BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0257.0011.p (271 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310593|ref|YP_003774685.1| phosphate regulon two-component... 415 e-117 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 111 7e-26 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 110 1e-25 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 102 4e-23 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 99 3e-22 >gi|300310593|ref|YP_003774685.1| phosphate regulon two-component sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 436 Score = 415 bits (1066), Expect = e-117, Method: Compositional matrix adjust. Identities = 204/264 (77%), Positives = 228/264 (86%), Gaps = 1/264 (0%) Query: 2 LSVQERKLIVQIIPFENRRQILVTHDATESERIDMMRRDFIANASHELRTPLTVINGFLE 61 +++ ERKLI+QIIPFENRRQILVTHD TESERIDMMRRDF+ANASHELRTPLTVINGFLE Sbjct: 170 MTLHERKLIIQIIPFENRRQILVTHDVTESERIDMMRRDFVANASHELRTPLTVINGFLE 229 Query: 62 IALAQPNLDAEVRTAHLKLMTEQGQRMQNLIGDMLTLTRLESVDYPLRSERVHVRPLLEG 121 IALAQPNL+ R AHLKLMTEQGQRMQNLI DMLTLTRLES+DYPLR+E + VRPLLE Sbjct: 230 IALAQPNLEEGTRMAHLKLMTEQGQRMQNLIDDMLTLTRLESIDYPLRTEVLRVRPLLER 289 Query: 122 ILEEAKALSAGKHQITLDADGPDLQGNVDELRSAFSNLVSNAVRYTPQDGTIKLYWERKP 181 I EE +ALSAGKH+I L DGPDL+GN +ELRSAF+NLV+NAVRYTP+ G I L W+ Sbjct: 290 IAEEGRALSAGKHEIVLTIDGPDLKGNAEELRSAFTNLVTNAVRYTPEGGRISLRWQSD- 348 Query: 182 DGGAQFVVRDNGIGISQEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHRATLG 241 + GA F V+D GIGIS EHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRH ATL Sbjct: 349 EQGAHFAVQDTGIGISPEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLE 408 Query: 242 VESVPDKGSTFTVSFPANVLIEEF 265 + SVPD GSTFTV FP+ I+++ Sbjct: 409 IASVPDHGSTFTVHFPSTQTIQDY 432 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 111 bits (277), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 76/210 (36%), Positives = 115/210 (54%), Gaps = 14/210 (6%) Query: 38 RRDFIANASHELRTPLTVINGFLEIALAQPNLDAEVRTAHLKLMTEQGQRMQNLIGDMLT 97 +R F+A+A+HELRTPLT + L+ LA+ +D E R A + +R +L+ +LT Sbjct: 216 QRAFVADAAHELRTPLTALR--LQTQLAERAVDEEERRAAFADLKNGLERTTHLVHQLLT 273 Query: 98 LTRLESVDYPLRSERVHVRPLLEGILEEAKALSA-GKHQITLDADGPD-----LQGNVDE 151 L R E + +V +R LL ++ + +++ G + L G D + GN D Sbjct: 274 LARQEP--DAMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNADA 331 Query: 152 LRSAFSNLVSNAVRYTPQDGTIKLYWERKPDGGAQFVVRDNGIGISQEHISRLTERFYRV 211 LR F+NLV NA+RYTP G + + + G VV+D+G GI++ + R+ +RFYR Sbjct: 332 LRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDRFYRA 391 Query: 212 DKSR----SRETQGTGLGLAIVRHVLLRHR 237 S+ SR G+GLGLAIVR + HR Sbjct: 392 QHSQEGSGSRAAFGSGLGLAIVRQIADLHR 421 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 110 bits (276), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 74/232 (31%), Positives = 123/232 (53%), Gaps = 8/232 (3%) Query: 32 ERIDMMRRDFIANASHELRTPLTVINGFLEIALAQPNLDAEVRTAHLKLMTEQGQRMQNL 91 ER + +RR+F+A+ SHELRTPL ++NG LE AL L + +T L+ + + + + L Sbjct: 242 ERNEKLRREFMADVSHELRTPLAILNGQLE-ALEDGVLQPDAQT--LRSLKSEVEHLNKL 298 Query: 92 IGDMLTLTRLESVDYPLRSERVHVRPLLE---GILEEAKALSAGKHQITLDADGPDLQGN 148 +GD+ L+ ++ R + + PLL + + + + L A P + G+ Sbjct: 299 VGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCELPASMP-VHGD 357 Query: 149 VDELRSAFSNLVSNAVRYTPQDGTIKLYWERKPDGGAQFVVRDNGIGISQEHISRLTERF 208 L+ SNL N++RYT G ++++ + V D+ G+ E ++RL ERF Sbjct: 358 AARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHW-HLVFDDSEPGVPPEVMARLFERF 416 Query: 209 YRVDKSRSRETQGTGLGLAIVRHVLLRHRATLGVESVPDKGSTFTVSFPANV 260 +RV+ SR+R + G GLGLAI R ++ H+ T+G + P G V PA + Sbjct: 417 FRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPARL 468 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 102 bits (254), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 84/241 (34%), Positives = 127/241 (52%), Gaps = 12/241 (4%) Query: 26 HDATES--ERIDMMRRDF------IANASHELRTPLTVINGFLEIALAQPNLDAEVRTAH 77 H+ ES E +D + R F A+ +H++RTPL + ++AL +P E + A Sbjct: 225 HEIVESFNEMLDRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQ-AL 283 Query: 78 LKLMTEQGQRMQNLIGDMLTLTRLESVDYPLRSERVHVRPLLEGILEEAKALSAGKHQIT 137 L E+ +R+ ++ ML L R E+ L+ + + V L I + + + A + Q+ Sbjct: 284 LSSNIEEYERLSRMMESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGI-ADEAQVR 342 Query: 138 LDADGPD-LQGNVDELRSAFSNLVSNAVRYTPQDGTIKLYWERKPDGGAQFVVRDNGIGI 196 L G LQ + LR A NLV+NA+RYTP DG I+L + DG + V + G GI Sbjct: 343 LVVQGAGRLQADATLLRRAVGNLVANAIRYTPADGVIRLQVDAAGDG-MEISVSNPGAGI 401 Query: 197 SQEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHRATLGVESVPDKGSTFTVSF 256 E + RL +RFYR D SRS +GLGLAIVR +++ H V S P + + F++ F Sbjct: 402 PAEQLERLFDRFYRADPSRSSAGGSSGLGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRF 461 Query: 257 P 257 P Sbjct: 462 P 462 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 99.4 bits (246), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 80/250 (32%), Positives = 135/250 (54%), Gaps = 18/250 (7%) Query: 19 RRQILVTHDATESERIDMMRRDFIANASHELRTPL--TVINGFLEIALAQPNL-DAEVRT 75 + Q+++ ATE ER + + +F+AN SHELRTPL T+I L Q NL D +VR Sbjct: 456 KTQVVLVEKATELERANQYKSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQVRF 515 Query: 76 AHLKLMTEQGQRMQNLIGDMLTLTRLESVDYPLRSERVHVRPLLEGILEEAKALSAGKH- 134 A + ++ G + LI D+L L+++E+ + E + L + L + A++A + Sbjct: 516 A--QTISSAGNDLLALINDILDLSKMEAGKLDIVPESFPLTKLADE-LSASFAITAQQKG 572 Query: 135 ---QITLDADGPD-LQGNVDELRSAFSNLVSNAVRYTPQ-DGTIKLYWERKPDGGAQ--F 187 ++ ++ P ++ ++ L NL+SNA+++T + + ++++Y PD G + F Sbjct: 573 LGFKVEVEPGVPQRMETDIQRLGQILKNLLSNAIKFTEKGEVSLRIY----PDAGGRIAF 628 Query: 188 VVRDNGIGISQEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHRATLGVESVPD 247 VRD GIGI ++ + E F + D S R+ GTGLGL+I R + + V S Sbjct: 629 AVRDTGIGIGEDQHQFIFEAFRQADGSTHRKYGGTGLGLSISRDLAHLLGGDIRVTSRLG 688 Query: 248 KGSTFTVSFP 257 +GS FT+S P Sbjct: 689 EGSIFTLSLP 698 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 824,760 Number of extensions: 34644 Number of successful extensions: 241 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 39 Length of query: 271 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 187 Effective length of database: 1,222,130 Effective search space: 228538310 Effective search space used: 228538310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)