BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0015.p (453 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311780|ref|YP_003775872.1| major facilitator superfamily p... 650 0.0 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 151 1e-37 gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 139 5e-34 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 124 1e-29 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 119 4e-28 >gi|300311780|ref|YP_003775872.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 650 bits (1678), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/451 (73%), Positives = 392/451 (86%), Gaps = 6/451 (1%) Query: 3 MASTHIHHPEVPGKFSANTPVGGSDNVNNAKSDHPVIAPGDIAIGVVIGRASEYFDFFVY 62 MAST IH+ + G+ +G + DH I PG+IA+GVVIGRASEYFDFFVY Sbjct: 1 MASTSIHNGQGSGE------LGPHSTHPSTSHDHGKIEPGEIAVGVVIGRASEYFDFFVY 54 Query: 63 AIASVLVFPTIFFPFEQRLEGTLYSFTIFAFAFLARPFGTILFMAIQRKLGREVKLTLAL 122 A+ASVLVFP +FFP E +LEGTLY+F IFAFAFLARP GT +FMAIQ+ GREVKLTLAL Sbjct: 55 ALASVLVFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLAL 114 Query: 123 FLLGLSTAGIAFLPTYEHLGFTAIVLLSVLRIGQGLALGGSWDGLPSLLALNVPKNKRGW 182 F+LG STAGIA LPT+EH+G AI+LLS+LR+GQGLALGG+WDGLPSLLA+N P++KRGW Sbjct: 115 FMLGFSTAGIALLPTFEHIGVWAIILLSLLRVGQGLALGGAWDGLPSLLAMNAPQHKRGW 174 Query: 183 YAMLGQLGAPVGFIVAGLVFAYLIGNLSSDDFLEWGWRYPFYVAFAINVVALFARLRLVA 242 YA LGQLGAP+GF+VA +FAY++ +L++ DFLEWGWRYPFYVAFAINVVALF+RLRLV+ Sbjct: 175 YAALGQLGAPLGFVVAASLFAYMLSSLTTADFLEWGWRYPFYVAFAINVVALFSRLRLVS 234 Query: 243 SDEYAQLLKERELEPTGVVDITRTQGRNLIIGALAALASYALFHLVSVFPLSWISLYSMR 302 + EYA+LL ERELEP GVV++ TQGRN+IIGALAALASYALFHLV+VFPLSWI LYS+R Sbjct: 235 APEYAKLLDERELEPAGVVELATTQGRNIIIGALAALASYALFHLVTVFPLSWIKLYSLR 294 Query: 303 SMSDFLMVQVFGAVLMMVGIVLSGPIADRFGRRTTLGALAVLIAVLSGFVPTLINGGDTG 362 +DFL+VQ++G ++M VGIV+SG IADRFGRR TLG++A+LIA+LS FVPTL+NGG+ G Sbjct: 295 EATDFLVVQIWGGLIMAVGIVISGLIADRFGRRNTLGSMALLIAILSAFVPTLMNGGEAG 354 Query: 363 QDIFILVGFALLGLSYGQAAGAVTANFPAKYRYTGAALTSDLAWLVGAGFAPLVALGLSA 422 Q+ FIL+GFALLGLSYGQAAGAVTANF A+YRYTGAALTSDLAWLVGAGFAPLVALGLSA Sbjct: 355 QNTFILIGFALLGLSYGQAAGAVTANFKARYRYTGAALTSDLAWLVGAGFAPLVALGLSA 414 Query: 423 HFGLAYVSIYLLSGAVCSLAALSVNRALDIR 453 HFG+AYVS YLLSGAV SLAALS+NRAL+ R Sbjct: 415 HFGMAYVSFYLLSGAVGSLAALSLNRALEAR 445 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 151 bits (382), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 107/408 (26%), Positives = 190/408 (46%), Gaps = 7/408 (1%) Query: 36 HPVIAPGDIAIGVVIGRASEYFDFFVYAIASVLVFPTIFFPFEQRLEGTLYSFTIFAFAF 95 P + I ++G A E++DF VY S ++ +FFP + L + F F Sbjct: 2 QPQLNKQRQVIAAIVGNALEWYDFIVYGFFSAII-ARLFFPADNEYTSLLVALATFGIGF 60 Query: 96 LARPFGTILFMAIQRKLGREVKLTLALFLLGLSTAGIAFLPTYEHLGFTAIVLLSVLRIG 155 RP G +L + GR+ + + + L+ L+ A IAF PTY +G A +++ V R+ Sbjct: 61 FMRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARML 120 Query: 156 QGLALGGSWDGLPSLLALNVPKNKRGWYAMLGQLGAPVGFIVAGLVFAYLIGNLSSDDFL 215 QG A GG + + L + P +KRG Y +G + + A + NLS + Sbjct: 121 QGFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALD 180 Query: 216 