BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0041.p (788 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311510|ref|YP_003775602.1| phosphoenolpyruvate synthase/py... 1208 0.0 gi|300309436|ref|YP_003773528.1| phosphoenolpyruvate-protein pho... 83 8e-17 >gi|300311510|ref|YP_003775602.1| phosphoenolpyruvate synthase/pyruvate phosphate dikinase [Herbaspirillum seropedicae SmR1] Length = 791 Score = 1208 bits (3126), Expect = 0.0, Method: Compositional matrix adjust. Identities = 586/620 (94%), Positives = 605/620 (97%) Query: 1 MTDVESVGGKNASLGEMISQLAGAGVRVPGGFATTAQAFRDFLEHSANGGATLAQRIADR 60 MTDVESVGGKNASLGEMISQLAGAGVRVPGGFATTAQAFRDFLEHSA+GG TLA RIA R Sbjct: 21 MTDVESVGGKNASLGEMISQLAGAGVRVPGGFATTAQAFRDFLEHSADGGPTLAARIAKR 80 Query: 61 LVTLDIEDVRALAQAGAEIRQWIIDTPFQPALEQQIKSFYDKLVADSTAEMSFAVRSSAT 120 L LDI+DVR+LAQAGAEIRQWIIDTPFQP L+Q+I++FYDKLVADSTAEMSFAVRSSAT Sbjct: 81 LEGLDIDDVRSLAQAGAEIRQWIIDTPFQPRLDQEIRAFYDKLVADSTAEMSFAVRSSAT 140 Query: 121 AEDLPDASFAGQQETFLNVVGIDNVLEAMKHVFASLYNDRAISYRVHKGFTHAEVALSAG 180 AEDLPDASFAGQQETFLNVVGIDNVLEAMKHVFASLYNDRAISYRVHKGFTHAEVALSAG Sbjct: 141 AEDLPDASFAGQQETFLNVVGIDNVLEAMKHVFASLYNDRAISYRVHKGFTHAEVALSAG 200 Query: 181 VQRMVRSDVGAAGVMFTIDTESGFKDVVFITSSYGLGETVVQGAVNPDEFYVHKPMLEQG 240 VQRMVRSDVGAAGVMFTIDTESGFKDVVFITSSYGLGETVVQGAVNPDE+YVHKPMLE G Sbjct: 201 VQRMVRSDVGAAGVMFTIDTESGFKDVVFITSSYGLGETVVQGAVNPDEYYVHKPMLELG 260 Query: 241 KLPIIRRNIGSKLIKMEFTGEAKAGRSVKTVDVPVEMRNRYSLNDAEIVELAKYATIIEK 300 K P+IRRN+GSKLIKMEFTGEAKAGRSVKTVDVPVE+RNRYSL DAE+VELAKYATIIEK Sbjct: 261 KQPVIRRNLGSKLIKMEFTGEAKAGRSVKTVDVPVELRNRYSLTDAEVVELAKYATIIEK 320 Query: 301 HYERPMDIEWGKDGRDGKLYILQARPETVKSQQKATDAQQRFKLKGSSTVLATGRAIGQK 360 HY+RPMDIEWGKDGRDGKLYILQARPETVKSQQKATDAQQRFKLK S TVLATGRAIGQK Sbjct: 321 HYQRPMDIEWGKDGRDGKLYILQARPETVKSQQKATDAQQRFKLKSSGTVLATGRAIGQK 380 Query: 361 IGAGPVRVIRDAAEMERVQPGDVLVADMTDPNWEPVMKRAAAIVTNRGGRTCHAAIIARE 420 IGAGPVRVI D AEMERVQPGDVLVADMTDPNWEPVMKRA+AIVTNRGGRTCHAAIIARE Sbjct: 381 IGAGPVRVITDPAEMERVQPGDVLVADMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARE 440 Query: 421 LGVPAVVGCGDATDVLKDGTLVTVSCAEGDEGKIYDGLLETEITEVARGELPSIPLKIMM 480 LGVPAVVGCGDATDVLKDGTLVTVSCAEGDEGKIYDGLLETE+TEVARGELP IPLK+MM Sbjct: 441 LGVPAVVGCGDATDVLKDGTLVTVSCAEGDEGKIYDGLLETEVTEVARGELPPIPLKVMM 500 Query: 481 NVGNPQLAFDFQSIPNGGVGLARLEFIINNNIGVHPRAILEYPNIDADLKKAVESVARGH 540 NVGNPQLAFDFQSIPNGGVGLARLEFIINNNIGVHP+AILEYPNIDADLKKAVESVARGH Sbjct: 501 NVGNPQLAFDFQSIPNGGVGLARLEFIINNNIGVHPKAILEYPNIDADLKKAVESVARGH 560 Query: 