BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0257.0022.p (333 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane ... 231 4e-62 gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane ... 201 6e-53 gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane ... 138 6e-34 gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane ... 128 6e-31 gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [H... 100 1e-22 >gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 231 bits (590), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 130/314 (41%), Positives = 190/314 (60%), Gaps = 14/314 (4%) Query: 16 PLVKQQAIEWLLELQSETVTDDTRRNWQRWRQAHPEHELAWQKVEAFGDRMNTL----QP 71 P V ++A+EWLLELQ++ + T W+ WR AHP+HE AW+++E+ R+ L Sbjct: 18 PQVAERALEWLLELQTQPLAPSTLEAWEAWRSAHPDHERAWRRIESVRGRLQALASPVNA 77 Query: 72 SLARAALTAKDSLERRRAVKMVAILLFAGTAALSVEQILPWRTWRADHVTRAGEIRTVTL 131 +LA+AALT S RR+ +K +A+L+F G A + + PW+ WRAD T G+ R + L Sbjct: 78 ALAQAALT--PSGNRRQVIKTLALLVFGGGVAWGLTRSEPWQQWRADLRTDIGQQRRLVL 135 Query: 132 EDGSQLALNTRSTVKVEFSGKERRIRLMSGEIMISTAHENVTDATPYRPFLVETPDGELQ 191 EDGSQL LNT S V + FS +ERR+RL+SGE++++T A RPFLVET G Sbjct: 136 EDGSQLVLNTHSAVNLAFSAEERRLRLISGELLVTTGK-----AGGGRPFLVETAQGLAY 190 Query: 192 ALGTRFTVHKTEAERSSVAVYEGAVEVRP--ALGGTRVLSAGSALEFSTRDIGPLSVAPA 249 ALGTR+ V + A R+ V+V+EGAV + P A RVL AG FS + +G ++ A Sbjct: 191 ALGTRYAVRQ-RAGRTEVSVWEGAVRLVPRQAAQQARVLQAGQQAAFSAQGVGDVTTADE 249 Query: 250 HAAAWTRGMLVVEDMPLSDFVAELARYRSGWINCDDAIGQLKVTGTYPLADTDKVLLMLT 309 AWT G++V M L F+AEL RY + ++C A+ ++++G+YP+AD+D V+ L Sbjct: 250 TRMAWTDGVIVARGMRLDAFLAELGRYTTDRLSCAPAVSGVRISGSYPVADSDAVIATLA 309 Query: 310 RVFPVDIAYLTRYW 323 + R+W Sbjct: 310 AALGLQAQLRQRFW 323 >gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 201 bits (512), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 124/309 (40%), Positives = 168/309 (54%), Gaps = 6/309 (1%) Query: 21 QAIEWLLELQSETVTDDTRRNWQRWRQAHPEHELAWQKVEAFGDRMNTLQPSLARAALTA 80 +A WL+E+ + R +WRQ PEHE AW + + L P A AL Sbjct: 18 EAATWLMEMHEGPLDAARRSKLAQWRQRSPEHERAWSRAALLQETFAALPPGGA-TALKQ 76 Query: 81 KDSLERRRAVKMVAILLFAGTAALSVEQILPWRTWRADHVTRAGEIRTVTLEDGSQLALN 140 RR+A+K++A L G A L A T GE R V L+DGS+L LN Sbjct: 77 VGPAGRRKAIKLLAYALTLGPAGWLAYHALSGPEQGARIATAIGERREVQLDDGSRLWLN 136 Query: 141 TRSTVKVEFSGKERRIRLMSGEIMISTAHENVTDATPYRPFLVETPDGELQALGTRFTVH 200 T + + ++F+ +R +RL GEIM+ TA + P RPFLV T G L+ LGTRFTV Sbjct: 137 TDTRITLDFTPAQRLLRLQRGEIMVLTAPDR---QQPPRPFLVATRQGSLRPLGTRFTVR 193 Query: 201 KTEAERSSVAVYEGAVEVRPALGGTR-VLSAGSALEFSTRDIGPLSVAPAHAAAWTRGML 259 + + + VAV +GAVE+ PA + VL +G F+ AAW+RG+L Sbjct: 194 ERD-QAVEVAVLQGAVEITPASSAQQTVLPSGMQTRFTRSGSAAPRPCDDTQAAWSRGLL 252 Query: 260 VVEDMPLSDFVAELARYRSGWINCDDAIGQLKVTGTYPLADTDKVLLMLTRVFPVDIAYL 319 + E MPL+ F+ EL RYRSG + D AI L+V+G +PLADTD+VL +L + PV + L Sbjct: 253 IAERMPLAQFIGELGRYRSGLLQADPAIAGLEVSGVFPLADTDRVLRLLAQTLPVRVQQL 312 Query: 320 TRYWVTVRP 328 TRYWV V P Sbjct: 313 TRYWVRVLP 321 >gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 138 bits (348), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 115/345 (33%), Positives = 158/345 (45%), Gaps = 28/345 (8%) Query: 9 SRPVTLSPLVKQQAIEWLLELQS----ETVTDDTRRNWQRWRQAHPEHELAWQKVEAFGD 64 S P+ L V QAI WL++L++ V + + WR A P HE W ++ A + Sbjct: 3 SNPIPLQDPVLDQAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMASEE 62 Query: 65 RMNTLQPSLARAALTAKDSLERRRAVK---------MVAILLFAGTAALSVEQILPWRTW 115 + L +L A L+R + M +L G AL + Sbjct: 