BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0004.p (189 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 155 1e-39 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 154 4e-39 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 141 3e-35 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 133 8e-33 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 114 7e-27 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 155 bits (393), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 77/182 (42%), Positives = 107/182 (58%), Gaps = 4/182 (2%) Query: 4 ASKAKLGDPTLPDVQVGPVATRPQFEKVMEYIGIAKNEGARCVFGGNARTGEGYGAGQFV 63 A K +LG P +VGP+ R Q+E + + GA + G+ R GE G FV Sbjct: 323 AEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGA-TLAAGHTRYGE---EGYFV 378 Query: 64 EPTIFVDVSNDMRIAQEEVFGPVLSVIKFKDEEDAIRIANDIRFGLASGVWTTSLHRAML 123 PT+ SN M +A+ E+FGPV I F+DEE+AI IAND FGLA VWT + RA Sbjct: 379 RPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTRDVARAHR 438 Query: 124 MSSRIKAGTVWINNYRSTSFTTPFGGYKDSGTGREGGIEAVKEYLDTKSVWISTDLDMPD 183 +++ + AGT W+N+Y++ + +PFGG+ SG GR G+EA+ +Y TKSVW+ T Sbjct: 439 VAASVNAGTFWVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQTKSVWVETSASPAT 498 Query: 184 PF 185 PF Sbjct: 499 PF 500 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 154 bits (390), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 77/165 (46%), Positives = 110/165 (66%), Gaps = 4/165 (2%) Query: 9 LGDPTLPDVQVGPVATRPQFEKVMEYIGIAKNEGARCVFGGNARTGEGYGAGQFVEPTIF 68 +GDP+ P V++GPVA + QFE+V YI +EGA + GG +R +G+ FV+PTIF Sbjct: 318 VGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPDKGW----FVQPTIF 373 Query: 69 VDVSNDMRIAQEEVFGPVLSVIKFKDEEDAIRIANDIRFGLASGVWTTSLHRAMLMSSRI 128 D +N M IA+EE+FGPV ++I F EE+AI +AND ++GLA+ VWTT+L RA +++ + Sbjct: 374 ADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGV 433 Query: 129 KAGTVWINNYRSTSFTTPFGGYKDSGTGREGGIEAVKEYLDTKSV 173 K G V +N + P+GG KDSG GREGG+ Y + K+V Sbjct: 434 KVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTV 478 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 141 bits (356), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 75/170 (44%), Positives = 100/170 (58%), Gaps = 2/170 (1%) Query: 8 KLGDPTLPDVQVGPVATRPQFEKVMEYIGIAKNEGARCVFGG-NARTGEGYGAGQFVEPT 66 K G+P +G A++ Q EK++ YI I K EGA+ + GG G +G +V+PT Sbjct: 329 KQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREELGGDLASGYYVKPT 388 Query: 67 IFVDVSNDMRIAQEEVFGPVLSVIKFKDEEDAIRIANDIRFGLASGVWTTSLHRAMLMSS 126 +F +N MRI QEE+FGPV+SV FKDEE+A+ IAND +GL +G+WT RA M Sbjct: 389 VF-QGNNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAFRMGR 447 Query: 127 RIKAGTVWINNYRSTSFTTPFGGYKDSGTGREGGIEAVKEYLDTKSVWIS 176 I+AG VW N Y FGGYK SG GRE + Y TK++ +S Sbjct: 448 EIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNLLVS 497 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 133 bits (335), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 70/173 (40%), Positives = 105/173 (60%), Gaps = 2/173 (1%) Query: 1 VAIASKAKLGDPTLPDVQVGPVATRPQFEKVMEYIGIAKNEGARCVFGGNARTGEGYGAG 60 +A ++ K+GDP GP+A R QF +V + I +GAR + GG R +G G Sbjct: 311 LATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRP-DGLAQG 369 Query: 61 QFVEPTIFVDVSNDMRIAQEEVFGPVLSVIKFKDEEDAIRIANDIRFGLASGVWTTSLHR 120 + PTIF +V DM IAQEE+FGPVL+++ + E+AI IAND +GL + V + Sbjct: 370 YYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQ 429 Query: 121 AMLMSSRIKAGTVWINNYRSTSFTTPFGGYKDSGTGREGGIEAVKEYLDTKSV 173 +++RI++G V + NY + PFGGYK SG GRE G+E ++EY++ K++ Sbjct: 430 VRAVAARIQSGQVHL-NYPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKAI 481 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 114 bits (284), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 58/166 (34%), Positives = 92/166 (55%) Query: 8 KLGDPTLPDVQVGPVATRPQFEKVMEYIGIAKNEGARCVFGGNARTGEGYGAGQFVEPTI 67 K+G T P Q+GP+ K+ +++ A GAR + GG G G+G+ + PT+ Sbjct: 323 KVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQGPGFGSDNYYAPTV 382 Query: 68 FVDVSNDMRIAQEEVFGPVLSVIKFKDEEDAIRIANDIRFGLASGVWTTSLHRAMLMSSR 127 DV+ M+ + EE FGPV + +F E++ I AN FGLA+ ++T + R ++ Sbjct: 383 LADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYFYSTDVRRIHRVTDA 442 Query: 128 IKAGTVWINNYRSTSFTTPFGGYKDSGTGREGGIEAVKEYLDTKSV 173 +++G V +N + PFGG K+SG GREG + + +YL TK V Sbjct: 443 LESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDYLHTKYV 488 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 637,080 Number of extensions: 26665 Number of successful extensions: 95 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 76 Number of HSP's successfully gapped: 19 Length of query: 189 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 109 Effective length of database: 1,241,070 Effective search space: 135276630 Effective search space used: 135276630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)