BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0080.p (397 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311530|ref|YP_003775622.1| ABC transporter ATPase [Herbasp... 707 0.0 gi|300311473|ref|YP_003775565.1| ABC transporter ATPase [Herbasp... 211 6e-56 gi|300310722|ref|YP_003774814.1| ABC transporter ATPase [Herbasp... 167 1e-42 gi|300313989|ref|YP_003778081.1| ABC transporter ATPase [Herbasp... 148 6e-37 gi|300311361|ref|YP_003775453.1| antimicrobial peptide ABC trans... 77 3e-15 >gi|300311530|ref|YP_003775622.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 707 bits (1824), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/357 (94%), Positives = 346/357 (96%) Query: 1 MQFGPKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDTNERLGK 60 MQFG KPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLD NERLGK Sbjct: 9 MQFGAKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDVNERLGK 68 Query: 61 LRQDQFAYEEMRVLDVVMMGHTEMWAAMAERDAIYANPEATDDDYMKAADLEAKFAEYDG 120 LRQDQFAYEEMRVLDVVMMGH EMWAAM+ERDAIYANP+ATDDDYMKAADLEAKFAEYDG Sbjct: 69 LRQDQFAYEEMRVLDVVMMGHVEMWAAMSERDAIYANPDATDDDYMKAADLEAKFAEYDG 128 Query: 121 YTAEARAGELLLGVGVAIEQHQGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDI 180 YTAEARAGELLLGVGV IEQH GPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDI Sbjct: 129 YTAEARAGELLLGVGVPIEQHNGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDI 188 Query: 181 NTIRWLEDMLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKVYPGNYDEYMLASSQAR 240 NTIRWLE++LNERNSTMIIISHDRHFLNQVCTHMADMDYGTLK+YPGNYD+YMLASSQAR Sbjct: 189 NTIRWLEEVLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKIYPGNYDDYMLASSQAR 248 Query: 241 AQQLSVNAKAKEKVAELQDFVRRFSANKSKARQATSRAKQIDKIKVEDIKPSSRANPFVR 300 AQQL+ NAKAKEKVAELQDFVRRFSANKSKARQATSRAKQIDKIKVEDIKPSSR NPF+R Sbjct: 249 AQQLAANAKAKEKVAELQDFVRRFSANKSKARQATSRAKQIDKIKVEDIKPSSRVNPFIR 308 Query: 301 FDGEKKLHRLAVETQGISKTYDRPIFKNFSIMVEAGEKIAIIGANGVGKTTLLRCTG 357 F+GEKKLHRLAVETQGI+KTYDR IFKNFSIMVEAGEKIAIIGANG GKTTLLR G Sbjct: 309 FEGEKKLHRLAVETQGITKTYDRDIFKNFSIMVEAGEKIAIIGANGAGKTTLLRSIG 365 Score = 79.3 bits (194), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 62/231 (26%), Positives = 105/231 (45%), Gaps = 38/231 (16%) Query: 6 KPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGD---LEPSAGNVSLDTNERLGKLR 62 + +F+N S+ G + +IGANG GK+T ++ +GGD L P G V N +G + Sbjct: 331 RDIFKNFSIMVEAGEKIAIIGANGAGKTTLLRSIGGDVTGLHPDGGLVKWAENANVGYMP 390 Query: 63 QDQFAYEEMRVLDVVMMGHTEMWAAMAERDAIYANPEATDDDYMKAADLEAKFAEYDGYT 122 QD TE +A T D+M E D Sbjct: 391 QDP----------------TEEFAG-----------GETLTDWMGKWTQEGD----DDQA 419 Query: 123 AEARAGELLLGVGVAIEQHQGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDINT 182 G LL G +++ +S G K R++ + + ++LL+DEPTN++D+ + Sbjct: 420 VRGILGRLLFS-GDDVKKSVKVLSG---GEKGRMMYGKLMLGRHNVLLMDEPTNHMDMES 475 Query: 183 IRWLEDMLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKVYPGNYDEYM 233 I L L + T+I +SHDR F++ + T + ++ G + + G Y++Y+ Sbjct: 476 IESLNIALEKYAGTLIFVSHDREFVSSLATRILEVKDGQVIDFQGGYEDYL 526 >gi|300311473|ref|YP_003775565.