BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0257.0017.p (298 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 129 4e-31 gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 123 1e-29 gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 105 5e-24 gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 91 2e-19 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 129 bits (323), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 93/289 (32%), Positives = 138/289 (47%), Gaps = 8/289 (2%) Query: 12 FIGTGIMGFPMARHLAQAGYRVQAWNRTPEKAQRLSAFGAVTVTEPGDVVRNVDALICML 71 FIG G MG PMA++L +AG+ V + +L G T + V D +I ML Sbjct: 7 FIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKADVVITML 66 Query: 72 SSGPSCDELLLGEHGLIARMRPGSLLVVMSSIPVETARHQAQQAAARGVAYVDAPVSGGE 131 + + L LGE G++A +PG+LL+ S+I E AR A A ARG +DAPVSGG Sbjct: 67 PASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVSGGT 126 Query: 132 KGAMEASLAIMAGGTTQAYEQALPLLEKMGRPT-HVGPAGSGELAKLVNQMMVANNIASV 190 GA +L M GG+ +A+E A P L+KMG+ H G +GSG+ K+ N M++ + Sbjct: 127 NGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLGILMIGT 186 Query: 191 AEALLLAERGGADPAKVREALLGGFADSTILRQH-------ALRMISGDFTPGGPARYQV 243 +EA+ L G DP + E + + L + S + G + Sbjct: 187 SEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAGGFGVDLML 246 Query: 244 KDTSTAIRFAASVGLDLPMLNKSDRLFSDMLKNGDGDLDHSGVIRELRR 292 KD A+ A + +P+ + L+ NG G LD S + L + Sbjct: 247 KDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNMLGK 295 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 123 bits (309), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 79/282 (28%), Positives = 139/282 (49%), Gaps = 2/282 (0%) Query: 9 TVGFIGTGIMGFPMARHLAQAGYRVQAWNRTPEKAQRLSAFGAVTVTEPGDVVRNVDALI 68 VG IG G MG+ +A L +AG+ V A + E + +A G V P ++ VD +I Sbjct: 4 NVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVVI 63 Query: 69 CMLSSGPSCDELLLGEHGLIARMRPGSLLVVMSSIPVETARHQAQQAAARGVAYVDAPVS 128 ++ + + +L G++G ++ M+PGS+++ +++ + A ++ +G+ +DAPVS Sbjct: 64 TLVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDAPVS 123 Query: 129 GGEKGAMEASLAIMAGGTTQAYEQALPLLEKM-GRPTHVGPA-GSGELAKLVNQMMVANN 186 GG A + +M G + Y + +L M G+ +G G G K++NQ++ + Sbjct: 124 GGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVH 183 Query: 187 IASVAEALLLAERGGADPAKVREALLGGFADSTILRQHALRMISGDFTPGGPARYQVKDT 246 IA+ AEA+ L R G +P + E + +S + ++SGD+TP VKD Sbjct: 184 IAASAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFVKDL 243 Query: 247 STAIRFAASVGLDLPMLNKSDRLFSDMLKNGDGDLDHSGVIR 288 + A LP+ + ++F G G D S VI+ Sbjct: 244 GLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIK 285 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 105 bits (261), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 97/287 (33%), Positives = 142/287 (49%), Gaps = 4/287 (1%) Query: 10 VGFIGTGIMGFPMARHLAQAGYRVQAWNRTPEKAQRLSAFGAVTVTEPGDVVRNVDALIC 69 + F+G G MGFPMA +L +AGY V+AWNR+P +RL+A GA P + + D LI Sbjct: 3 IAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADADVLIS 62 Query: 70 MLSSGPSCDELLLGEHGLIARMRPGSLLVVMSSIPVETARHQAQQAAARGVAYVDAPVSG 129 ML+ + E L + G +A +RPG++ V M++I V A A ARGVAYV APV G Sbjct: 63 MLADDDAT-ETSLLDAGALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVLG 121 Query: 130 GEKGAMEASLAIMAGGTTQAYEQALPLLEKMGRPT-HVG-PAGSGELAKLVNQMMVANNI 187 A L I+A G QA PLL+ +G+ T +G AKL M+A+ I Sbjct: 122 RVNVAEAGQLNILAAGEEQALAAVQPLLDVLGQKTWRLGRQPEQANAAKLAMNFMIASAI 181 Query: 188 ASVAEALLLAERGGADPAKVREALLGGFADSTILRQHALRMISGDFTPGG-PARYQVKDT 246 +++EA+ L + G D A E + + + + + F P G +KD Sbjct: 182 GAMSEAVALVQGYGLDKAGFIELATSTAFAAPVYKGYGQAIADDRFEPAGFKLALGLKDV 241 Query: 247 STAIRFAASVGLDLPMLNKSDRLFSDMLKNGDGDLDHSGVIRELRRR 293 A+ + L + + L D L +G+G LD + + R RR Sbjct: 242 RLALEAGEQAHVPLSLASALRDLHIDGLAHGEGHLDWAALSRASARR 288 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 90.5 bits (223), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 73/264 (27%), Positives = 126/264 (47%), Gaps = 2/264 (0%) Query: 10 VGFIGTGIMGFPMARHLAQAGYRVQAWNRTPEKAQRLSAFGAVTVTEPGDVVRNVDALIC 69 +G IG G MG MA L + GY V ++ L+ G V + V+ ++ Sbjct: 4 IGVIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQGLHCVDSIAALAPAVEMVVL 63 Query: 70 MLSSGPSCDELLLGEHGLIARMRPGSLLVVMSSIPVETARHQAQQAAARGVAYVDAPVSG 129 L + ++++ GE GL+ PG ++V ++ + R A + A G+ VDAPVSG Sbjct: 64 SLPTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAPVSG 123 Query: 130 GEKGAMEASLAIMAGGTTQAYEQALPLLEKM-GRPTHVGPAGSGELAKLVNQMMVANNIA 188 G KGA A++ ++ GG+ + + P+L M + HVGP G+G +AKL+N ++ ++ Sbjct: 124 GPKGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVGPVGAGHVAKLLNNLVTGTHLL 183 Query: 189 SVAEALLLAERGGADPAKVREALLGGFADSTILRQHALRMISGD-FTPGGPARYQVKDTS 247 EA +A G D ++ + L G A+S ++ I + F G + KD Sbjct: 184 IAGEAARIAAAAGLDARQIFQGLAAGSANSKVIEVFYPTWIFNEAFDSGFTMKLMRKDLR 243 Query: 248 TAIRFAASVGLDLPMLNKSDRLFS 271 A+ P+ ++ RL++ Sbjct: 244 LAMNLIEETRSTTPLAREAGRLWA 267 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 912,769 Number of extensions: 36767 Number of successful extensions: 176 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 168 Number of HSP's successfully gapped: 4 Length of query: 298 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 213 Effective length of database: 1,217,395 Effective search space: 259305135 Effective search space used: 259305135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)