BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0257.0005.p
         (311 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310589|ref|YP_003774681.1| phosphate ABC transporter perme...   489   e-140
gi|300310588|ref|YP_003774680.1| phosphate ABC transporter perme...    59   5e-10

>gi|300310589|ref|YP_003774681.1| phosphate ABC transporter permease
           [Herbaspirillum seropedicae SmR1]
          Length = 298

 Score =  489 bits (1260), Expect = e-140,   Method: Compositional matrix adjust.
 Identities = 250/311 (80%), Positives = 272/311 (87%), Gaps = 13/311 (4%)

Query: 1   MNQQVTPQAGAKDVAVPLNSPLNPVYRRRLLQHRVGMLFSFLAMAAGLAVLAWILLTLVI 60
           M+Q V       D A      +NPVYR+RL+QHR+GMLFSFLAM  GL VLAWILLTLV 
Sbjct: 1   MSQTVQAGVQHADQA----QAMNPVYRKRLMQHRMGMLFSFLAMGLGLFVLAWILLTLVF 56

Query: 61  KGFGALNFGMLTQNTPSPGGDGGGLLNPIIGSLLMVGLGTVISTPIGILAGIYLAEYGEE 120
           KG  ALN G+LT+NTP PG  GGGLLNPI+GSL+MVG+ T+ISTPIGILAG+YLAEYGEE
Sbjct: 57  KGVAALNLGLLTENTPGPGDAGGGLLNPIVGSLMMVGISTLISTPIGILAGVYLAEYGEE 116

Query: 121 SKLAQVTRFVTDIMLSAPSIVIGLFVYAIYVANVGHFSGWAGTFAISLIAIPVVVRTTDN 180
           SKLAQVTRFVTDIMLSAPSIVIGLF+YAIYVANVGHFSGWAGTFAISLIAIPVVVRTTDN
Sbjct: 117 SKLAQVTRFVTDIMLSAPSIVIGLFIYAIYVANVGHFSGWAGTFAISLIAIPVVVRTTDN 176

Query: 181 MLKLVPSSLREAAFSLESSDDGAPAXAPRWKVAMMVRLRAVKAGVVTGVLLAVARISGET 240
           MLKLVPS LREAAF+L          APRWKVA++VRLRAVKAGVVTGVLLAVARISGET
Sbjct: 177 MLKLVPSGLREAAFAL---------GAPRWKVAIIVRLRAVKAGVVTGVLLAVARISGET 227

Query: 241 APLLFTALNNQFFSTNMNQPIANLPVVINQFAMSPYDNWVALAWGGALLITFSVLALNIL 300
           APLLFTALNNQFFSTNMN+PIANLPVVINQFAMSPYDNWV+LAWGGALLITFSVL LN+L
Sbjct: 228 APLLFTALNNQFFSTNMNRPIANLPVVINQFAMSPYDNWVSLAWGGALLITFSVLLLNVL 287

Query: 301 SRTMFSQRVPG 311
           SRT+F Q++PG
Sbjct: 288 SRTLFKQQIPG 298


>gi|300310588|ref|YP_003774680.1| phosphate ABC transporter permease
           [Herbaspirillum seropedicae SmR1]
          Length = 313

 Score = 58.9 bits (141), Expect = 5e-10,   Method: Compositional matrix adjust.
 Identities = 67/263 (25%), Positives = 117/263 (44%), Gaps = 47/263 (17%)

Query: 25  VYRRRLLQ----HRVGMLFSFLAMAAGLAVLAWILLTLVIKG---FGALNFGMLTQNTPS 77
           V RR+ LQ    H++ ++F+     + L +L  I+++L+I     F A   G +T+    
Sbjct: 3   VMRRQRLQDFFFHKITLIFAL----SVLLMLGGIIVSLIIGAAPAFQAFGPGFITRVEWD 58

Query: 78  PGGDGGGLLNPIIGSLLMVGLGTVISTPIGILAGIYLAEYGEESKLAQVTRFVTDIMLSA 137
           P  +  G +  I+G+L    +  +I+ P+     ++L E    +KL +      +++   
Sbjct: 59  PTNNEFGAMIAIVGTLSTSIIALLIAFPVSFGIALFLTEICP-AKLRRPLGTCVELLAGV 117

Query: 138 PSIVIG---LFVYAIYVANVGH----------------FSG-------WAGTFAISLIAI 171
           PSI+ G   LFV+A   +  G                 FSG             +S++ I
Sbjct: 118 PSIIYGMWGLFVFAPLFSEYGQPFLKATLGQIPGIGVLFSGPTMGIGILTAGLILSVMII 177

Query: 172 PVVVRTTDNMLKLVPSSLREAAFSLESSDDGAPAXAPRWKVAMMVRLRAVKAGVVTGVLL 231
           P +     ++ ++VP+ L+E+A+ L  +         RW+V   V L   K GVV GV+L
Sbjct: 178 PFISSVMRDVFEVVPAVLKESAYGLGCT---------RWEVVRKVVLPYTKTGVVGGVML 228

Query: 232 AVARISGETAPLLFTALNNQFFS 254
            + R  GET  + F   N+   S
Sbjct: 229 GLGRALGETMAVTFVIGNSHRLS 251


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.138    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 951,888
Number of extensions: 40301
Number of successful extensions: 174
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 175
Number of HSP's successfully gapped: 2
Length of query: 311
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 226
Effective length of database: 1,217,395
Effective search space: 275131270
Effective search space used: 275131270
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 130 (54.7 bits)