BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0002.p (307 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 234 5e-63 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 221 7e-59 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 213 2e-56 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 205 4e-54 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 204 7e-54 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 234 bits (598), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 123/298 (41%), Positives = 165/298 (55%), Gaps = 1/298 (0%) Query: 6 QLFINNEWVDPLSGQWFDSLDPFSGEAWAQVPRANKDDVNRAVEAASAAMEGPWGTMSAT 65 Q FI N WV P SG+ LDP G+ + + R N D+++AV AA A EG WG +SA Sbjct: 3 QHFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLSAA 62 Query: 66 DRGLLLHKLGTLIEKHADTLTLAESRDNGKLKSEVGGQVRYMAKYFYYYGGLADKIQGAV 125 +R LL KL ++ H + L E RD GK + + +YF +Y G ADK+ G Sbjct: 63 ERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHGDT 122 Query: 126 IPMDKPKVFNYTRYEPIGVVATITPWNSSLLLTAWKIAPALCAGNTIIAKPSEHTSVSMF 185 IP T EP GV I PWN + + + AL AGNT + KP+E +S+ Sbjct: 123 IPYQAGYTV-LTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSIL 181 Query: 186 ELARLFIEAGFPPGVFNVITGFGAEVGEPLVTHPKVPRIAFTGGDEGGRKVNVLGAQHFK 245 +A L E GFP G N++TG+G E G+ LV HP V I+FTG G+ V A+H Sbjct: 182 RVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHT 241 Query: 246 RVSLELGGKSPHIIFDDADLTRAANGVITGIFSAGGQTCMAGSRLLLQEGIHDEFVLR 303 V+LELGGKSP I+F+DAD+ V+ I GQTC AGSRLL+Q G +++ + R Sbjct: 242 PVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLER 299 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 221 bits (562), Expect = 7e-59, Method: Compositional matrix adjust. Identities = 131/306 (42%), Positives = 173/306 (56%), Gaps = 7/306 (2%) Query: 1 MTQRYQLFINNEWVDPLSGQWFDSLDPFSGEAWAQVPRANKDDVNRAVEAASAAMEG-PW 59 M+ Q FI E + GQ + ++P + E +A + DV RAV AA A +EG W Sbjct: 1 MSLHLQHFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAW 60 Query: 60 GTMSATDRGLLLHKLGTLIEKHADTLTLAESRDNGKLKSEVGG-QVRYMAKYFYYYGGLA 118 +S RG LLHKL L+E+ D L ++R G+ E V + G A Sbjct: 61 SKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWA 120 Query: 119 DKIQGAVIP----MDKPKVFNYTRYEPIGVVATITPWNSSLLLTAWKIAPALCAGNTIIA 174 ++++G IP M KP +YT EPIGVV I PWN+ L++T+WK+A L AG T++ Sbjct: 121 NQMEGRTIPTAGYMGKP-TLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVI 179 Query: 175 KPSEHTSVSMFELARLFIEAGFPPGVFNVITGFGAEVGEPLVTHPKVPRIAFTGGDEGGR 234 KP+E T S LARL EAGFP GV NV+TG+G VG L HP V +I+FTG E GR Sbjct: 180 KPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGR 239 Query: 235 KVNVLGAQHFKRVSLELGGKSPHIIFDDADLTRAANGVITGIFSAGGQTCMAGSRLLLQE 294 + + + FKRV+LELGGKSP I+FDDA A G G+F GQ C AGSR+L+Q Sbjct: 240 AIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQR 299 Query: 295 GIHDEF 300 + F Sbjct: 300 SLAQRF 305 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 213 bits (541), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 112/309 (36%), Positives = 177/309 (57%), Gaps = 9/309 (2%) Query: 3 QRYQLFINNEWVDPLSGQWFDSLDPFSGEAWAQVPRANKDDVNRAVEAASAAMEGPWGTM 62 QRY FI ++V P+ G++F+++ P G A+ +V R++ +DV A++AA AA + WG Sbjct: 17 QRYDNFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKS-WGKT 75 Query: 63 SATDRGLLLHKLGTLIEKHADTLTLAESRDNGK-LKSEVGGQVRYMAKYFYYYGGLADKI 121 S T+R +L K+ +E + + L AE+ DNGK ++ + + +F Y+ Sbjct: 76 SPTERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQ 135 Query: 122 QGAVIPMDKPKVFNYTRYEPIGVVATITPWNSSLLLTAWKIAPALCAGNTIIAKPSEHTS 181 +G++ P+D + Y +EP+GVV I PWN +L+ WK+APAL AGN ++ KP+E T Sbjct: 136 EGSICPIDN-DTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTP 194 Query: 182 VSMFELARLFIEAGFPPGVFNVITGFGAEVGEPLVTHPKVPRIAFTGGDEGGRKVNVLGA 241 S+ L L + PPGV N++ GFG E G+PL ++ ++ +IAFTG GR + + Sbjct: 195 ASIMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYAS 253 Query: 242 QHFKRVSLELGGKSPHIIFDDADLTRAANGVIT----GIFSAG-GQTCMAGSRLLLQEGI 296 Q+ V+LELGGKSP+I F D +F+ G+ C SR+L+QE I Sbjct: 254 QNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEVCTCPSRVLVQESI 313 Query: 297 HDEFVLRTL 305 ++ F+ R L Sbjct: 314 YERFIERAL 322 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 205 bits (521), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 108/306 (35%), Positives = 170/306 (55%), Gaps = 1/306 (0%) Query: 1 MTQRYQLFINNEWVDPLSGQWFDSLDPFSGEAWAQVPRANKDDVNRAVEAASAAMEGPWG 60 M + QLFIN EW SG+ D ++P + E ++ A++ D++RA+EAAS E W Sbjct: 1 MYKDVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFET-WR 59 Query: 61 TMSATDRGLLLHKLGTLIEKHADTLTLAESRDNGKLKSEVGGQVRYMAKYFYYYGGLADK 120 MSA +R ++ + L+ + A+ + + + GK +E + A ++ A + Sbjct: 60 NMSAFERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARR 119 Query: 121 IQGAVIPMDKPKVFNYTRYEPIGVVATITPWNSSLLLTAWKIAPALCAGNTIIAKPSEHT 180 G ++P P + EP+G VA TPWN L K++ AL AG +II K E T Sbjct: 120 TYGRLVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEET 179 Query: 181 SVSMFELARLFIEAGFPPGVFNVITGFGAEVGEPLVTHPKVPRIAFTGGDEGGRKVNVLG 240 S EL R + +AG P GV N++ G AE+ E L+ HP + +I+FTG G+++ L Sbjct: 180 PASPAELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALA 239 Query: 241 AQHFKRVSLELGGKSPHIIFDDADLTRAANGVITGIFSAGGQTCMAGSRLLLQEGIHDEF 300 QH KR ++ELGG +P ++FDDAD+ AA + T F GQ C++ +R L+Q+G+ + F Sbjct: 240 GQHMKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAF 299 Query: 301 VLRTLT 306 V + +T Sbjct: 300 VDKFVT 305 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 204 bits (519), Expect = 7e-54, Method: Compositional matrix adjust. Identities = 127/300 (42%), Positives = 177/300 (59%), Gaps = 16/300 (5%) Query: 8 FINNEWVDPLSGQWFDSLDPFSGEAWAQVPRANKDDVNRAVEAASAAMEGPWGTMSATDR 67 FI + P + D +DP SG+ A + + + DV+ AV+AA AA+ G W + R Sbjct: 36 FIAGRFTQPKAD--LDDIDPASGKLLAHLSQGSAADVDAAVQAAQAALPG-WQALGGHGR 92 Query: 68 GLLLHKLGTLIEKHADTLTLAESRDNGK-LKSEVGGQVRYMAKYFYYYGGLADKIQGAVI 126 L+ L +++HA L + E+ DNGK L+ V +A++FY+Y G A ++Q + Sbjct: 93 ARHLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAGWA-QLQESEF 151 Query: 127 PMDKPKVFNYTRYEPIGVVATITPWNSSLLLTAWKIAPALCAGNTIIAKPSEHTSVSMFE 186 P + P+GVV I PWN LL+ AWKIAPAL GNT++ KP+E+TS++ Sbjct: 152 P----------DHVPVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAENTSLTALL 201 Query: 187 LARLFIEAGFPPGVFNVITGFGAEVGEPLVTHPKVPRIAFTGGDEGGRKVNVLGAQHFKR 246 A L +AG P GV N++TG GA G +V HP + +IAFTG E GR + A K Sbjct: 202 FAELAQQAGLPAGVLNIVTGDGA-TGAAVVAHPGIQKIAFTGSTEVGRLIREQTAGSGKS 260 Query: 247 VSLELGGKSPHIIFDDADLTRAANGVITGIFSAGGQTCMAGSRLLLQEGIHDEFVLRTLT 306 ++LELGGKSP I+F+DAD+ A GV+ I+ GQ C AGSRLL+QEGIHD F+ R T Sbjct: 261 LTLELGGKSPFIVFEDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARLKT 320 Score = 68.9 bits (167), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 66/249 (26%), Positives = 101/249 (40%), Gaps = 8/249 (3%) Query: 6 QLFINNEWVDPLSGQWFDSLDPFSGEAWAQVPRANKDDVNRAVEAASAAMEGPWGTMSAT 65 +L+I + V P G+ + GE V N+ D+ AV AA A W + S Sbjct: 519 KLYIGGKQVRP-DGEVSMACHSTQGERLEDVGLGNRKDIRNAVAAAVKAGG--WTSASPH 575 Query: 66 DRGLLLHKLGTLIEKHADTLTLAESRDNGKLKSEVGGQVRYMAKYFYYYGGLADKIQGAV 125 R L+ + + + + G E +V K + YG ADK +GAV Sbjct: 576 RRAQGLYYIAENLSARTEEFARRIASTTGVTADEARAEVDASIKRLFTYGAWADKFEGAV 635 Query: 126 --IPMDKPKVFNYTRYEPIGVVATITPWNSSLLLTAWKIAPALCAGNTIIAKPSEHTSVS 183 PM + E IGVV + P LL +AP + GN ++ PSE + Sbjct: 636 HQPPM---RGVALAMPEAIGVVGVVCPEEKPLLALISLVAPLIAMGNRVVVVPSEQAPLV 692 Query: 184 MFELARLFIEAGFPPGVFNVITGFGAEVGEPLVTHPKVPRIAFTGGDEGGRKVNVLGAQH 243 +L ++ + P GV N++TG AE+ L H +V + G E L + Sbjct: 693 ATDLYQVLDTSDLPAGVINIVTGKSAELLPVLAEHDEVEALWVRGPAEFSATAERLSTGN 752 Query: 244 FKRVSLELG 252 KR ++ G Sbjct: 753 LKRCFVDHG 761 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,047,650 Number of extensions: 46474 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 19 Length of query: 307 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 222 Effective length of database: 1,217,395 Effective search space: 270261690 Effective search space used: 270261690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)