BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0004.p (219 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312545|ref|YP_003776637.1| iron-regulated membrane protein... 64 9e-12 gi|300311427|ref|YP_003775519.1| signal transduction histidine k... 59 3e-10 >gi|300312545|ref|YP_003776637.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 63.9 bits (154), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 42/113 (37%), Positives = 61/113 (53%), Gaps = 15/113 (13%) Query: 25 DQNARWTVVIAVLAVLIALALGLSIVQWVVRDVRRLSRARARGPDRLRPLFPKLPAGQTG 84 D RW + L+ + +A G + W V+ ++ ++A A+G RP G Sbjct: 350 DPWLRWLFFLCGLSGYLMVASGALL--WAVKTRQKEAKAIAQGA---RP----------G 394 Query: 85 FGLRLVERGNTACIAGLLAALPAFFLANRLLPLDMAQRSEWEIHCFFLCWGAA 137 +GLRLV+ N IAGL A ++F ANRLLPL+M QR + EI+ FF+ W A Sbjct: 395 WGLRLVDALNIGAIAGLPIAFASYFWANRLLPLEMTQRPQAEINVFFIAWAVA 447 >gi|300311427|ref|YP_003775519.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 560 Score = 58.9 bits (141), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 24/61 (39%), Positives = 42/61 (68%) Query: 3 DGAGLSWVTVVAVPRSDFLDTLDQNARWTVVIAVLAVLIALALGLSIVQWVVRDVRRLSR 62 D AGL W+ + A PRSDFL ++ T+++ ++AV + +G +++WV+RD+RRL++ Sbjct: 304 DAAGLDWLAISAAPRSDFLGSVTSGVYQTLMLGLMAVCLTFVIGFLLLRWVLRDIRRLTQ 363 Query: 63 A 63 A Sbjct: 364 A 364 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.137 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 530,142 Number of extensions: 14663 Number of successful extensions: 68 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 68 Number of HSP's successfully gapped: 2 Length of query: 219 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 137 Effective length of database: 1,231,600 Effective search space: 168729200 Effective search space used: 168729200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)