BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0020.p (156 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311776|ref|YP_003775868.1| ubiquinol oxidase subunit IV pr... 161 2e-41 >gi|300311776|ref|YP_003775868.1| ubiquinol oxidase subunit IV protein [Herbaspirillum seropedicae SmR1] Length = 158 Score = 161 bits (408), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 84/126 (66%), Positives = 97/126 (76%), Gaps = 4/126 (3%) Query: 35 SLKSYTIGFVLAVILTAIPFWLVMGNVIEKSSTAGFVLLGLAVVQIVVHMIYFLHMNSRS 94 ++K Y GFVLAVILTAIPFWLVM I S+T G VLL LA VQIVVH+ YFLHMN +S Sbjct: 33 TMKDYVTGFVLAVILTAIPFWLVMNKSISSSATMGIVLLALAAVQIVVHIKYFLHMNGKS 92 Query: 95 EGGWTILALIFTIMVVVIMLAGSLWVMYHLNHNMMPGMS-QDLIQEAVPGS---MQNMQH 150 EGGW +LA+IFTI++VVI LAGSLWVMYHLNHNMMP MS +++ E VP S MQ MQH Sbjct: 93 EGGWNMLAMIFTIVIVVITLAGSLWVMYHLNHNMMPTMSPENMPTETVPQSMQGMQGMQH 152 Query: 151 NMPGMK 156 + P K Sbjct: 153 SAPQSK 158 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.136 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 498,213 Number of extensions: 17479 Number of successful extensions: 119 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 119 Number of HSP's successfully gapped: 1 Length of query: 156 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 78 Effective length of database: 1,250,540 Effective search space: 97542120 Effective search space used: 97542120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)