53 >gi|300309754|ref|YP_003773846.1| tRNA-dihydrouridine synthase or NifR3-like protein [Herbaspirillum seropedicae SmR1] Length = 340 Score = 198 bits (504), Expect = 9e-53, Method: Compositional matrix adjust. Identities = 91/116 (78%), Positives = 103/116 (88%) Query: 1 MIGRAAQGRPWIFREIDHFLRTGTHLPAPMVAEVQELMLEHLQAHYAFYGEYLGVRTARK 60 M+GRAAQGRPWIFREIDHFLRTG HLPAP+V+EV++LM EHLQAHYAFYG+YLGVRTARK Sbjct: 222 MVGRAAQGRPWIFREIDHFLRTGEHLPAPLVSEVEQLMDEHLQAHYAFYGDYLGVRTARK 281 Query: 61 HIGWYVRDLPEGEQFRQRMNRLEDTQLQWNAVRDFFESQAAYGERLQYGIAGEALA 116 HIGWYVRDLP GEQFR+ MNR+ED Q AVR FF SQAA+GERLQYG+A + +A Sbjct: 282 HIGWYVRDLPGGEQFRRSMNRIEDCAQQLQAVRQFFASQAAFGERLQYGLAVDEVA 337 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 393,323 Number of extensions: 15406 Number of successful extensions: 52 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 52 Number of HSP's successfully gapped: 1 Length of query: 117 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 43 Effective length of database: 1,269,480 Effective search space: 54587640 Effective search space used: 54587640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 124 (52.4 bits)