BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0033.p (141 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309712|ref|YP_003773804.1| hypothetical protein Hsero_0371... 117 4e-28 >gi|300309712|ref|YP_003773804.1| hypothetical protein Hsero_0371 [Herbaspirillum seropedicae SmR1] Length = 137 Score = 117 bits (293), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 63/132 (47%), Positives = 86/132 (65%), Gaps = 3/132 (2%) Query: 10 RTGLRNTAAALSIAAAGLLATTCAFAAGKSNAELDAQYQREKAVCTSGQSNQDKATCLRE 69 R L +A+AL I A L + AAGK++ ++ YQ++ A C +G S++D+ATCLRE Sbjct: 9 RHQLLRSASALVIGLAALGGASHVLAAGKTD---NSDYQQQVAACKNGSSSEDRATCLRE 65 Query: 70 AGAAYQQARQGRLNDGQQAQYSSNALERCKALPAGDQADCQRRIENPSDVEGSVPSGGIL 129 AGAA Q A +G L D AQ NA++RC+ LP D+ DC++R+ VEGSV GGIL Sbjct: 66 AGAARQAASRGNLTDPGAAQLKENAMKRCEGLPQSDRIDCEKRVNGEGRVEGSVSEGGIL 125 Query: 130 RKDVTIVPATTQ 141 R+ VTIVPAT + Sbjct: 126 RETVTIVPATAK 137 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.124 0.349 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 340,697 Number of extensions: 9877 Number of successful extensions: 21 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 21 Number of HSP's successfully gapped: 1 Length of query: 141 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 65 Effective length of database: 1,260,010 Effective search space: 81900650 Effective search space used: 81900650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 126 (53.1 bits)