BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0257.0027.p
         (230 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen...    76   3e-15
gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen...    67   2e-12
gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas...    60   2e-10

>gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 288

 Score = 75.9 bits (185), Expect = 3e-15,   Method: Compositional matrix adjust.
 Identities = 52/171 (30%), Positives = 89/171 (52%), Gaps = 2/171 (1%)

Query: 15  GGILENCRSGQILIDCSTSEPMSSARIAEQLAAKGVSFVDAPLARTPVEAMQGRLNTMVG 74
           GG+L++   G +++D +T++P S+ R+A +L A G+  VDAP++  P  A    +  ++G
Sbjct: 79  GGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAPVSGGPKGAATATMGMVIG 138

Query: 75  ADDATFAKVKPVLEAFCENIFHIGGTTSAQKLKLLNNFIAIGTATL-ATEALAMCDAVGV 133
               T A+++PVL        H+G   +    KLLNN +  GT  L A EA  +  A G+
Sbjct: 139 GSAETVARIEPVLAVMSAKRVHVGPVGAGHVAKLLNNLVT-GTHLLIAGEAARIAAAAGL 197

Query: 134 DQRKFFDVISKGGANSGIFQMIAGGILEGRSDGMKFSIANAGKDLSYYNNM 184
           D R+ F  ++ G ANS + ++     +   +    F++    KDL    N+
Sbjct: 198 DARQIFQGLAAGSANSKVIEVFYPTWIFNEAFDSGFTMKLMRKDLRLAMNL 248


>gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 297

 Score = 66.6 bits (161), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 42/130 (32%), Positives = 71/130 (54%), Gaps = 2/130 (1%)

Query: 16  GILENCRSGQILIDCSTSEPMSSARIAEQLAAKGVSFVDAPLARTPVEAMQGRLNTMVGA 75
           G+L + + G +LIDCST  P ++ ++A    A+G   +DAP++     A  G L  MVG 
Sbjct: 81  GVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVSGGTNGATAGTLTFMVGG 140

Query: 76  DDATFAKVKPVLEAFCENIFHIGGTTSAQKLKLLNNFIAIGTATLAT-EALAMCDAVGVD 134
               F   +P L+   + I+H G + S Q +K+ NN + +G   + T EA+ +  A G+D
Sbjct: 141 SKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNML-LGILMIGTSEAIRLGMANGMD 199

Query: 135 QRKFFDVISK 144
            +   +++SK
Sbjct: 200 PKVLSEIMSK 209


>gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 298

 Score = 59.7 bits (143), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 37/148 (25%), Positives = 70/148 (47%), Gaps = 1/148 (0%)

Query: 16  GILENCRSGQILIDCSTSEPMSSARIAEQLAAKGVSFVDAPLARTPVEAMQGRLNTMVGA 75
           G +   + G ++I  +T  P  +  + ++L  KG+  +DAP++     A  G +  M   
Sbjct: 81  GAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDAPVSGGAARAASGEMTMMTSG 140

Query: 76  DDATFAKVKPVLEAFCENIFHIGGTTS-AQKLKLLNNFIAIGTATLATEALAMCDAVGVD 134
               +AK++ VL      ++ +G T     K+K++N  +A      + EA+A+    GV+
Sbjct: 141 PAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHIAASAEAMALGLREGVN 200

Query: 135 QRKFFDVISKGGANSGIFQMIAGGILEG 162
               ++VI+    NS +F+     IL G
Sbjct: 201 PDALYEVITHSAGNSWMFENRVPHILSG 228


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.131    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 643,176
Number of extensions: 24487
Number of successful extensions: 85
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 83
Number of HSP's successfully gapped: 4
Length of query: 230
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 148
Effective length of database: 1,231,600
Effective search space: 182276800
Effective search space used: 182276800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 129 (54.3 bits)