BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0001.p (377 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313794|ref|YP_003777886.1| ferrichrome-iron receptor prote... 202 4e-53 gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein... 197 9e-52 gi|300311652|ref|YP_003775744.1| TonB-dependent siderophore rece... 151 7e-38 gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisideropho... 145 7e-36 gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore rece... 117 1e-27 >gi|300313794|ref|YP_003777886.1| ferrichrome-iron receptor protein [Herbaspirillum seropedicae SmR1] Length = 817 Score = 202 bits (514), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 145/385 (37%), Positives = 208/385 (54%), Gaps = 27/385 (7%) Query: 1 VSVITADELADRKVETLDEALRYTAGVTPNMKPWAVD---EFSMLRGFDLGTAGIFRDGL 57 +SV+TA+ +A T +AL YT GV N+ P+ D ++ LRGFD + G + DGL Sbjct: 170 ISVVTAERIAAMGATTARDALGYTPGV--NISPYGSDSRYDWINLRGFDAYSPGFYLDGL 227 Query: 58 -LTSGRSYAA-PIEPYGLERLEVLRGPASVMYGQSPPGGMINAVSKRPSATAVREIGVEY 115 L + S+A E YG+E+L VLRGPASV+YGQ+ PGG++N VSK PSA A RE+ V+ Sbjct: 228 PLRNANSFAVWRTENYGIEQLAVLRGPASVLYGQAGPGGVVNVVSKLPSADAPRELQVQL 287 Query: 116 GTYDRKQLKADVGGALDTGGEWTWRVTMLARESGTRLAFDRDDRVYVAPALTWQPNAATR 175 G+ R+Q+ AD+GGA+D G +RV + R++ ++DR+Y+AP+L W+ +AAT Sbjct: 288 GSDRRRQVGADIGGAIDADGRLLYRVIGVVRDAELPAGGMKNDRLYLAPSLVWKISAATS 347 Query: 176 LTLLAQYQKDDQS-YAWPNQLQN---PGALGQPDPRVNLGGKD--NRWKRENHTFGYEFE 229 LTL A +D Y L P A+G P G + + ++ FGY Sbjct: 348 LTLQAALLRDQGGVYTRTRPLVGSLVPTAIGTTIPSELYTGNPHFDHFSQDQELFGYRLA 407 Query: 230 HRFNDTWTLQQNLRYTELERDETNV-FPRVLLADG---------RTVTR-LFYPRSSAWR 278 HRF++ + Q+ R + D V PR + + +TR LF R SA Sbjct: 408 HRFDERFAFTQSARVGHMSTDYRAVQTPRFATLNAANPNDPVNFQNLTRNLFGSRESATM 467 Query: 279 GLQVDTRMQADFRTGELGHLVLLGLDYADSRTTDRFPNSITAMPGFDLYNPVYGNNATIV 338 +D ++QADF TG + H VL G+DY SR D S + P ++ P Y N +V Sbjct: 468 -FSIDNQLQADFSTGAVRHTVLAGVDYQRSR-FDAVSFSGGSAPLLNIAAPSYPNGPFVV 525 Query: 339 RSATPRIEQYPLRQTGLYLQDQVKW 363 A + L QTG+YLQDQ+KW Sbjct: 526 -PAPYSDNRAVLTQTGIYLQDQIKW 549 >gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 197 bits (502), Expect = 9e-52, Method: Compositional matrix adjust. Identities = 138/382 (36%), Positives = 199/382 (52%), Gaps = 32/382 (8%) Query: 1 VSVITADELADRKVETLDEALRYTAGVTPNMKPWAVD----EFSMLRGF-DLGTAGIFRD 55 VSV+T DE+ R V +L + LRY G N + VD E+ LRGF + F + Sbjct: 72 VSVVTRDEMDARGVTSLLDVLRYMPGA--NSETHGVDPRGYEYFNLRGFINAQVTSNFLN 129 Query: 56 GLLTSGRSYAA-PIEPYGLERLEVLRGPASVMYGQSPPGGMINAVSKRPSATAVREIGVE 114 GL + E YG+ER+EVLRGP SV +GQ+ PGG+IN VSK P E+GV+ Sbjct: 130 GLRQIPGGFGQFRTETYGMERIEVLRGPGSVTFGQADPGGVINRVSKLPGMDIPNELGVD 189 Query: 115 YGTYDRKQLKADVGGALDTGGEWTWRVTMLARESGTRLAFDR-----DDRVYVAPALTWQ 169 G+++RKQ+ AD+ G GE+ WRV L +S + F+ ++R Y+AP+LTW+ Sbjct: 190 IGSFNRKQVVADLNGHTQ-DGEYLWRVVGLGLDSNNQYKFNNGQSGDNNRFYLAPSLTWR 