BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0014.p (480 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 340 2e-94 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 262 5e-71 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 76 7e-15 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 66 7e-12 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 340 bits (871), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 185/403 (45%), Positives = 254/403 (63%), Gaps = 10/403 (2%) Query: 61 IAHYRQIAQTAERAKFDALFLADGVAIRSHDAET----LHLTSRAAT-FEPLTLLSALSQ 115 + +Y IA+T ER KFD LF AD +A+ + ++ L++ + AT +PL +L AL+ Sbjct: 32 LDYYLHIARTLERGKFDLLFFADRLAVSTRYGDSHLHGLYIGDQDATRLDPLPILGALAA 91 Query: 116 ATEHIGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTSWSESEAHNFNLDKHPEH 175 AT HIGL AT STTY++PY +AR+FA+LDHLS GRA WNVVTS ++ EA NF L + H Sbjct: 92 ATSHIGLGATRSTTYSQPYSLAREFATLDHLSGGRAAWNVVTSVNQGEADNFGLKETLPH 151 Query: 176 GARYERAEEFVDVVRGLWDSWEDDAFRFDQAEGVHFDHNKLHAIHHKGKHFSVRGPLNVA 235 RY+RA+EF++V LW SW+ DA R Q + D +K+ AI H+G HFSVRGPLN+ Sbjct: 152 DVRYDRADEFLEVTHKLWHSWQPDALRLQQDR--YADADKVAAIDHQGAHFSVRGPLNIP 209 Query: 236 RPVQGHPVVVQAGSSEPGQELAARTAEVIFTAQQSLEDAQHFYSSLKSRLARYGREPDEL 295 QGHPV++QAGSS+ GQ+ AAR AEV+F Q L Q FY LK+R ++GR P ++ Sbjct: 210 ASPQGHPVIIQAGSSDRGQDFAARWAEVVFNIQPDLPRLQAFYRDLKNRAQQFGRRPQDI 269 Query: 296 KIMPGVFPVVGRSESEALEKFEELQSLIHPTVGLSLLQQHLGGIDLSGYPLDGPLPENLQ 355 +I+ V P VG+S A E E+ +L P VGLS L H+ +D SG+ LD PL + Sbjct: 270 RILSAVMPFVGQSRQHARELQEQANALADPLVGLSTLSSHM-NVDFSGFALDAPLGD--V 326 Query: 356 EPNGTKSRFQLVTALARREQLTIRQLYLRVATARGHWSIHGTPESIVDRLEEWFLKGGAD 415 + +G + F+L+ A++ QLT+ A I GT + D L+ + GAD Sbjct: 327 QVSGIQGLFKLLRAISAERQLTLADAGRLYAQGVLTPQIAGTAADVADWLQSIIVANGAD 386 Query: 416 GFNIMPPTLPGGLDDFVELVLPELRRRGLFRSEYEGKTLRENL 458 GF I P LP G DDFV+ V+PEL+RRG FR +Y G+ LR++L Sbjct: 387 GFVISPSHLPYGFDDFVDQVIPELQRRGAFRQDYAGRQLRQHL 429 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 262 bits (669), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 161/422 (38%), Positives = 231/422 (54%), Gaps = 15/422 (3%) Query: 60 NIAHYRQIAQTAERAKFDALFLADGVAI----RSHDAETLHLTSRAATFEPLTLLSALSQ 115 +IA++ +A+ ER KFD LFLAD V + + + TL T + +PL L+ A++ Sbjct: 36 DIAYWTDLARLLERGKFDGLFLADIVGVYDVYQGNTDLTLRETIQLPVNDPLMLVPAMAA 95 Query: 116 ATEHIGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTSWSESEAHNFNLDKHPEH 175 TEH+G T + TY P+ AR+ ++LDHLS GR GWN+VT + +S A L + EH Sbjct: 96 VTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEH 155 Query: 176 GARYERAEEFVDVVRGLWD-SWEDDAFRFDQAEGVHFDHNKLHAIHHKGKHFSVRGPLNV 234 RY+RA+EF++VV LW+ SWED A D+A V+ D K+H I H G ++ V G +++ Sbjct: 156 DQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARRVYADPAKVHKIAHHGDYYHVEG-IHL 214 Query: 235 ARPV-QGHPVVVQAGSSEPGQELAARTAEVIFTAQQSLEDAQHFYSSLKSRLARYGREPD 293 + P Q PV+ QAGSS GQ AR AE +F + QS + + S++ R GR+PD Sbjct: 215 SEPSRQRTPVLYQAGSSGRGQAFGARHAECVFISSQSKDATRKLVQSIRDGAVREGRDPD 274 Query: 294 ELKIMPGVFPVVGRSESEALEKFEELQSLIHPTVGLSLLQQHLGGIDLSGYPLDGPLPEN 353 +KI G+ V GR+E +A E++ E P GL+ GID S Y LD P+ Sbjct: 275 AIKIFMGLTVVTGRTEQQAQERYREYARYASPEAGLAHYSAGT-GIDFSQYELDEPI--- 330 Query: 354 LQEPNGTKSRFQLVTALARRE-QLTIRQLYLRVATARGHWSIHGTPESIVDRLEEWFLKG 412 P T + L + + E T R+L ++ + SI G P+ + D LE W Sbjct: 331 --RPAKTNAIESLTKVVTQPELNWTKRKLLQQLELGGRYASIVGAPDRVADELESWVADT 388 Query: 413 GADGFNIMPPTLPGGLDDFVELVLPELRRRGLFRSEYEGKTLRENLGLARPAHRIYQQSG 472 G DG+N+ P +DFVELV+P L+ RG ++ EY TLRE L R A + G Sbjct: 389 GIDGYNLTRTVTPESFEDFVELVIPVLQERGSYKREYAQGTLREKL-FQRGATLPQEHVG 447 Query: 473 AG 474 AG Sbjct: 448 AG 449 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 75.9 bits (185), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 69/263 (26%), Positives = 119/263 (45%), Gaps = 39/263 (14%) Query: 60 NIAHYRQIAQTAERAKFDALFLADGVAIRSHDAETLHLTSRAATFEPLTLLSALSQATEH 119 ++ + +++A TAE+A F+ L+ + AE H E ++ AL AT Sbjct: 42 SLEYNQRLAVTAEKAGFEYA-LSQIRFTAGYGAEYQH--------ESVSFSQALLHATTK 92 Query: 120 IGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTSWSESEAHNFNLDKHP--EHGA 177 + ++A + P VA++ A++DH+SNGR NVV+ W + E F P EH Sbjct: 93 LKVLAAILPGPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGE---FQAIGEPWLEHDE 149 Query: 178 RYERAEEFVDVVRGLWDSWEDDAFRFDQAEGVHFDHNKLHAIHHKGKHFSVRGPLNVARP 237 RY R+ EF+ ++G+W D F F KG + +P Sbjct: 150 RYRRSNEFIRALKGIWTQ---DDFTF------------------KGDFYRFNNYTLSPKP 188 Query: 238 VQG-HPVVVQAGSSEPGQELAARTAEVIFTAQQSLEDAQHFYSSLKSRLARYGREPDELK 296 VQ HP + Q GSS ++ AA ++ FT +LE + ++++ A+ G +++ Sbjct: 189 VQKPHPEIFQGGSSRAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQNGH---KVR 245 Query: 297 IMPGVFPVVGRSESEALEKFEEL 319 I F + +E EA +++ Sbjct: 246 IGVNAFVIARDTEEEAQAVLQDI 268 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 65.9 bits (159), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 101/389 (25%), Positives = 159/389 (40%), Gaps = 76/389 (19%) Query: 60 NIAHYRQIAQTAERAKFDALFLADGVAIRSHDAETLHLTSRAATFEPLTLLSALSQATEH 119 + + RQIA T+E +D L + G A+ +P + ++L T+ Sbjct: 28 TLEYMRQIAVTSETLGYDGLLIPTG----------------ASCLDPWVVAASLVPVTQR 71 Query: 120 IGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTSW--SESEAHNFNLDKHPEHGA 177 I L+ + T+ P AR+ A+LD NGR NVV +E EA L +H Sbjct: 72 IKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGVFL----KHDE 127 Query: 178 RYERAEEFVDVVRGLWDSWEDDAFRFDQAEGVHFDHNKLHAIHHKGKHFSVR-GPLNVAR 236 RYE A+E++ + R L Q E V+++ GKHF +R G L Sbjct: 128 RYEAADEYLTIWRKLL-----------QGETVNYE----------GKHFRIRNGQLFHPA 166 Query: 237 PVQGHPVVVQAGSSEPGQELAARTAEVIFTAQQSLEDAQHFYSSLKSRLARYGREPDELK 296 Q +P + GSS +LAA+ + T + + ++ R AR GR ++ Sbjct: 167 VQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVRERAARQGR---TVR 223 Query: 297 IMPGVFPVVGRSESEALEKFEELQSLIHPTVGLSLLQQHLGGIDLSGYPLDGPLPENLQE 356 + +V + +EA E L S + ++ QQ+ +D G Sbjct: 224 FGVRLHAIVRETTAEAWTAAERLISKLTDE-DIARAQQNYARMDSVGQ------------ 270 Query: 357 PNGTKSRFQLVTAL--ARREQLTIR-QLYLRVATARG--HWSIHGTPESIVDRLEEWFLK 411 Q + AL RR++L I L+ V RG S+ G ++ +RL+E + Sbjct: 271 --------QRMAALHGGRRDKLEIAPNLWAGVGLVRGGAGTSLVGDATTVAERLQE-YAD 321 Query: 412 GGADGFNIMP-PTLPGGLDDFVELVLPEL 439 G D F + P L + F ELV P L Sbjct: 322 LGVDTFVLSGYPHLEEAI-RFAELVFPLL 349 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,655,372 Number of extensions: 72472 Number of successful extensions: 209 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 5 Length of query: 480 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 391 Effective length of database: 1,198,455 Effective search space: 468595905 Effective search space used: 468595905 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)