541 ASPKAFYVDKLAEGIATIAAAFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFR 600 ASPKAFYVDKLAEGIATIAAAFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFR Sbjct: 561 ASPKAFYVDKLAEGIATIAAAFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFR 620 Query: 601 GAARYLSADFAEAFEMECQA 620 GAARYL+ DFAEAF+MECQA Sbjct: 621 GAARYLADDFAEAFQMECQA 640 >gi|300309436|ref|YP_003773528.1| phosphoenolpyruvate-protein phosphotransferase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 595 Score = 83.2 bits (204), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 99/416 (23%), Positives = 181/416 (43%), Gaps = 81/416 (19%) Query: 401 AAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDVLKDGTLVTVSCAEGDEGKI------ 454 A VT+ GG H AI+AR L +PA VG +A+ ++K ++ + +GD G + Sbjct: 183 AGFVTDLGGPNSHTAIVARSLAIPAAVGMHNASVLVKHDDVLII---DGDAGVVIVDPSP 239 Query: 455 -----YDGLLETEITEVAR----GELPSI-----PLKIMMNV---GNPQLAFDFQSIPNG 497 Y E + E + + P+I P+ ++ N+ G+ AF+ ++ Sbjct: 240 LVLAQYRARQERLLRERKKLSRLKKTPAITQDGEPITLLANIELPGDAVTAFEAGAM--- 296 Query: 498 GVGLARLEFIINNNIGVHPRAILEYPNIDADLKKAVESVARGHASPKAFYVDKLAEGIAT 557 G+GL R EF+ G + ++P+ D + ++V Sbjct: 297 GIGLFRSEFLFMGRAG----HLEKFPSEDEQFEHYKQAVI-------------------- 332 Query: 558 IAAAFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEE---NPMLGFRGAARYLSADFAEAF 614 A + V +R D +++ + DEE NP LG R A RY ++ + F Sbjct: 333 ---AMKGRSVTIRTLDVGADK--------PLDEDEETALNPALGLR-AIRYCLSE-PQMF 379 Query: 615 EMECQAMKRVRNDMGLTNVEIMVPFVRTLGQAEKVIGLL--GKNGLK----RGENGLRVI 668 + +A+ R ++++VP + + ++ + ++ K L+ + + + V Sbjct: 380 LTQLRAILRAS---AFGPIKLLVPMMAHAFEIDQTLAMIDQAKQQLRERKQKFDEEIPVG 436 Query: 669 MMCEVPSNAILAEEFLEHFDGFSIGSNDLTQLTLGLDRDSGMELLAADFDERDPALQALL 728 M E+P+ A+ F++ D SIG+NDL Q TL +DR +A +D PA+ LL Sbjct: 437 AMIEIPAAALALPLFVKRLDFLSIGTNDLIQYTLAIDRVD--HEVAHLYDPLHPAVLFLL 494 Query: 729 TKVISTCLAKGKYVGICGQGPSDHPDFAEWLMKQGIESISLNPDSVIDTWQKLAAS 784 + VIS G V +CG+ D + L+ G+ S++P ++ ++ S Sbjct: 495 STVISQGRKAGIPVSVCGEMAGDL-KWTRLLLGMGLLEFSMHPSQILSVKNEILNS 549 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,495,969 Number of extensions: 110420 Number of successful extensions: 334 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 335 Number of HSP's successfully gapped: 2 Length of query: 788 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 695 Effective length of database: 1,179,515 Effective search space: 819762925 Effective search space used: 819762925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)