EWGWRYPFYVAFAINVVALFARLRLVASDEYAQLLKERELEPTGVVDITRTQGRNLIIGA 275 WGWR PF + I V L+ R + +D++ + + P ++D+ +T R +++ Sbjct: 181 SWGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSM 240 Query: 276 LAALASYALFHLVSVFPLSWISLYSMRSMSDFLMVQVFGAVLMMVGIVLSGPIADRFGRR 335 + F++V V ++ + + MVQ+ L+ I +G ++DR GRR Sbjct: 241 GQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRR 300 Query: 336 TTL--GALAVLIAVLSGFVPTLINGGDTGQDIFIL--VGFALLGLSYGQAAGAVTANFPA 391 T L +LA L+ V FV ++ + + ++ + ++G ++G A++ FP Sbjct: 301 TVLLYSSLAFLLMVYPLFV--WVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPT 358 Query: 392 KYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFGLAYVSIYLLSGAVC 439 + R TG A+ +LA ++ GFAP + L+ G + + A C Sbjct: 359 RVRSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAAC 406 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 139 bits (350), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 117/418 (27%), Positives = 195/418 (46%), Gaps = 23/418 (5%) Query: 50 IGRASEYFDFFVYAIASVLVFPTIFFPFEQRLEGTLYSFTIFAFAFLARPFGTILFMAIQ 109 IG A EY+DFF+YA A+ L+FP +FFP + S + ++ARP G + + Sbjct: 22 IGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVARPIGAFVLGHLG 81 Query: 110 RKLGREVKLTLALFLLGLSTAGIAFLPTYEHLGFTAIVLLSVLRIGQGLALGGSWDGLPS 169 GR+ L +FL+G ST G+ LPTY+ +G A LL ++R+ QG A+ G G S Sbjct: 82 DTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRLAQGFAVAGEISGASS 141 Query: 170 LLALNVPKNKRGWYAMLGQLGAPVGFIVAGLVFAYLIGNLSSDDFLEWGWRYPFYVAFAI 229 ++ + P +RG+++ G G I+A VF L + ++ F WGWR PF ++F + Sbjct: 142 MILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMPAEQFNVWGWRIPFLLSFLV 201 Query: 230 NVVALFARLRLVASDEYAQLLKERELEPTGVVDITRTQGRNLIIGALAALASYALFHLVS 289 + R + + + KE + VVD R N++ + AL +++ Sbjct: 202 IIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCSPNML-----RVVCMALMNVIP 256 Query: 290 VFPLSWISLYSMR-------SMSDFLMVQVFGAVLMMVGIVLSGPIADRFGRRTTLGALA 342 V + + Y+++ + +L + V G ++ ++ I G ++DR GRR + + Sbjct: 257 VVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIGRRPPI-IVG 315 Query: 343 VLIAVLSGFVPTLINGGDTGQDIFILVGFALLGLSYGQAAGAVTAN-----FPAKYRYTG 397 L+A L F L ++ ++ + G+ Y Q AV + FPA+ R + Sbjct: 316 SLLAGLFAFA-YLYAISIRNVELAFVMSILMWGVVY-QGYNAVFPSFYPELFPARARVSA 373 Query: 398 AALTSDLAWLVGAGFAPL---VALGLSAHFGLAYVSIYLLSGAVCSLAALSVNRALDI 452 A+ +L + A L VA S++ L SI V +LAALS I Sbjct: 374 MAIAQNLGTAITAMLPALFTAVAPPGSSNIWLTVGSIAFAITIVSALAALSARETYRI 431 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 124 bits (312), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 107/399 (26%), Positives = 182/399 (45%), Gaps = 15/399 (3%) Query: 18 SANTPVGGSDNVNNAKSDHPVIAPGDIAIGVVIGRASEYFDFFVYAIASVLVFPTIFFPF 77 ++ P G +V+N + ++A VIG E+FDF VY+ +V++ +FFP Sbjct: 3 ASTLPADGIVSVSNQQRRRAIVA-------TVIGNGLEWFDFTVYSFFAVII-AKLFFPT 54 Query: 78 EQRLEGTLYSFTIFAFAFLARPFGTILFMAIQRKLGREVKLTLALFLLGLSTAGIAFLPT 137 L L + F F RP G IL ++GR+ L++ + L+ + T I PT Sbjct: 55 GNDLTSLLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPT 114 Query: 138 YEHLGFTAIVLLSVLRIGQGLALGGSWDGLPSLLALNVPKNKRGWYAMLGQLGAPVGFIV 197 YE +G A +++ V R+ QG + GG + L P+ +R +Y+ Q V ++ Sbjct: 