BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0095.p (553 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 962 0.0 gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of... 189 6e-49 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 185 1e-47 gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 184 2e-47 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 180 2e-46 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 962 bits (2488), Expect = 0.0, Method: Compositional matrix adjust. Identities = 498/552 (90%), Positives = 531/552 (96%), Gaps = 3/552 (0%) Query: 1 MATAS---TAHTITPEERKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI 57 M TAS TA ITPEE+KVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI Sbjct: 1 MTTASVNTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI 60 Query: 58 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGILPNYNSIGIAA 117 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVG+LPNYNSIGI A Sbjct: 61 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA 120 Query: 118 PIILIILRILQGLALGGEYGGAATYVAEHAPEGKRGSFTAWIQTTATLGLFLSLLVILGV 177 PIILIILRILQGLALGGEYGGAATYVAEHAPEG+RGSFTAWIQTTAT+GLFLSLLVILGV Sbjct: 121 PIILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGV 180 Query: 178 RTAVGEAAFADWGWRIPFLVSIILLGISVWIRLSMNESPAFLKMKSEGKLSKAPLTEAFG 237 RTAVGEAAFADWGWRIPFLVSI+LL ISVWIR+SMNESPAFLKMK+EGK SKAPLTEAFG Sbjct: 181 RTAVGEAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFG 240 Query: 238 KWKNLKLVILALIGMTAGQAVVWYAGQFYSLFFLTQTLKVDGPLANILIALALLLATPFF 297 +WKNLKLVILAL+G+TAGQAVVWY GQFY+LFFLTQTLKVDGP ANILIA ALLLATPFF Sbjct: 241 QWKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANILIAAALLLATPFF 300 Query: 298 LVFGSLSDKIGRKPIILGGCLIAAVTYFPIFQGLTHYANPALEAALKKAPVIVTADPATC 357 L FGSLSDKIGRKPIILGGCLIAAVTYFPIF GLTH+ANPALEAAL+K+PVIVTADPA+C Sbjct: 301 LFFGSLSDKIGRKPIILGGCLIAAVTYFPIFSGLTHFANPALEAALQKSPVIVTADPASC 360 Query: 358 QFQFNPTGTKKFTSSCDIAKAKLSAASVNYENVAGAPGSVATIKIGEKILTSYDAAGLSK 417 QFQFNPTGTKKFTSSCDI KAKLSAASVNY+NVAGAPGSVAT+KIG++I+TSYDAAGLSK Sbjct: 361 QFQFNPTGTKKFTSSCDIVKAKLSAASVNYQNVAGAPGSVATVKIGDRIITSYDAAGLSK 420 Query: 418 EDASAKDKAFSKELADDIKAAGYPTKADPDQMNKPMVLLLLFILVLYVTMVYGPVAAALV 477 EDA+AKDK SK+LADDIKAAGYP+KADP+QMNKPMVLLLLFILV+YVTMVYGP+AA LV Sbjct: 421 EDAAAKDKQLSKDLADDIKAAGYPSKADPEQMNKPMVLLLLFILVIYVTMVYGPIAACLV 480 Query: 478 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTTAFALVAYKGDIYYGLWYPIVIALATFIIGL 537 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTT+FALVA+KGDIYYGLWYPIVIA+ TF+IG+ Sbjct: 481 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTTSFALVAFKGDIYYGLWYPIVIAMITFVIGM 540 Query: 538 LFVKESKDNNIN 549 LFVKE+ NNI+ Sbjct: 541 LFVKETVRNNIH 552 >gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 189 bits (479), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 121/348 (34%), Positives = 192/348 (55%), Gaps = 16/348 (4%) Query: 4 ASTAHTITP----EERKVIFA---SSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAF 56 + T TP E RK IFA +S G + EW+DFY+Y A A FF DP + Sbjct: 2 SDTTQASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQL 61 Query: 57 IFALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGILPNYNSIGIA 116 + FAAGF++RP G +FGR+ D GRK + L+++L+M + V ++P Y +IG Sbjct: 62 LNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGAW 121 Query: 117 APIILIILRILQGLALGGEYGGAATYVAEHAPEGKRGSFTAWIQTTATLGLFLSLLVILG 176 AP +L++ R+ QGL++GGEYG +ATY++E AP G+RG F ++ T G L++LV+ G Sbjct: 122 APALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFG 181 Query: 177 VRTAVGEAAFADWGWRIPFLVSIILLGISVWIRLSMNE-SPAFLKMKSEGKLSKAPLTEA 235 ++ + +A WGWR+PF++ + +++++R S+ E S A + K + K L Sbjct: 182 MQQWLTKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQH- 240 Query: 236 FGKWKNLKLVILALIGMTAGQAVVWYAGQFYSLFFLTQTLKVDGPLANILIALALLLATP 295 K L ++G TAG ++++Y Y +L T +D +AN ++ AL + Sbjct: 241 -------KRAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMI 293 Query: 296 FFLVFGSLSDKIGRKPIILGGCLIAAVTYFPIFQGLTHYANPALEAAL 343 +FG++SDKIGR+ +L V FPI L ++P + AL Sbjct: 294 LQPIFGAISDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMAL 341 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 185 bits (469), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 110/319 (34%), Positives = 177/319 (55%), Gaps = 12/319 (3%) Query: 13 EERKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIFALLAFAAGFIVRPF 72 + R+ I A+ +G EW+DF +Y A IIAK FF + T+ + A+ F GF +RP Sbjct: 18 QRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFMRPV 77 Query: 73 GALVFGRLGDLVGRKYTFLVTILIMGASTFIVGILPNYNSIGIAAPIILIILRILQGLAL 132 G ++ G D VGRK VTIL+M T ++G+ P Y SIG+ AP+I+++ R+LQG + Sbjct: 78 GGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSA 137 Query: 133 GGEYGGAATYVAEHAPEGKRGSFTAWIQTTATLGLFLSLLVILGVRTAVGEAAFADWGWR 192 GGE G A ++ E+APE +R +++WIQ + + + L V V ++ AA A WGWR Sbjct: 138 GGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGWR 197 Query: 193 IPFLVSIILLGISVWIRLSMNESPAFLKMKSEGKLSKAPLTEAFGKWKNLKLVILALIGM 252 +PFL+ II+ + +IR + E+P FL+ K + + +PL E +N A M Sbjct: 198 LPFLLGIIIGPVGYYIRHHLEETPTFLEEKDK---TDSPLKEVI---RNFPRETSASFSM 251 Query: 253 TAGQAVVWYAGQFYSLFFLTQTLKV---DGPLANILIALALLLATPFFLVFGSLSDKIGR 309 V Y FY + + LK+ G +A ++ L +++ P + G L+D+IGR Sbjct: 252 VILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAP---IVGRLADRIGR 308 Query: 310 KPIILGGCLIAAVTYFPIF 328 +P++ G + + +P+F Sbjct: 309 RPLLSGSAALILMLAYPMF 327 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 184 bits (466), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 117/332 (35%), Positives = 185/332 (55%), Gaps = 18/332 (5%) Query: 2 ATASTAHTITPEERKVIFASSLGTVFEWYDFYLYGSLAAIIAKQ-FFAGTDPNTAFIFAL 60 +T S AH + +K + +G+ E+YDF++Y + A++I Q FF DP TA I +L Sbjct: 3 STQSNAHH--QQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASL 60 Query: 61 LAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGILPNYNSIGIAAPII 120 + G++ RP GA V G LGD GRK L + +MG ST VG+LP Y+ +G+ AP + Sbjct: 61 ATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPAL 120 Query: 121 LIILRILQGLALGGEYGGAATYVAEHAPEGKRGSFTAWIQTTATLGLFLSLLVILGVRTA 180 L+I+R+ QG A+ GE GA++ + EH+P G+RG F+++ G L+ V L + Sbjct: 121 LVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHY 180 Query: 181 VGEAAFADWGWRIPFLVSIILLGISVW-IRLSMNESPAFLKMKSEGKLSKAPLTEAFGKW 239 + F WGWRIPFL+S +++ I+ W IR +++E+P F E ++P+ +AF Sbjct: 181 MPAEQFNVWGWRIPFLLSFLVI-IAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDC 239 Query: 240 --KNLKLVILALIGMTAGQAVVW---YAGQ-FYSLFFLTQTLKVDGPLANILIALALLLA 293 L++V +AL+ + A ++ YA Q Y + F + NI+ L + Sbjct: 240 SPNMLRVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVI--- 296 Query: 294 TPFFLVFGSLSDKIGRKPIILGGCLIAAVTYF 325 P+ G LSD+IGR+P I+ G L+A + F Sbjct: 297 -PY---VGKLSDRIGRRPPIIVGSLLAGLFAF 324 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 180 bits (457), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 107/338 (31%), Positives = 183/338 (54%), Gaps = 21/338 (6%) Query: 3 TASTAHTIT-PEERKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIFALL 61 TA+ IT P+ R+ I +SS+G EW+D +YG+ A +IAKQFF D + + + Sbjct: 5 TAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFA 64 Query: 62 AFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGILPNYNSIGIAAPIIL 121 F F +RP GA+V G D GRK +++I++M T ++ +P+Y SIG+ AP + Sbjct: 65 TFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGI 124 Query: 122 IILRILQGLALGGEYGGAATYVAEHAPEGKRGSFTAWIQTTATLGLFLSLLVILGVRTAV 181 + +++QG + GGE+G + ++ EHAP +RG F++W + + L L+ L + + Sbjct: 125 ALGKMIQGFSAGGEFGSSTAFLVEHAPH-RRGFFSSWQVASQGISLLLAALFGAVLNNML 183 Query: 182 GEAAFADWGWRIPFLVSIILLGISVWIRLSMNESPAFLKMKSEGKLSKAPLTEAFGKWKN 241 A WGWR+PF+ +++ ++IR ++E+P F K + + APL + F K Sbjct: 184 TPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEF---KESSEKTNAPLRDTFAHQK- 239 Query: 242 LKLVILALIGMTAGQAVVWYAGQFYSLFFLTQTLKVDG-----PLANILIA-LALLLATP 295 ++L+I AG ++ + SL+ T +K G A + +A L + + +P Sbjct: 240 MRLLI------GAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSP 293 Query: 296 FFLVFGSLSDKIGRKPIILGGCLIAAVTYFPIFQGLTH 333 + G+LSDKIGR P ++ + V +P+F LT+ Sbjct: 294 ---LVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTN 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.140 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,469,751 Number of extensions: 52918 Number of successful extensions: 331 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 305 Number of HSP's successfully gapped: 17 Length of query: 553 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 463 Effective length of database: 1,193,720 Effective search space: 552692360 Effective search space used: 552692360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)