63 QFQRLV-ALPVPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAARQAGLF 121 Query: 116 RA----DHVTRAGEIRTVTLEDGSQLALNTRSTVKVEFSGKERRIRLMSGEIMISTAHEN 171 A + TR G+ R + L DG+ LALNT S+V++ F R + L GEI I T + Sbjct: 122 EALDGASYATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGAD- 180 Query: 172 VTDATPYRPFLVETPDGELQALGTRFTVHKTEAERSSVAVYEGAVEVRPALGGTRVLSAG 231 +RPF V+T LQALGTRF V + + + + V EG V V + G L A Sbjct: 181 -AQWPHHRPFWVQTAHARLQALGTRFDV-RQHGDGTQLTVLEGRVAVH--VAGVDPLVAQ 236 Query: 232 SALEFSTRDIGPLSVAPAHAAA-----WTRGMLVVEDMPLSDFVAELARYRSGWINCDDA 286 G L A A+A W LVV M L+D VA+LARYRSGW+ CD A Sbjct: 237 PGEIVDIHPDGRLVRRAARASAMDPTAWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPA 296 Query: 287 IGQLKVTGTYPLADTDKVLLMLTRVFPVDIAYLTRYWVTVRPRGN 331 + +V+G + L DTD L L + P+ I TR+WV V G Sbjct: 297 VADWQVSGVFQLQDTDVALEALAQALPLTIERRTRFWVRVAAAGK 341 >gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 128 bits (322), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 94/318 (29%), Positives = 150/318 (47%), Gaps = 15/318 (4%) Query: 18 VKQQAIEWLLELQSETVTDDTRRNWQRWRQAHPEHELAW----QKVEAFG-DRMNTLQPS 72 + +QAIEW+L+L+ + R +++W+ A P H +A+ Q V+ F R P Sbjct: 7 IVEQAIEWMLQLEQGDLPPGERAAFEQWKAADPRHAMAYANLSQSVQQFDVPRQVGAAPV 66 Query: 73 LARAALTAKDSLERRRAVKMVAILLFAGTAALSV-EQILPWRTWRADHVTRAGEIRTVTL 131 + R L K +RR+A+K +A L A ++ +++ P AD T G+ + TL Sbjct: 67 ILRNTLQQKS--KRRKALKRIAALAGLAVGAAALLDRVTPVAALTADLHTGTGQRQRTTL 124 Query: 132 EDGSQLALNTRSTVKVEFSGKERRIRLMSGEIMISTAHENVTDATPYRPFLVETPDGELQ 191 DGS + LN RS + S R++RL+ GEI + A N PF +ET G + Sbjct: 125 ADGSIITLNARSAIDHLISPVLRQVRLLEGEIQVQIAAANAL------PFSIETVHGSVS 178 Query: 192 ALGTRFTVHKTEAERSSVAVYEGAVEVRPALGGTRVLSAGSALEFSTRDIGPLSVAPAHA 251 A + + R+ + + A + G L+AG F +IG A Sbjct: 179 ATAGTLML-GLQPLRTDLVAQQAAALLSTRNGQHATLAAGQRTWFDQDEIGAPRAAGGAE 237 Query: 252 AAWTRGMLVVEDMPLSDFVAELARYRSGWINCDDAIGQLKVTGTYPLADTDKVLLMLTRV 311 +W G D L + VA L YR G + D A+ QL+++G +PL DTD+ L L Sbjct: 238 NSWVEGYYTASDNSLEEVVAALRPYRKGLLRLDPAVAQLRISGAFPLDDTDRALSALASA 297 Query: 312 FPVDIAYLTRYWVTVRPR 329 P+ + ++ YWV++ R Sbjct: 298 LPITVNRVSPYWVSISGR 315 >gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 100 bits (249), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 74/212 (34%), Positives = 104/212 (49%), Gaps = 13/212 (6%) Query: 108 QILPWRTWRADHVTRAGEIRTVTLEDGSQLALNTRSTVKVEFSGKERRIRLMSGEIMIST 167 Q P R AD T G TL DGS+L L+ S F + R + L+ GE+++ Sbjct: 118 QAYPARYLLADMRTATGHWDARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVEV 177 Query: 168 AHENVTDATPYRPFLVETPDGELQALGTRFTVHKTEAERSSVAVYEGAVEVRPALGGTR- 226 A + P RPF+++T DG ++ALGTRF V + A + V++ E V ++P T+ Sbjct: 178 ARD------PARPFVIQTADGSIRALGTRFLVRRDRAG-TLVSMLESKVSIQPGADTTQA 230 Query: 227 --VLSAGSALEFSTRDIGPLSVAPAHAA--AWTRGMLVVEDMPLSDFVAELARYRSGWIN 282 VL AG + GPL A A AW R LV PL++ + EL R+R G I Sbjct: 231 PLVLEAGQRVRLHGGAAGPLEHIDAAALERAWRRHQLVSNARPLTEVLDELNRHRPGRIL 290 Query: 283 CDDA-IGQLKVTGTYPLADTDKVLLMLTRVFP 313 D A + L +T PL DT + L +L P Sbjct: 291 YDQAQLAGLHMTVVLPLDDTQRALQLLVDGVP 322 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,060,939 Number of extensions: 42751 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 6 Length of query: 333 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 247 Effective length of database: 1,212,660 Effective search space: 299527020 Effective search space used: 299527020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)