115 YESIGLFAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLL 174 Query: 198 AGLVFAYLIGNLSSDDFLEWGWRYPFYVAFAINVVALFARLRLVASDEYAQLLKERELEP 257 V ++ +L + WGWR PF + I V + R L +E L+E++ Sbjct: 175 GAAVGTFVTASLDAAALASWGWRLPFLLGIIIGPVGYYIRHHL---EETPTFLEEKDKTD 231 Query: 258 TGVVDITRTQGRNLIIG-ALAALASYALFHLVSVFPLSWISLYSMRSMSDFLMVQVFGAV 316 + + ++ R R ++ L + + L+ P + + + + F+ V G Sbjct: 232 SPLKEVIRNFPRETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLC 291 Query: 317 LMMVGIVLSGPIADRFGRRTTLGALAVLIAVLSGFVPTLINGGDTGQD--IFILVGFALL 374 +M++ ++ G +ADR GRR L A LI +L+ + IN + IF LV L+ Sbjct: 292 IMVLAPIV-GRLADRIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLI 350 Query: 375 GLSYGQAAGAVTANFPAKYRYTGAALTSDLAWLVGAGFA 413 G A + FPAK TG ++ +LA + GFA Sbjct: 351 AAYTGPILAAFSELFPAKVLSTGLSVAYNLAVTIFGGFA 389 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 119 bits (299), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 122/441 (27%), Positives = 203/441 (46%), Gaps = 20/441 (4%) Query: 18 SANTPVGGSDNVNNAKSDHPVIAPGDIAIGVVIGRASEYFDFFVYAIASVLVFPTIFFPF 77 S+NT G+D + + ++ +I+ IG A E+FD +Y A +V FFP Sbjct: 2 SSNTAAPGAD-ITHPQARRAIISSS-------IGNALEWFDILIYG-AFAVVIAKQFFPT 52 Query: 78 EQRLEGTLYSFTIFAFAFLARPFGTILFMAIQRKLGREVKLTLALFLLGLSTAGIAFLPT 137 L +F F +F RP G ++ A + GR+ L L++ L+ + TA IAF+P+ Sbjct: 53 GDDSVSLLLTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPS 112 Query: 138 YEHLGFTAIVLLSVLRIGQGLALGGSWDGLPSLLALNVPKNKRGWYAMLGQLGAPVGFIV 197 Y +G A +++ ++ QG + GG + + L + P ++RG+++ + ++ Sbjct: 113 YASIGLLAPAGIALGKMIQGFSAGGEFGSSTAFLVEHAP-HRRGFFSSWQVASQGISLLL 171 Query: 198 AGLVFAYLIGNLSSDDFLEWGWRYPFYVAFAINVVALFARLRLVASDEYAQLLKERELEP 257 A L A L L+ + WGWR PF I ++ R L DE + + E Sbjct: 172 AALFGAVLNNMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHL---DEAPEFKESSEKTN 228 Query: 258 TGVVDITRTQGRNLIIGALAALASYALFHLVSVFPLSWISLYSMRSMSDFLMVQVFGAVL 317 + D Q L+IGA + + + +L P + + + S F + V G ++ Sbjct: 229 APLRDTFAHQKMRLLIGAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAG-LI 287 Query: 318 MMVGIVLSGPIADRFGRRTTLGALAVLIAVLS--GFVPTLINGGDTGQDIFILVGFALLG 375 M +G L G ++D+ GR + + L VL+ FV L N Q + + +L Sbjct: 288 MFIGSPLVGALSDKIGRTPFMITSSALYIVLTYPMFV-FLTNSPGFLQLLLLQTVIGVLM 346 Query: 376 LSYGQAAGAVTAN-FPAKYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFGLAY-VSIYL 433 Y A A+ A+ FP R TG +L ++A + GFA L+ L G VS ++ Sbjct: 347 TMYFAAMPALLADIFPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFV 406 Query: 434 LSGAVCSL-AALSVNRALDIR 453 + GAV SL A+LS L +R Sbjct: 407 IFGAVLSLIASLSARLVLRLR 427 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.143 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,448,519 Number of extensions: 65181 Number of successful extensions: 380 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 352 Number of HSP's successfully gapped: 12 Length of query: 453 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 365 Effective length of database: 1,203,190 Effective search space: 439164350 Effective search space used: 439164350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)