BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0257.0019.p (290 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 136 2e-33 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 74 1e-14 gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 71 1e-13 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 69 3e-13 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 69 6e-13 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 136 bits (342), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 96/308 (31%), Positives = 157/308 (50%), Gaps = 46/308 (14%) Query: 17 VTRMGYGAMQLSGPHVFGPPKDRQQAIAVLRAALDMGVDHIDTSDFYGPHITNQIIRDAL 76 V+ +G G M +S F +D +++A + AL++G++ +DT+D YGP+ +++ A+ Sbjct: 27 VSAIGLGCMGMSD---FYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEELLGRAI 83 Query: 77 HPYRDDLTIVTKIGARRGADASWQSAM----SPAELESAVYDNLRNLGLEVMDVVNLRLM 132 RD I TK G RR D S A SPA + AV +L+ LG+E +D+ + Sbjct: 84 KGRRDKFFIATKFGIRR--DPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDLY-YQHR 140 Query: 133 FDVAGPAEGDLEESLTALAELKRKGLVRHIGLSNATVKQIAQAKTITDIVCVQNQYNLAH 192 D A P +E ++ LA+L + G +R+IGLS A+ + +A + + +Q++Y+L Sbjct: 141 IDPATP----IEVTMGVLADLVQAGKIRYIGLSEASAATLERAHRVHPVTALQSEYSLWT 196 Query: 193 RDDDA-LVAQLASEGIAYVPFFPLG---------GFSPLQSSTLER-------------- 228 RD +A ++A + GI +V + PLG F L R Sbjct: 197 RDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFRRSNPRFQGENFARNL 256 Query: 229 --------VAAECGATPLQTALAWLLQRSPNILLIPGTSSVAHLQQNIAAAAMKLSPEAV 280 +A + G TP Q ALAW++ + P+I+ IPGT +L+ N + +KL+PE + Sbjct: 257 QLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEDNAGSVGVKLAPEDL 316 Query: 281 AALDGIGP 288 ALD I P Sbjct: 317 QALDAIFP 324 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 74.3 bits (181), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 78/327 (23%), Positives = 142/327 (43%), Gaps = 59/327 (18%) Query: 9 HYRIGDLNVTRMGYGAMQLS----GPHVFGPPKDRQQAIAVLRAALDMGVDHIDTSDFYG 64 HYR R+G +++S G +FG D Q++ ++ +A + GV+ IDT+D Y Sbjct: 2 HYR-------RLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVYT 54 Query: 65 PHITNQIIRDALHPYRDDLTIVTKIGARRGADASWQSAMSPAELESAVYDNLRNLGLEVM 124 + +I+ L R + + +K+G + +S S + + + L L + + Sbjct: 55 KGKSEEIVGKLLAGQRHEWVLASKLGNAMSGKPN-ESNYSRSWIVRETENILTRLDTDYL 113 Query: 125 DVVNLRLMFDVAGPAEGDLEESLTALAELKRKGLVRHIGLSNATVKQIAQAKTITD---- 180 D++ + + + +LEE++ AL +L R G +R G+SN +IA+ + + Sbjct: 114 DILYMHRDYQ-----DENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNV 168 Query: 181 ---IVCVQNQYNLAHRDDDA-LVAQLASEGIAYVPFFPL------GGFSPLQSST----- 225 +VC Q YNL +R + ++ A G+ VP+ P+ G + P Q Sbjct: 169 PLPLVC-QPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDTRA 227 Query: 226 -------------------LERVAAECGATPL---QTALAWLLQRSPNILLIPGTSSVAH 263 +R+ C A + Q A AW+L S +I G ++A Sbjct: 228 GRGDKRMMETEFREESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQ 287 Query: 264 LQQNIAAAAMKLSPEAVAALDGIGPPA 290 ++ AA +K+S E A +D + P Sbjct: 288 MEDYYDAAELKISAEEEALVDSLVTPG 314 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 70.9 bits (172), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 70/263 (26%), Positives = 120/263 (45%), Gaps = 29/263 (11%) Query: 36 PKDRQQAIAVLRAALDMGVDHIDTSDFYGPHITNQIIRDALHPYRDDLTIVTKI----GA 91 P + L+A LD+G+ IDT++ Y +I+ A+ RD + +V+K+ + Sbjct: 29 PAQANAEVRALQAGLDLGMSLIDTAEMYAEGGAERIVAKAIAGRRDQVYLVSKVLPHNAS 88 Query: 92 RRGADASWQSAMSPAELESAVYDNLRNLGLEVMDVVNLRLMFDVAGPAEGDLEESLTALA 151 +RG A+ ++ +L+ LG + +D+ L + A P L ++ + Sbjct: 89 