BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0042.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 238 4e-64 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 116 2e-27 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 114 1e-26 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 112 3e-26 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 109 3e-25 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 238 bits (607), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 120/256 (46%), Positives = 175/256 (68%), Gaps = 10/256 (3%) Query: 14 RVFISGAATGIGAAIAEAFLDTGAAVYVCDINPAAVDAIKKTHPRI-HAGVA----DVGK 68 +V ++ A GIG AI AF++ GA V++CD++ D + R HA V+ DV Sbjct: 9 KVIVTAGAQGIGLAITAAFVEAGAHVHICDVSE---DFLASARARFGHAPVSYSRTDVSS 65 Query: 69 REDVDAVMADAAEKLGG-LDLLINNAGIAGPTGAVEDLDADAWDQTINTNLNSQFYFLKR 127 +VDA+ AD A++ G LD+LINNAGIAGPT VE++ WDQT+ NL F +R Sbjct: 66 EREVDAMFADLAQRWSGRLDVLINNAGIAGPTSPVEEVALSDWDQTLAVNLTGPFLCTRR 125 Query: 128 AVPLLKNTSDNPSIITLSSVAGRLGYQFRTPYASTKWAIVGLMKSLAVELGPSNIRVNAI 187 AVPLLK +I+ +SSVAGRLG+ RTPY+++K+ ++GL ++ A+ELGPS+IRVNA+ Sbjct: 126 AVPLLKKNGGG-AIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPSHIRVNAV 184 Query: 188 LPGVVEGERMDGVIAARAKTLGLSFDEMREDYVKKISLRRLVTTQDVAAMALFLASPAAR 247 LPG+VEG R + ++AA+A G+ +EMR+ + ++SLR++VT +D+A +FL SPA Sbjct: 185 LPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQVIFLCSPAGA 244 Query: 248 NISGQAISVDGNLEYL 263 +ISGQ++SV GN+E L Sbjct: 245 SISGQSLSVCGNVEVL 260 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 116 bits (291), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 85/253 (33%), Positives = 126/253 (49%), Gaps = 20/253 (7%) Query: 16 FISGAATGIGAAIAEAFLDTGAAVYVCDINPAAVDAIKKTHPRI--HAGVA-----DVGK 68 I+G+A+GIG IA + GA V + D+ A++A K T I G A +V Sbjct: 9 LITGSASGIGKEIAVEYARQGAKVVIADL---ALEAAKATAEEIVKEGGTAMAVAMNVTD 65 Query: 69 REDVDAVMADAAEKLGGLDLLINNAGIAGPTGAVEDLDADAWDQTINTNLNSQFYFLKRA 128 VD +ADA GGLD++I+NAGI V +L + W + + +L+ F + A Sbjct: 66 ETQVDKGVADAVNTYGGLDIMISNAGIQ-IISPVAELSLENWRKMLAIHLDGAFLTTRAA 124 Query: 129 VPLLKNTSDNPSIITLSSVAGRLGYQFRTPYASTKWAIVGLMKSLAVELGPSNIRVNAIL 188 + + SII + SV L + PY + K ++GL K++A E IR N I Sbjct: 125 MKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRTNVIC 184 Query: 189 PGVVEGERMDGVIAARAKTLGLSFDEMREDYVKKISLR-----RLVTTQDVAAMALFLAS 243 PG V ++ I +AK G+S ED +KKI L+ TTQDVA A+FLA+ Sbjct: 185 PGFVRTPLVEKQIPEQAKEFGIS----EEDVIKKIMLKDTVDGEFTTTQDVAQTAVFLAA 240 Query: 244 PAARNISGQAISV 256 ++GQ++ V Sbjct: 241 FPTNALTGQSVVV 253 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 114 bits (284), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 87/254 (34%), Positives = 131/254 (51%), Gaps = 18/254 (7%) Query: 11 AGLRVFISGAATGIGAAIAEAFLDTGAAVYVCDINPAAVDAIKKTHPRIHAGVADVGKRE 70 AG V I+ AA GIG A E F GA V DI+ +D + AG+A V Sbjct: 9 AGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHLDEL--------AGIAGVETHL 60 Query: 71 -DV--DAVMADAAEKLGGLDLLINNAGIAGPTGAVEDLDADAWDQTINTNLNSQFYFLKR 127 DV DA + K+G +D+L N