248 Query: 170 PNAATRLTLLAQYQKD-DQSYAW----PNQLQNPGALGQPDPRVNLGGKDNRWKRENHTF 224 P+A+T LTL+A Y D S W P + +G P+ +R + +F Sbjct: 249 PSASTSLTLMADYMNDRSGSSRWTAVRPGNVLTHTLIGDPN--------FDRQSNDQWSF 300 Query: 225 GYEFEHRFNDTWTLQQNLRYTELERDETNVFPRVLLADGRTVTRLFYPRSSAWRGLQVDT 284 GY+FEH+F++ WT +QN R L+ + P L G +TR +S VD Sbjct: 301 GYQFEHKFDNAWTFRQNFRQANLKSVYAALNPGTLT--GSVLTRGTTVYNSQVDNTLVDN 358 Query: 285 RMQADFRTGELGHLVLLGLDYADSRTTDRFPNSITAMPGFDLYNPVYGNNATIVRSATPR 344 +++ + G H VL GLD+ SR DR A P ++ NPVYG + Sbjct: 359 QLEGRLKWGATEHTVLFGLDW--SRMYDREVRYRGAAPSLNIDNPVYGVTIPAATTLFGN 416 Query: 345 IEQYPLRQTGLYLQDQVKWXAR 366 +E+ + QTGLY QDQ+K+ R Sbjct: 417 LEE-NMNQTGLYAQDQIKFDQR 437 >gi|300311652|ref|YP_003775744.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 832 Score = 151 bits (382), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 124/380 (32%), Positives = 186/380 (48%), Gaps = 33/380 (8%) Query: 1 VSVITADELADRKVETLDEALRYTAGV---TPNMKPWAVDEFSMLRGF-DLGTAGIFRDG 56 VSV+T E+ R ++ + LRY G T + P D F+ LRGF + T + +G Sbjct: 199 VSVVTRAEMEARGANSILDVLRYMPGANTETHGVDPRGYDYFN-LRGFINAQTTSNYLNG 257 Query: 57 LLTSGRSYAA-PIEPYGLERLEVLRGPASVMYGQSPPGGMINAVSKRPSATAVREIGVEY 115 L + E YGLER+EVLRG S +GQ+ PGG++N VSK + A E+ V+ Sbjct: 258 LRQIPSGFGMFRTETYGLERVEVLRGANSASFGQADPGGVVNRVSKLAGSGARNELMVDV 317 Query: 116 GTYDRKQLKADVGGALDTGGEWTWRVTMLARESGTRLAFDR-----DDRVYVAPALTWQP 170 G++ R+Q+ AD+ G LD G R+ L + T+ + +DR+Y+AP+L +P Sbjct: 318 GSFQRRQVAADLSGDLDPEGRVQARLVGLLLDGDTQFRYANGRAGDNDRLYLAPSLKIRP 377 Query: 171 NAATRLTLLAQYQKDDQSYAWPNQLQNPGA-----LGQPDPRVNLGGKDNRWKRENHTFG 225 +A T L LLA+Y KD ++ G+ LG PD ++ + E + G Sbjct: 378 SADTSLILLAEYLKDRSGSGRWTAVRADGSQTHTLLGDPD--------FDQQRGEQWSLG 429 Query: 226 YEFEHRFNDTWTLQQNLRYTELERDETNVFPRVLLADGRTVTRLFYPRSSAWRGLQVDTR 285 + EHR + TWTL+Q+ R L V P +G ++R + +D + Sbjct: 430 WMLEHRLDATWTLRQHFRQAALRSQYAAVNPGSF--NGSILSRSTAVYDTQVENTLLDNQ 487 Query: 286 MQADFRTGELGHLVLLGLDYADSRTTDRFPNSITAMPGFDLYNPVYGNNATIVRSATPRI 345 A + G+ H VLLGLD+ S + R P D+ NPVY + +AT R Sbjct: 488 ALARVQWGQAEHQVLLGLDW--SHMSAREQRYRGVAPSLDIDNPVY---TQPIPAATTRF 542 Query: 346 EQY--PLRQTGLYLQDQVKW 363 L QTGLY QDQ+++ Sbjct: 543 GDLDQTLVQTGLYAQDQIRY 562 >gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisiderophore receptor signal peptide protein, tonB-dependent receptor [Herbaspirillum seropedicae SmR1] Length = 715 Score = 145 bits (365), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 115/383 (30%), Positives = 183/383 (47%), Gaps = 32/383 (8%) Query: 1 VSVITADELADRKVETLDEALRYTAGVTPNM---KPWAVDEFSMLRGFDLGTAGIFRDGL 57 +SV+T + ++ +L +AL+ +GV + W D+ ++RG + + ++ DGL Sbjct: 84 ISVVTRAMMDSQQAVSLSDALKNVSGVVAQQYGRRGW--DDL-IIRG-QVASDSLYLDGL 139 Query: 58 LTSGRSYAAPIEPYGLERLEVLRGPASVMYGQSPPGGMINAVSKRPSATAVREIGVEYGT 117 T+ S A + YGL+++EVL+GP S++YG PGG++N VSKRP + + V G+ Sbjct: 140 