BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0257.0020.p
         (415 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein...   273   2e-74
gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein...   211   1e-55

>gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 435

 Score =  273 bits (698), Expect = 2e-74,   Method: Compositional matrix adjust.
 Identities = 157/405 (38%), Positives = 231/405 (57%), Gaps = 17/405 (4%)

Query: 6   ARQGALLLHRYVGLVIAGFLTLAGLTGSALAWNDELEAWLAPRLFRVAAPTPGA---VLL 62
           +R   +LLHR+ GL +A FL +  LTGS L ++DEL+AWLAP+L ++A P PG     ++
Sbjct: 18  SRHVLVLLHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQL-QLAQPAPGQSAQAMM 76

Query: 63  DGLQLRRIILSRL-PAADITHTPLRSMPARSVTFRVKGRVSPVTGERAVLDFDEVMINPY 121
           D   LR  +   + P   I+   L + P R+V+F+      P TG+   L +DE+++NPY
Sbjct: 77  DPYLLREKVAQAMGPQVHISQLVLHARPGRTVSFQADPATDPATGKPYRLGYDEILVNPY 136

Query: 122 TGEILGTRKWGRPSWSAESIMPFIYRLHQSMAMG-VVGTYLFGFIALLWTVDCLVGLFLT 180
           TG + G R   + S     +MPF+++LH S+A+    G  L G + LLWT+DC VG+ LT
Sbjct: 137 TGVVQGVRDRDKISLKPADLMPFLFKLHHSLALPRAPGALLLGTVGLLWTLDCFVGMILT 196

Query: 181 LPPPPKRVARKPWLARWTPAWKIRQDARGYKLHFDLHRAAALWFWVLLLLLAWSSVAFNL 240
            P        +P+L +W PAW I+  A  Y+++ DLHRA  LW WV+LLL AWSSV  NL
Sbjct: 197 WP------RARPFLVKWKPAWLIKWRAGFYRVNLDLHRAFGLWCWVMLLLFAWSSVMLNL 250

Query: 241 -SEVYDPVMKKLFGAQSEEALLSRPSLRNMV--PALDWEKARARGRVLMSALADRHNFTI 297
             ++Y PVM ++     + +    P L   +  P   W +A A  R  M   A +    I
Sbjct: 251 RDQIYLPVMSRIL--SFDTSWRPGPPLPQALSGPPQSWPEAHALARRTMDGFARQRGLVI 308

Query: 298 QREDMLAYDSGLGIYRYDVRSSLDLRQRAGSTQVYFDAGTGAQLAVWLPTGGAPGDTIRT 357
             E+ L +D   G+Y Y V S+ DLR   G+T +  DA TG  L  WLP G   G+T   
Sbjct: 309 DFEEKLDFDRRRGVYIYLVHSNADLRADVGNTGLRIDARTGKILGHWLPVGDRDGNTFSN 368

Query: 358 WITSIHMAAIWSWPLKILISLAGIIVAMLSATGVLIWWRRYRARR 402
           W+ ++HM  ++  P +IL+ L G  V++L+ TGV++WW++  ARR
Sbjct: 369 WLGALHMGHVFGLPWRILLVLVGGAVSLLTVTGVVVWWKKRAARR 413


>gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 416

 Score =  211 bits (536), Expect = 1e-55,   Method: Compositional matrix adjust.
 Identities = 145/407 (35%), Positives = 219/407 (53%), Gaps = 27/407 (6%)

Query: 11  LLLHRYVGLVIAGFLTLAGLTGSALAWNDELEAWLAPRLFRV-AAPTPGA-VLLDGLQLR 68
           +LLHR+ GL  A FL +AGLTG+ ++W+ EL+AWL P+LF   +A  PGA   + GL+L 
Sbjct: 6   VLLHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSATQPGAGAPMPGLELA 65

Query: 69  RIILSRLPAADITHTPLRSMPARSVTFRVKGRVSPVTGERAVLDFDEVMINPYTGEILGT 128
           R +    P   +T+   ++ P  ++   V+ RV P TG+   LD++++ ++P+T EI G 
Sbjct: 66  RRVEQAEPRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLDYNQIAVDPFTAEIQGR 125

Query: 129 RKWGRPSWSAESIMPFIYRLHQSMAM----GV-VGTYLFGFIALLWTVDCLVGLFLTLPP 183
           R WG  S S E+++PF+Y+LH +M +    G+  G  L G I ++W +DCL+ L+L+   
Sbjct: 126 RMWGAISLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALYLSF-- 183

Query: 184 PPKRVARKPWLARWTPAWKIRQDARGYKLHFDLHRAAALWFWVLLLLLAWSSVAFNLS-E 242
           P  R  RK    RW           G+KL+FDLHR+  +W W LLL+LA +SV+ NL  +
Sbjct: 184 PNWRSWRKSLAFRWRDG--------GHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQ 235

Query: 243 VYDPVMKKLFGAQSEEALLSRPSLRNMV--PALDWEKARARGRVLMSALADRHNFTIQRE 300
           V  P+++ L            P+    V  P +  E+A A G+    A A +   T +  
Sbjct: 236 VMRPLLQTLSTLTPSPFDTRTPTAPEKVAEPRITREQAVALGQ----AQARKMGLT-EPV 290

Query: 301 DMLAYDSGLGIY--RYDVRSSLDLRQRAGSTQVYFDAGTGAQLAVWLPTGGAPGDTIRTW 358
             + Y    G+Y   Y    +       G+  +YFDA +GA     +P  G+ GD     
Sbjct: 291 GGVFYSGMFGVYGVGYFQPGNDHGDIGLGNAWLYFDANSGAYEGGKIPGRGSAGDIFLQA 350

Query: 359 ITSIHMAAIWSWPLKILISLAGIIVAMLSATGVLIWWRRYRARRETR 405
              +H   I   P +ILIS  G +VAMLS TGV+IW R+ +AR + R
Sbjct: 351 QFPLHSGRILGLPGRILISFMGALVAMLSVTGVIIWVRKRKARLQQR 397


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.326    0.138    0.444 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,272,118
Number of extensions: 52918
Number of successful extensions: 254
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 246
Number of HSP's successfully gapped: 2
Length of query: 415
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 327
Effective length of database: 1,203,190
Effective search space: 393443130
Effective search space used: 393443130
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 132 (55.5 bits)