KRGTIAACEA-------------SLKRLGTDRIDLYLLH--WRGAHP----LAATIEGME 129 Query: 152 ELKRKGLVRHIGLSNA---TVKQIAQAKTITDIVCVQNQYNLAHRDDDA-LVAQLASEGI 207 L +G + G+SN + + + +C Q YNL+ R + L+ Q I Sbjct: 130 TLVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGIEYDLLPQAQQAHI 189 Query: 208 AYVPFFPLGGFSPLQSSTLERVAAECGATPLQTALAWLLQRSPNILLIPGTSSVAHLQQN 267 A + + P+ L+ L +A TP Q ALAW+L R P ++ IP + VAH+Q N Sbjct: 190 AVMAYSPIEQGRILKHPALTALAQAHRVTPAQIALAWVL-RQPGVVAIPKAADVAHVQAN 248 Query: 268 IAAAAMKLSPEAVAALD-GIGPP 289 A+ + LS + LD PP Sbjct: 249 RASLDISLSAADLKILDEAFAPP 271 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 69.3 bits (168), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 87/313 (27%), Positives = 136/313 (43%), Gaps = 50/313 (15%) Query: 16 NVTRMGYGAMQLSGPHVFGPPKDRQQAIAVLRAALDMGVDHIDTSDFYGPHITNQIIRDA 75 V+ +G+GA + G +G Q A A L AALD GV IDT+D YG + Q+I Sbjct: 12 QVSEIGFGAWAIGGS--WGDVSA-QDAKAALHAALDAGVSFIDTADVYGDGRSEQLIAQV 68 Query: 76 LHPYRDDLTIVTKIGARRGADASWQSAMSPAELESAVYDNLRNLGLEVMDVVNLRLMFDV 135 L + V RR + A+L + + +L+NLG++ +D+V L Sbjct: 69 LKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLDLVQLH----- 123 Query: 136 AGPAEGDLEESLTA-LAELKRKGLVRHIGLSNATVKQIAQAKTITDIVCVQNQYNL---- 190 P E ++ + L L G +RH G+S V++ +A ++ VQ +N+ Sbjct: 124 CPPTEVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNIFRQR 183 Query: 191 --------AHRDDDALVAQ-------LASEGIAYVPF--------------FPLG-GFS- 219 A R D A++A+ L + A F F +G FS Sbjct: 184 PISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSG 243 Query: 220 -PLQS--STLERVAAEC--GATPLQTALAWLLQRSPNILLIPGTSSVAHLQQNIAAAAM- 273 P + + +E++ A G Q AL W+L S +IPG +VA Q N AA+A+ Sbjct: 244 VPYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAASALP 303 Query: 274 KLSPEAVAALDGI 286 ++ E + AL I Sbjct: 304 PITAEVMQALRAI 316 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 68.6 bits (166), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 75/304 (24%), Positives = 129/304 (42%), Gaps = 66/304 (21%) Query: 33 FGPPKDRQQAIAVLRAALDMGVDHIDTSDFYGP-------HITNQIIRDALHPYRDDLTI 85 FG + + AVLR A D GV H D ++ YGP + + +D L PYRD+L I Sbjct: 37 FGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPPAGSAEENFGRWMAKDFL-PYRDELVI 95 Query: 86 VTKIGARRGADASWQSAMSP--------AELESAVYDNLRNLGLEVMDVVNLRLMFDVAG 137 +K G WQ P L ++ +L+ +GL+ +D+ + Sbjct: 96 SSKAG--------WQMWPGPYGGPSGARKHLIASCEQSLKRMGLDYVDI-----FYSHRV 142 Query: 138 PAEGDLEESLTALAELKRKGLVRHIGLSNATVKQIAQAKTITD-----IVCVQNQYNLAH 192 A+ LEE++ ALA L R+G ++G+S+ + + +A I ++ Q Y++ + Sbjct: 143 DADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEGVPLLIHQPNYSMLN 202 Query: 193 RD-DDALVAQLASEGIAYVPFFP----------LGGFSPLQSST---------------- 225 R + L+ L G+ + F P LGG T Sbjct: 203 RAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVPEGARVTRAPSLPQRMLSEDNLA 262 Query: 226 ----LERVAAECGATPLQTALAWLLQRSPNILLIPGTSSVAHLQQNI-AAAAMKLSPEAV 280 L +A++ G T Q A+AW+L+ + + G +V L ++ A + + E + Sbjct: 263 RIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGARTVEQLDDSLDAVNKLDFTQEEL 322 Query: 281 AALD 284 A++D Sbjct: 323 ASID 326 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 934,798 Number of extensions: 37962 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 153 Number of HSP's successfully gapped: 7 Length of query: 290 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 206 Effective length of database: 1,222,130 Effective search space: 251758780 Effective search space used: 251758780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)