AG G + + D AWD + N N + F+ ++ Sbjct: 61 LDVTDDAAIKALVAKIGTIDVLFNCAGYV-AAGNILECDDKAWDFSFNLNAKAMFHTIRA 119 Query: 128 AVP--LLKNTSDNPSIITLSSVAGRL-GYQFRTPYASTKWAIVGLMKSLAVELGPSNIRV 184 +P L K SI+ ++S A + G R Y ++K A+VGL KS+A + IR Sbjct: 120 VLPGMLAKKAG---SIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRC 176 Query: 185 NAILPGVVEGERMDGVIAARAKTLGLSFDEMREDYVKKISLRRLVTTQDVAAMALFLASP 244 NAI PG +E ++ I+ +AK G S +E+R +V + + R+ ++VAA+AL+LAS Sbjct: 177 NAICPGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYLASD 236 Query: 245 AARNISGQAISVDG 258 + +G +DG Sbjct: 237 ESNFTTGSIHMIDG 250 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 112 bits (281), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 79/258 (30%), Positives = 126/258 (48%), Gaps = 17/258 (6%) Query: 8 KPDAGLRV-FISGAATGIGAAIAEAFLDTGAAVYVCDIN----PAAVDAIKKTHPRIHAG 62 + D+G RV ++G A GIGA+I++ G V V DIN DA+ R Sbjct: 3 RNDSGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPL 62 Query: 63 VADVGKREDVDAVMADAAEKLGGLDLLINNAGIAGPTGAVEDLDADAWDQTINTNLNSQF 122 D+G E + A A + G D+L+NNAG+A T + D + W QT+N N+ Sbjct: 63 QMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVA-RTYSFLDYPLENWLQTMNVNVTGVL 121 Query: 123 YFLKRAVPLLKNTSDNPSIITLSSVAGRLGYQFRTPYASTKWAIVGLMKSLAVELGPSNI 182 + A L+ I+ +SS++G RT Y ++K A++GL + +A+EL I Sbjct: 122 LAGQHAARLMVKQQWG-RIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGI 180 Query: 183 RVNAILPGVVEGERMDGVIAARAKTLGLSFDEMREDYVKKISLRRLVTTQDVAAMALFLA 242 VN++ PG V+ T + ++ RE Y + + +RR TT ++A+ +LA Sbjct: 181 TVNSVAPGPVD----------TPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLA 230 Query: 243 SPAARNISGQAISVDGNL 260 + + I+G I VDG Sbjct: 231 AEESAYITGHVIPVDGGF 248 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 109 bits (272), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 78/252 (30%), Positives = 133/252 (52%), Gaps = 16/252 (6%) Query: 12 GLRVFISGAATGIGAAIAEAFLDTGAAVYVCDINPAAVDAIKKTHP--RIHAGVADVGKR 69 G V ++GAA+GIG A A F D GA V + D + AA+ + + P R A DV Sbjct: 14 GKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARETDVSHH 73 Query: 70 EDVDAVMADAAEKLGGLDLLINNAGIAGPTGAVEDLDADAWDQTINTNLNSQFYFLKRAV 129 E V ++ A E+ G LD+L+++AG+ G V ++ + W + TN+N FY + A+ Sbjct: 74 EQVRQLVDFAIERFGQLDVLVSDAGVFA-EGNVTEVSPEDWHRVQATNVNGVFYGAREAL 132 Query: 130 PLLKNTSDNPSIITLSSVAGRLGYQFRTPYASTKWAIVGLMKSLAVELGPSNIRVNAILP 189 P L+ T I+ ++SV+G + Y ++K A+ L +++A++ G +R+NA+ P Sbjct: 133 PHLEKTRG--CIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVCP 190 Query: 190 GVVEGERMDGVIAARAKTLGLSFD-EMREDYVKKISLRRLVTTQDVAAMALFLASPAARN 248 + A T ++ D + + + ++I+L R ++AA+ FLASP A Sbjct: 191 SLTH----------TAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASF 240 Query: 249 ISGQAISVDGNL 260 ++G + VDG L Sbjct: 241 VNGVNLPVDGGL 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 689,633 Number of extensions: 24890 Number of successful extensions: 197 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 47 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)