BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0256.0003.p
         (501 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312545|ref|YP_003776637.1| iron-regulated membrane protein...   322   6e-89

>gi|300312545|ref|YP_003776637.1| iron-regulated membrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 546

 Score =  322 bits (824), Expect = 6e-89,   Method: Compositional matrix adjust.
 Identities = 211/507 (41%), Positives = 276/507 (54%), Gaps = 26/507 (5%)

Query: 1   MTGLHLWAGVLTGWLLYAIFITGGVSYFRDELTQWLRPEIPA---RPATLDAAQVGQRAL 57
           M  LH W+G+L  WLL  +F  G  SYF+DE++ W+ PE+ A   +P     A   +RA+
Sbjct: 9   MAWLHTWSGLLVCWLLLLVFAGGSASYFKDEISFWMMPELHATQRQPVPQPLAV--ERAI 66

Query: 58  DALSIMAADSTLWQINLPGKRGNAIEVAW----------QTAESEHRAWL-DPLSGKPVV 106
             L   A  +  W I+LP  R  A+ V W           +A S   + L D  +G+ + 
Sbjct: 67  AHLQQQAPQAERWFISLPSARSVAMRVGWIAPPPKDGPAGSARSRFTSELVDVTTGQKMA 126

Query: 107 A-RATEAGEFFYYVHFSLHYLPRILARWLVGLCAMFSLVVLVSGVIVHRRIFSDFFTFRP 165
           A R T  GEF Y +HF LHY+P   ARW+V + A+F LV +VSGVI H+RIF DFFTFR 
Sbjct: 127 AVRETRGGEFLYRLHFDLHYMPVQWARWIVSIGALFMLVAIVSGVITHKRIFKDFFTFRA 186

Query: 166 GKGARSWLDAHNSFSVLGLPFHVMIVYTGLVTLMFTYMPWGALAALGPEQARPALARVMQ 225
            KG RSWLDAHN+ +VL LPFH+MI YTGLVTLMF  MPWG   A   +    A    + 
Sbjct: 187 NKGQRSWLDAHNAAAVLALPFHLMITYTGLVTLMFMIMPWGVQTAYPGKGGESAFFEEVF 246

Query: 226 AQVDVPAR-TGVAAPLAALPPLMEQATARWGKDSATRIIVGNPGDAVARIAIVRGDSGRL 284
             V    +  G AAPL A+ PL+ QA   W       + + +PGDA A + + R  +  L
Sbjct: 247 PNVAGRGKAAGQAAPLTAIGPLVTQAQQHWQGAPLALVTINHPGDAKATVVLTRQKAATL 306

Query: 285 SVAPASLLFHGVSG-SLLETRDSPGPVLKTWGALYALHLGRFADHALRWLYFLSSLTGAA 343
           S    +L+F GVSG       + P     T   LY LHLG FAD  LRWL+FL  L+G  
Sbjct: 307 SYLQPALVFDGVSGVQKASVGEQPSNTGVTRAVLYGLHLGHFADPWLRWLFFLCGLSGYL 366

Query: 344 MVATGMILWTVKRRNK-----LPAGQTGFGLRLVERGNTACIAGLLAALPAFFLANRLLP 398
           MVA+G +LW VK R K         + G+GLRLV+  N   IAGL  A  ++F ANRLLP
Sbjct: 367 MVASGALLWAVKTRQKEAKAIAQGARPGWGLRLVDALNIGAIAGLPIAFASYFWANRLLP 426

Query: 399 LDMAQRSEWEIHCFFLCWGAALLHACWRRSAQAWREQLLAAALLWATVPLASGWNSGRDL 458
           L+M QR + EI+ FF+ W  A + A  R     WR QL A ALL+A + + + + +   L
Sbjct: 427 LEMTQRPQAEINVFFIAWAVAAVLAQLRPGLAMWRVQLGAGALLFAGIVVLNIFTTHSHL 486

Query: 459 LQS--LQSSDWLFVTFDAMFLTVAAIL 483
             +  L    W    FD   L + A L
Sbjct: 487 GVTLLLGRGPWPVAGFDLTMLALGASL 513


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.326    0.138    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,629,855
Number of extensions: 68232
Number of successful extensions: 353
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 348
Number of HSP's successfully gapped: 1
Length of query: 501
Length of database: 1,619,870
Length adjustment: 89
Effective length of query: 412
Effective length of database: 1,198,455
Effective search space: 493763460
Effective search space used: 493763460
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 133 (55.8 bits)