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 211 bits (538), Expect = 6e-56, Method: Compositional matrix adjust. Identities = 132/376 (35%), Positives = 201/376 (53%), Gaps = 30/376 (7%) Query: 1 MQFGPKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDTNERLGK 60 +Q G KPL EN+ + G++ GLIGANG GKS+ +L G+L GN+ R+ Sbjct: 9 LQRGVKPLLENVDLTLNPGDKIGLIGANGAGKSSLFAMLRGELHADQGNIDFPARWRMAY 68 Query: 61 LRQDQFAYEEMRVLDVVMMGHTEMWAAMAERDAIYANPEATDDDYMKA---ADLEAKFAE 117 + Q+ A + ++ + G A + + +A A EA DD A+L A+ Sbjct: 69 VAQETPALDR-PAIEYAIDGD----AHLRQLEAELARAEAQPDDKHDGNHIAELHTALAD 123 Query: 118 YDGYTAEARAGELLLGVGVAIEQHQGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNN 177 D YT +RA +LLLG+G ++EQ + P+++ + GW++R+ LAQAL D+LLLDEPTN+ Sbjct: 124 ADAYTVRSRAEQLLLGLGFSLEQMERPVASFSGGWRMRLNLAQALMCPSDLLLLDEPTNH 183 Query: 178 LDINTIRWLEDMLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKVYPGNYDEYMLASS 237 LD++ I WLED L T++IISHDR FL+ V + +D LK Y GNY + Sbjct: 184 LDLDAIIWLEDWLKRYEGTLLIISHDRDFLDGVVNVIVHIDDRKLKRYSGNYSAF----E 239 Query: 238 QARAQQLSVNA----KAKEKVAELQDFVRRFSANKSKARQATSRAKQIDKIK-------- 285 + R+ QL + A K + + A LQ F+ RF A +KARQA SR K + K++ Sbjct: 240 RQRSAQLELMAGMIEKQQRQRAHLQSFIDRFKAKATKARQAQSRMKALSKMEELAPLRAA 299 Query: 286 ----VEDIKPSSRANPFVRFDGEKKLHRLAVETQGISKTYDRPIFKNFSIMVEAGEKIAI 341 E +P S NP + EK +E + + D+ I ++ G++I + Sbjct: 300 AEFSFEFREPLSAPNPLLVM--EKVNAGYHIEGKNGAPDEDKIIVHGIDFSLQNGQRIGL 357 Query: 342 IGANGVGKTTLLRCTG 357 +G NG GK+TL++ Sbjct: 358 LGVNGAGKSTLIKTVA 373 Score = 86.3 bits (212), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 65/232 (28%), Positives = 101/232 (43%), Gaps = 46/232 (19%) Query: 6 KPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDTNERLGKLRQDQ 65 K + I +G R GL+G NG GKST +K + G+++P +G L +G Q Q Sbjct: 339 KIIVHGIDFSLQNGQRIGLLGVNGAGKSTLIKTVAGEIDPLSGTARLGKGLVIGYFAQHQ 398 Query: 66 FAYEEMRVLDVVMMGHTE--MWAAMAERDAIYANPEATDDDYMKAADLEAKFAEYDGYTA 123 V M+ H E +W + A D + + F ++G A Sbjct: 399 ----------VEMLRHDESPLW---------HLTRLAPDVREQELRNFLGSF-NFNGTMA 438 Query: 124 EARAGELLLGVGVAIEQHQGPMSNVAP---GWKLRVLLAQALFSNPDILLLDEPTNNLDI 180 S++AP G K R+ LA ++ P++LLLDEPTN+LD+ Sbjct: 439 ---------------------TSSIAPFSGGEKARLALALIVWQRPNLLLLDEPTNHLDL 477 Query: 181 NTIRWLEDMLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKVYPGNYDEY 232 T L L + T++++SHDRH L + G L+ + G+ D+Y Sbjct: 478 ETREALTMALAQFEGTLVLVSHDRHLLRATTDQFLIVAEGKLQPFDGDLDDY 529 >gi|300310722|ref|YP_003774814.