BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0043.p (108 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 197 1e-52 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 112 5e-27 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 73 4e-15 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 73 5e-15 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 72 1e-14 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 197 bits (501), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 96/103 (93%), Positives = 100/103 (97%) Query: 6 NGXVYSASKAAVRNFARSWALDLKDRAIRVNVVSPGPIRTPGLGGLVPDEARQGLFDYLS 65 N VYSASKAAVRNFARSWALDLKDRAIRVNVVSPGPIRTPGLG LVPD+ARQGLFDYL+ Sbjct: 147 NFSVYSASKAAVRNFARSWALDLKDRAIRVNVVSPGPIRTPGLGELVPDDARQGLFDYLA 206 Query: 66 SQIPLGRLGEPQEVGKAVAFLASDAASFVNGIELFVDGGMAQV 108 SQ+PLGRLGEPQE+GKAVAFLASDAASFVNGIELFVDGGMAQV Sbjct: 207 SQVPLGRLGEPQEIGKAVAFLASDAASFVNGIELFVDGGMAQV 249 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 112 bits (281), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 60/102 (58%), Positives = 74/102 (72%), Gaps = 6/102 (5%) Query: 9 VYSASKAAVRNFARSWALDLKDRAIRVNVVSPGPIRTPGLGGLVPD--EARQGLFDYLSS 66 +Y+ASKAAVR+FARSW DLK+R IRVNVV+PG T L+ D EAR G+ D L Sbjct: 154 LYNASKAAVRSFARSWTADLKERGIRVNVVAPGGTETR----LMRDYLEARPGMEDVLKQ 209 Query: 67 QIPLGRLGEPQEVGKAVAFLASDAASFVNGIELFVDGGMAQV 108 +PLGRLGEP E+G+AV FLAS +S++ G+ELFVDGG V Sbjct: 210 VVPLGRLGEPDEIGRAVLFLASAESSYIAGVELFVDGGSVAV 251 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 73.2 bits (178), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 41/100 (41%), Positives = 54/100 (54%), Gaps = 3/100 (3%) Query: 6 NGXVYSASKAAVRNFARSWALDLKDRAIRVNVVSPGPIRTPGLGGLVPDEARQGLFDYLS 65 N Y+ASK AV N R+ ALD + +R+N V P T + D L D + Sbjct: 156 NLSAYNASKGAVCNLTRAMALDFGRKGVRINAVCPSLTHTAMTADMADDPP---LLDKFA 212 Query: 66 SQIPLGRLGEPQEVGKAVAFLASDAASFVNGIELFVDGGM 105 +I LGR +P E+ + FLAS ASFVNG+ L VDGG+ Sbjct: 213 ERIALGRGADPLEIAAVITFLASPDASFVNGVNLPVDGGL 252 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 73.2 bits (178), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 44/95 (46%), Positives = 56/95 (58%), Gaps = 3/95 (3%) Query: 10 YSASKAAVRNFARSWALDLKDRAIRVNVVSPGPIRTPGLGGLVPDEARQGLFDYLSSQIP 69 YSASK V +S A+ IRVN V+PG I TP L D R G + ++ P Sbjct: 146 YSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQDDPVRAG---PILARTP 202 Query: 70 LGRLGEPQEVGKAVAFLASDAASFVNGIELFVDGG 104 LGR G+P++VG+ VAFL S AASF+ G + VDGG Sbjct: 203 LGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGG 237 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 71.6 bits (174), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 41/101 (40%), Positives = 57/101 (56%), Gaps = 4/101 (3%) Query: 5 LNGXVYSASKAAVRNFARSWALDLKDRAIRVNVVSPGPIRTPGLGGLVPDEARQGLFDYL 64 L G YSA+KA V AR+ A + + IRVN ++PG I T ++ E + + Sbjct: 150 LGGPHYSAAKAGVLGLARAMAREFGPQGIRVNCITPGLISTDINKDILSAEKKA----EI 205 Query: 65 SSQIPLGRLGEPQEVGKAVAFLASDAASFVNGIELFVDGGM 105 + IPLGRLG P +VG FLASD + + G+ L V+GGM Sbjct: 206 AQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGVTLDVNGGM 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 353,582 Number of extensions: 14404 Number of successful extensions: 124 Number of sequences better than 1.0e-08: 23 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 23 Length of query: 108 Length of database: 1,619,870 Length adjustment: 72 Effective length of query: 36 Effective length of database: 1,278,950 Effective search space: 46042200 Effective search space used: 46042200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 124 (52.4 bits)