RTTASSRVAE-QLYGLQQVEVLKGPGSLLYGLVLPGGLVNMVSKRPQGSDFANVDVTIGS 198 Query: 118 YDRKQLKADVGGALDTGGEWTWRVTMLARESGTRLAFDRDDRV-----YVAPALTWQPNA 172 +D Q D+ +L G+ +R+ L S D D V Y+AP+L+ A Sbjct: 199 HDLYQSTVDLNKSLSENGKQAFRINGLISNSD-----DATDHVWFKNRYIAPSLSLDLGA 253 Query: 173 ATRLTLLAQYQKDDQSYAWPNQLQ-----NPGALGQPDPRVNLGGKDNRWKRENHT-FGY 226 T T+L YQ +++Y L P A G + +G R N T GY Sbjct: 254 RTDFTILTSYQ--ERNYVRQQGLPLSGSITPNASGPLARSLFIGEPGARPYAANETRIGY 311 Query: 227 EFEHRFNDTWTLQQNLRYTELERDETNVFPRVLLADGRTVTRLFYPRSSAWRGLQVDTRM 286 HRF+D WTL NLRY + + L G ++TR ++ + +DT + Sbjct: 312 SLTHRFDDGWTLNNNLRYQQFTMSGQLISINNL--TGTSLTRSAMDQTFEGQTFTMDTNV 369 Query: 287 QADFRTGELGHLVLLGLDYADSRTTDRFPNSITAMPGFDLYNPVYGNNA---TIVRSATP 343 Q F T H V LG DY +SR +R + + P ++YNP YG T + S Sbjct: 370 QRSFATAWGKHEVTLGTDYMNSRELERTL-TCSVTPLSNIYNPSYGARVVCPTQLTSTNA 428 Query: 344 RIEQYPLRQTGLYLQDQVKWXAR 366 ++ +R TG Y +D++ R Sbjct: 429 KLNDTSVRDTGFYARDKISLGER 451 >gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 117 bits (293), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 109/377 (28%), Positives = 158/377 (41%), Gaps = 21/377 (5%) Query: 1 VSVITADELADRKVETLDEALRYTAGVTPNMKPWAVDEFSMLRGF---DLGTAGIFRDGL 57 V V++ + D LD+ L Y +GV + + +RGF + G I +G Sbjct: 72 VQVVSRQLIDDLAATRLDDVLLYVSGVARQNNFGGLWDNYSIRGFSGSENGGMNILWNGF 131 Query: 58 LTSGRSYAAPIEPYGLERLEVLRGPASVMYGQSPPGGMINAVSKRPSATAVREIGVEYGT 117 S R YA P + +E ++ L+GPA+ +YG S PGG IN V+K+P + + + G+ Sbjct: 132 -ASNRGYAPPRDMANVESVDFLKGPAAALYGNSEPGGTINVVTKKPQFKSANALDLSIGS 190 Query: 118 YDRKQLKADVGGALDTGGEWTWRVTMLARESGTRLAFDRDDRVYVAPALTWQPNAATRLT 177 D + D GAL + +R+ G+ R +R + APA TW T L Sbjct: 191 NDAYRSAVDSTGALSS--TVAYRLNAAMEHKGSMRDQVRSEREFFAPAFTWLLTENTILN 248 Query: 178 ----LLAQYQKDDQSYAWPNQLQNPGALGQPDPRVNLGGKDNRWKRENHTFGYEFEHRFN 233 L Q D+ N N G L P R D K +N E R N Sbjct: 249 YEGEFLRQKAPLDRGIVAVN--GNAGVL--PVSRFLGEPNDGDIKMQNQNHLLTLEQRLN 304 Query: 234 DTWTLQQNLRYTELERDETNVFPRVLLADGRTVTRLFYPRSSAWRGLQVDTRMQADFRTG 293 + W L+ + + E D + L AD T+ R R +W + + F T Sbjct: 305 ENWHLRTGIFHKEGSLDGYSTEAFALQADNATLRRQRRYRDYSWHDTSLQAEIGGKFATA 364 Query: 294 ELGHLVLLGLDYADSRTTD----RFPNSITAMPGFDLYNPVYGNNATIVRSATPRIEQYP 349 +GH VL+G + D T D R S A ++YNPVYG + T E+ Sbjct: 365 GVGHEVLVGTE-VDWLTLDQRMLRINPSNVAPYAVNIYNPVYGQAQPTPTANTDTSERQI 423 Query: 350 LRQTGLYLQDQVKWXAR 366 TGLYLQDQ+ R Sbjct: 424 --STGLYLQDQLSLSER 438 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,275,426 Number of extensions: 57278 Number of successful extensions: 164 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 129 Number of HSP's successfully gapped: 16 Length of query: 377 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 290 Effective length of database: 1,207,925 Effective search space: 350298250 Effective search space used: 350298250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)