BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0049.p (180 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309724|ref|YP_003773816.1| transmembrane protein [Herbaspi... 258 3e-70 >gi|300309724|ref|YP_003773816.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 179 Score = 258 bits (658), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 136/179 (75%), Positives = 161/179 (89%), Gaps = 1/179 (0%) Query: 1 VLHSIFMLIVDAIASVLAGVLLLRFWMQVVQVRPPASVGQFVFQLSDWLVKPLRRIFPGV 60 +LHSIFMLIV+A+AS+LAGV+LLRFWMQVV+VRPP SVGQFV+QLSDWLVKPLRR+ PG Sbjct: 1 MLHSIFMLIVNAVASILAGVMLLRFWMQVVRVRPPQSVGQFVYQLSDWLVKPLRRVLPG- 59 Query: 61 GGYDWASLVGAFLVILLSIIAALGFASGFDFQPIILFSLVRFFQWIFYGFMATLIIEAIF 120 G YDWASL+GAFL+++L+ +AA+G SGFDF+ I L SL RF +WI YGFMATLIIEAIF Sbjct: 60 GAYDWASLLGAFLIVVLATVAAVGLQSGFDFKIIFLLSLERFVEWICYGFMATLIIEAIF 119 Query: 121 SWVNPYAPLAPFVRALNEPLLRPLRKVLPLIGNVDLSPLAALILLQIAVRLVSTLILSI 179 SWVNPYAPLAPFVRALNEPLLRPLR+V+P +G VDLSPL LILLQIAVR++STL+ ++ Sbjct: 120 SWVNPYAPLAPFVRALNEPLLRPLRRVIPPLGGVDLSPLVLLILLQIAVRVISTLLFAL 178 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.334 0.149 0.463 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 533,072 Number of extensions: 20713 Number of successful extensions: 123 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 121 Number of HSP's successfully gapped: 1 Length of query: 180 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 101 Effective length of database: 1,245,805 Effective search space: 125826305 Effective search space used: 125826305 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 127 (53.5 bits)