BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0257.0004.p
         (351 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310588|ref|YP_003774680.1| phosphate ABC transporter perme...   563   e-162
gi|300310589|ref|YP_003774681.1| phosphate ABC transporter perme...    61   2e-10

>gi|300310588|ref|YP_003774680.1| phosphate ABC transporter permease
           [Herbaspirillum seropedicae SmR1]
          Length = 313

 Score =  563 bits (1451), Expect = e-162,   Method: Compositional matrix adjust.
 Identities = 280/313 (89%), Positives = 298/313 (95%)

Query: 39  LATMRKQRIQDFLFHKVTLIFALSVLVMLGGIIVSLFIGAWPAFHEFGPGFITRVEWDPV 98
           +A MR+QR+QDF FHK+TLIFALSVL+MLGGIIVSL IGA PAF  FGPGFITRVEWDP 
Sbjct: 1   MAVMRRQRLQDFFFHKITLIFALSVLLMLGGIIVSLIIGAAPAFQAFGPGFITRVEWDPT 60

Query: 99  NDQYGAAIAIVGTLTTSLIALLIAFPVSFGIALFLTEICPSRLRRPLGTAVELLAGVPSI 158
           N+++GA IAIVGTL+TS+IALLIAFPVSFGIALFLTEICP++LRRPLGT VELLAGVPSI
Sbjct: 61  NNEFGAMIAIVGTLSTSIIALLIAFPVSFGIALFLTEICPAKLRRPLGTCVELLAGVPSI 120

Query: 159 IYGMWGLFVFAPLFAEYVQPALKATLGQLPVIGALFQGPTMGIGILTAGLILSVMIIPFI 218
           IYGMWGLFVFAPLF+EY QP LKATLGQ+P IG LF GPTMGIGILTAGLILSVMIIPFI
Sbjct: 121 IYGMWGLFVFAPLFSEYGQPFLKATLGQIPGIGVLFSGPTMGIGILTAGLILSVMIIPFI 180

Query: 219 ASVMRDVFEVVPAVLKESAYGLGCTRWEVVRKVVLPYTKTGVVGGVMLGLGRALGETMAV 278
           +SVMRDVFEVVPAVLKESAYGLGCTRWEVVRKVVLPYTKTGVVGGVMLGLGRALGETMAV
Sbjct: 181 SSVMRDVFEVVPAVLKESAYGLGCTRWEVVRKVVLPYTKTGVVGGVMLGLGRALGETMAV 240

Query: 279 TFVIGNSHRLSWSLFAAGNSIASTLANEIAEASSKLHVSSLFALGLILFVITFIVLAVAK 338
           TFVIGNSHRLSWSLF+AGNSIASTLANEIAEASSKLHVSSLFALGLILFVITFIVL+ AK
Sbjct: 241 TFVIGNSHRLSWSLFSAGNSIASTLANEIAEASSKLHVSSLFALGLILFVITFIVLSAAK 300

Query: 339 VMLMGMTRKEGAK 351
           +MLMGMTRKEGAK
Sbjct: 301 IMLMGMTRKEGAK 313


>gi|300310589|ref|YP_003774681.1| phosphate ABC transporter permease
           [Herbaspirillum seropedicae SmR1]
          Length = 298

 Score = 60.8 bits (146), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 72/273 (26%), Positives = 118/273 (43%), Gaps = 45/273 (16%)

Query: 25  DSAMQQAQHAHAALLATMRKQRIQDFLFHKVTLIFALSVLVMLGGIIVSLFIGAW----- 79
            + +Q A  A A +    RK+ +Q    H++ ++F  S L M  G    LF+ AW     
Sbjct: 6   QAGVQHADQAQA-MNPVYRKRLMQ----HRMGMLF--SFLAMGLG----LFVLAWILLTL 54

Query: 80  --PAFHEFGPGFITRVEWDPVNDQYGAAIAIVGTLTTSLIALLIAFPVSFGIALFLTEIC 137
                     G +T     P +   G    IVG+L    I+ LI+ P+     ++L E  
Sbjct: 55  VFKGVAALNLGLLTENTPGPGDAGGGLLNPIVGSLMMVGISTLISTPIGILAGVYLAEYG 114

Query: 138 P-SRLRRPLGTAVELLAGVPSIIYGMWGLFVFAPLFAEYVQPALKATLGQLPVIGALFQG 196
             S+L +      +++   PSI+ G++       ++A YV     A +G        F  
Sbjct: 115 EESKLAQVTRFVTDIMLSAPSIVIGLF-------IYAIYV-----ANVGHFSGWAGTFA- 161

Query: 197 PTMGIGILTAGLILSVMIIPFIASVMRDVFEVVPAVLKESAYGLGCTRWEVVRKVVLPYT 256
                        +S++ IP +     ++ ++VP+ L+E+A+ LG  RW+V   V L   
Sbjct: 162 -------------ISLIAIPVVVRTTDNMLKLVPSGLREAAFALGAPRWKVAIIVRLRAV 208

Query: 257 KTGVVGGVMLGLGRALGETMAVTFVIGNSHRLS 289
           K GVV GV+L + R  GET  + F   N+   S
Sbjct: 209 KAGVVTGVLLAVARISGETAPLLFTALNNQFFS 241


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.326    0.140    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 888,799
Number of extensions: 32746
Number of successful extensions: 196
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 196
Number of HSP's successfully gapped: 3
Length of query: 351
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 265
Effective length of database: 1,212,660
Effective search space: 321354900
Effective search space used: 321354900
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 131 (55.1 bits)