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 640 Score = 167 bits (424), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 111/377 (29%), Positives = 196/377 (51%), Gaps = 19/377 (5%) Query: 1 MQFGPKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDTNERLGK 60 + FG L ++ G R GLIG NG GKS+ +KI+ G + G + + N ++ Sbjct: 11 LAFGHVALLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLLVMQQNLKIAY 70 Query: 61 LRQDQFAYEEMRVLDVVMMGHTEMWAAMAERDAIYANPEATDDDYM--KAADLEAKFAEY 118 + Q+ EEM V D V G E+ A +AE +AI +DD + + ++ Sbjct: 71 VDQEPHFPEEMTVFDAVASGLGELPALLAEYEAITGQFGGDNDDALLERMHQIQTVLDAA 130 Query: 119 DGYTAEARAGELLLGVGVAIEQHQGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNL 178 D ++ + L + + + G +S G K RV LA L NPD+LLLDEPTN+L Sbjct: 131 DAWSIGNKVETTLDKLNLNRDARIGELSG---GMKKRVALACGLVGNPDVLLLDEPTNHL 187 Query: 179 DINTIRWLEDMLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKVYPGNYDEYMLASSQ 238 D +I WLE +L + +++ I+HDR FL+ V T + ++D G + +PGN+ Y ++ Sbjct: 188 DFGSILWLEGLLRDFKGSILFITHDRSFLDNVSTRIIELDRGKILSFPGNFTTYQTRKAE 247 Query: 239 ARAQQLSVNAKAKEKVAELQDFVRRFSANKSKARQATSRAKQIDKIKVEDIKPSSRANPF 298 A + NAK + +A+ + ++R+ +++ + R ++++ ++V+ + R Sbjct: 248 LLANEEVENAKFDKFLAQEEVWIRK--GVQARRTRDEGRVRRLEALRVQRSERRERQGQ- 304 Query: 299 VRFD---GEKKLHRLAVETQGISKTY-DRPIFKNFSIMVEAGEKIAIIGANGVGKTTLLR 354 V+ + GE+ ++ E + ++K Y + I ++FS ++ G+K+ +IG NG GKTTLL+ Sbjct: 305 VKLEVGTGERS-GKIVAELENVNKAYGPKTIVRDFSTILMRGDKVGLIGQNGAGKTTLLK 363 Query: 355 CTGQRSGQVGRRRPATG 371 +G P +G Sbjct: 364 LI------LGEEAPDSG 374 Score = 86.7 bits (213), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 71/233 (30%), Positives = 115/233 (49%), Gaps = 38/233 (16%) Query: 3 FGPKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDTNERLGKLR 62 +GPK + + S G++ GLIG NG GK+T +K++ G+ P +G V T Sbjct: 329 YGPKTIVRDFSTILMRGDKVGLIGQNGAGKTTLLKLILGEEAPDSGTVKQGT-------- 380 Query: 63 QDQFAY-EEMRVLDVVMMGHTEMWAAMAERDAIYANPEATDDDYMKAADLEAKFAEYDGY 121 + Q AY ++MR A + A+ A D+++ Y Sbjct: 381 KMQVAYFDQMR--------------AQLNEETTLADTIAPGSDWVEVNGQRKHVMTY--- 423 Query: 122 TAEARAGELLLGVGVAIEQHQGPMSNVAPGWKLRVLLAQALFSNP-DILLLDEPTNNLDI 180 L A E+ + P+ ++ G + R+LLA+ LF+ P ++L+LDEPTN+LDI Sbjct: 424 ---------LSDFLFAPERARSPVRTLSGGERNRLLLAR-LFAKPANVLVLDEPTNDLDI 473 Query: 181 NTIRWLEDMLNERNSTMIIISHDRHFLNQVCTH-MADMDYGTLKVYPGNYDEY 232 +T+ LE++L E T+ ++SHDR FL+ V T +A G + Y G Y ++ Sbjct: 474 DTLELLEELLEEYEGTVFLVSHDRMFLDNVVTQVIASEGNGVWREYVGGYSDW 526 >gi|300313989|ref|YP_003778081.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 555 Score = 148 bits (374), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 113/372 (30%), Positives = 184/372 (49%), Gaps = 20/372 (5%) Query: 8 LFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDTNERLGKLRQDQFA 67 + ++IS+ F G + G++G NG GKST +KI+ G + G N +G L Q+ Sbjct: 21 ILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEGEAIPMPNLNIGYLPQEPQL 80 Query: 68 YEEMRVLDVVMMGHTEMWAAMAERDAIYANPEATDDDY----MKAADLEAKFAEYDGYTA 123 E V V E++ A A+ DA+YA D D+ + A LEA A DG + Sbjct: 81 DAEKTVRQEVESALGEVFEAQAKLDAVYAAYAEEDADFDALATEQARLEAIIAAADGNSL 140 Query: 124 EARAGELLLGVGVAIEQHQGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDINTI 183 + + + + + ++ G K RV L + L S PD+LLLDEPTN+LD ++ Sbjct: 141 NQQLE--MAADALRLPPWDAKIGVLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESV 198 Query: 184 RWLEDMLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKVYPGNYDEYMLASSQARAQQ 243 WLE L T++ I+HDR+FL+ + ++D G + GNY + L +AR +Q Sbjct: 199 DWLEQFLQRFPGTVVAITHDRYFLDNAAEWILELDRGHGIPWKGNYSSW-LEQKEARLKQ 257 Query: 244 LSVNAKAKEK-VAELQDFVRRFSANKSKARQATSRAKQIDKIKVEDIKPSSR-ANPFVRF 301 + A++K +A+ ++VR+ K RQA S+A+ ++ + + R + Sbjct: 258 EEASESARQKTIAKELEWVRQ----NPKGRQAKSKARLARFNELSEYEYQKRNETQEIFI 313 Query: 302 DGEKKLHRLAVETQGISKTY-DRPIFKNFSIMVEAGEKIAIIGANGVGKTTLLRCTGQRS 360 ++L +E + +SK Y DR + + + + AG + IIG NG GK+TL R Sbjct: 314 PVAERLGNEVIEFKNVSKGYGDRLLIDDLNFKIPAGAIVGIIGPNGAGKSTLFRMLA--- 370 Query: 361 GQVGRRRPATGQ 372 GR P +G+ Sbjct: 371 ---GRETPDSGE 379 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 66/231 (28%), Positives = 111/231 (48%), Gaps = 34/231 (14%) Query: 3 FGPKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDTNERLGKLR 62 +G + L ++++ K G G+IG NG GKST ++L G P +G V L ++ + Sbjct: 333 YGDRLLIDDLNFKIPAGAIVGIIGPNGAGKSTLFRMLAGRETPDSGEVVLGPTVKVSLVD 392 Query: 63 QDQFAYEEMRVLDVVMMGHTEMWAAMAERDAIYANPEATDDDYMKAADLEAKFAEYDGYT 122 Q + E++ N + +D AD+ ++ Sbjct: 393 QSR---EDL------------------------GNKKTVFEDVSGGADI-LTVGRFE-MP 423 Query: 123 AEARAGELLLGVGVAIEQHQGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDINT 182 + A G G Q + N++ G + R+ LA+ L ++LLLDEP+N+LD+ T Sbjct: 424 SRAYLGRFNFKGG----DQQKIVGNLSGGERGRLHLAKTLLQGGNVLLLDEPSNDLDVET 479 Query: 183 IRWLEDMLNERNSTMIIISHDRHFLNQVCTHMADMDYGT-LKVYPGNYDEY 232 +R LED L E T+++ISHDR FL+++ TH+ + + + + GNY EY Sbjct: 480 LRALEDALLEFAGTVLVISHDRWFLDRIATHIIAFEGDSQVTFFDGNYQEY 530 >gi|300311361|ref|YP_003775453.1| antimicrobial peptide ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 657 Score = 76.6 bits (187), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 62/209 (29%), Positives = 109/209 (52%), Gaps = 25/209 (11%) Query: 8 LFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDTNERLGKLRQDQFA 67 + ++++ G ++GA+G GKST M ILG +PSAG+ L GKL D+ A Sbjct: 24 VLNDVNLDIHAGELVAIVGASGSGKSTLMNILGCLDKPSAGSY-LVAGRETGKLDADELA 82 Query: 68 YEEMRVLDVVMMGHTEMWAAMAERDAIYANPEATDDDYMKAADLEAKFAEYDGYTAEARA 127 ++R E + + +R + ++ +AT + ++ + +A + RA Sbjct: 83 --QLR---------REHFGFIFQRYHLLSDLDATAN-----VEVPSVYAGLEPLQRRQRA 126 Query: 128 GELLLGVGVAIEQHQGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDINTIRWLE 187 GELL +G+A H P ++ G + RV +A+AL + D++L DEPT LD ++ + + Sbjct: 127 GELLARLGLAERSHHKP-GQLSGGQQQRVSIARALMNGGDVILADEPTGALDSHSGQEVI 185 Query: 188 DMLNERNS---TMIIISHDRHFLNQVCTH 213 +L E ++ T+II++HD QV +H Sbjct: 186 KILKELHAEGHTIIIVTHDM----QVASH 210 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,239,030 Number of extensions: 52399 Number of successful extensions: 636 Number of sequences better than 1.0e-08: 35 Number of HSP's gapped: 639 Number of HSP's successfully gapped: 40 Length of query: 397 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 310 Effective length of database: 1,207,925 Effective search space: 374456750 Effective search space used: 374456750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)