BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0040.p (399 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 151 9e-38 gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 147 2e-36 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 145 8e-36 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 143 3e-35 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 142 5e-35 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 151 bits (382), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 106/312 (33%), Positives = 153/312 (49%), Gaps = 18/312 (5%) Query: 1 MMFVSGEWSFYILRVLLGIAEAGLFPALMYMVTLWFAGKDRAVAIGWIYTAPALALMLGN 60 M FV+ E +FY+LR LLG AEAG FP ++Y T W +R A+ + ALA +L Sbjct: 118 MAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGSALASILSG 177 Query: 61 PIGGALMQLDGMGGLHGWQWMFMIEGLPSVLLGILLYFKLPDRPGDARWLTPAEAKSLEQ 120 PI G L+Q+DG GGL GWQWMF+IEG+ SVLL ++F L P DA+WLT A+ +S Sbjct: 178 PISGGLLQIDG-GGLQGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLT-AQERSAIT 235 Query: 121 HAVIDAP------QHANTSSGDWMAALKRPTTILIGLIYFLNQVAFVGLYFFTPAMVSQM 174 A++D Q A S L+ P ++ IYF + G F+ P+++ +M Sbjct: 236 RAIVDEQAERMKHQPAQVSPRQ---LLRDPQILIFCFIYFSISLTIYGATFWLPSIIRKM 292 Query: 175 HISSPLIVGLLSASVGMGFLIGVLVLPRIHRRVSSDAVYLGVLTMGLVLCAIAFIATASF 234 S VGL ++ + ++ + + R + + A ++F Sbjct: 293 GSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFA----STF 348 Query: 235 GGKIALFVVTAFFAGGVLPS---YWSIAMKRMQGVQAAAGLAFINTIGLLGGFVGPYLFG 291 G + FV F A G + +W + + AA +A IN+IG LGGFV P FG Sbjct: 349 GNSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPTAFG 408 Query: 292 MAETATGRSNAG 303 E TG G Sbjct: 409 FLEQRTGSIQGG 420 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 147 bits (370), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 105/315 (33%), Positives = 158/315 (50%), Gaps = 24/315 (7%) Query: 1 MMFVSGEWSFYILRVLLGIAEAGLFPALMYMVTLWFAGKDRAVAIGWIYTAPALALMLGN 60 M FV E SFY +R LLG+AEAG FP ++Y T W +R A+ + A+A +L Sbjct: 106 MAFVQSETSFYFMRFLLGVAEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSG 165 Query: 61 PIGGALMQLDGMGGLHGWQWMFMIEGLPSVLLGILLYFKLPDRPGDARWLTPAEAKSLEQ 120 PI GAL+ ++G GGLHGWQWMF+IEG S++ ++++ L P +A+WL+ + ++L Sbjct: 166 PITGALLLIEG-GGLHGWQWMFIIEGGASMVFCVVVWLVLDSTPREAKWLSQEQREALA- 223 Query: 121 HAVIDAPQHANTSS----GDWMAALKRPTTILIGLIYFLNQVAFVGLYFFTPAMVSQMHI 176 AVI A Q ++ ++ L+ P +L IYF Q+ G F+ P ++ +M Sbjct: 224 -AVIAAEQEQRAANRPAHVSALSLLRDPQIVLFCFIYFAIQLTIYGATFWLPTIIKKMGH 282 Query: 177 SSPLIVGLLSA-----SVGMGFLIGVLVLPRIHRRVSSDAVYLGVLTMGLVLCAIAFIAT 231 + VGL ++ ++ +L VL R + A L + G+ + A+A Sbjct: 283 FNDFQVGLFNSIPWIVAIAAMYLFAVLA-ARYKKPQFWVATTLVISAAGMAISAVA---- 337 Query: 232 ASFGGKIALFVVTAFFAGGVLPS---YWSIAMKRMQGVQAAAGLAFINTIGLLGGFVGPY 288 G + FV F G + +W I + AAA LA IN++G LGGF P Sbjct: 338 ----GPVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARIAAAVLALINSLGNLGGFFAPA 393 Query: 289 LFGMAETATGRSNAG 303 FG E TG G Sbjct: 394 TFGYLEQKTGSIQGG 408 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 145 bits (365), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 104/313 (33%), Positives = 156/313 (49%), Gaps = 21/313 (6%) Query: 1 MMFVSGEWSFYILRVLLGIAEAGLFPALMYMVTLWFAGKDRAVAIGWIYTAPALALMLGN 60 M +V+ FYI+R LLG+AEAG FP ++ +T W+ R T AL+ ++G Sbjct: 109 MAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGG 168 Query: 61 PIGGALMQ-LDGMGGLHGWQWMFMIEGLPSVLLGILLYFKLPDRPGDARWLTPAEAKSLE 119 P+ G +M + G+ G GWQWMF++E +PS++LG+++ + DR DA WL+ E + LE Sbjct: 169 PLSGWIMHAMPGVWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLE 228 Query: 120 QHAVIDAPQHANTSSGDWMAALKRPTTILIGLIYFLNQVAFVGLYFFTPAMVSQMHISSP 179 + + Q + S G A L LIYF + G+ F+ P ++ +S P Sbjct: 229 ANISHEESQKEHLSLG---AMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDP 285 Query: 180 LIVGLLSA-----SVGMGFLIGVLVLPRIHRRVSSDAVYLGVLTMGLVLCAIAFIATASF 234 L VGLL+A + LIG R RR V + C I + + + Sbjct: 286 LNVGLLTAIPYAFAAAAMVLIGRSADARRERRWH-------VAIPAFIGC-IGLLLSTQY 337 Query: 235 GGKIALFVVTAFFAG----GVLPSYWSIAMKRMQGVQAAAGLAFINTIGLLGGFVGPYLF 290 G L + + A LP +WS+ + G AAAG+A IN++G L GFV P+L Sbjct: 338 GHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLV 397 Query: 291 GMAETATGRSNAG 303 G + AT +NAG Sbjct: 398 GWLKDATQSTNAG 410 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 143 bits (360), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 104/320 (32%), Positives = 168/320 (52%), Gaps = 34/320 (10%) Query: 1 MMFVSGEWSFYILRVLLGIAEAGLFPALMYMVTLWFAGKDRAVAIGWIYTAPALALMLGN 60 M +V+ SFYILR LLGI EAG FP ++ +T WF + RA G T+ A+A +G Sbjct: 113 MAYVTTPTSFYILRFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGG 172 Query: 61 PIGGALMQ-LDGMGGLHGWQWMFMIEGLPSVLLGILLYFKLPDRPGDARWLTPAEAKSLE 119 PI G +M ++G+G L WQW+F++EG+PS+L G + LP+RP +A+WL+ AE ++ Sbjct: 173 PIAGLIMSGMEGVGHLANWQWLFILEGIPSILAGFAVLAWLPERPANAKWLSEAEKNAVT 232 Query: 120 QHAVIDAPQHANTSSGDWMAALKRPTTILIGLIYFLNQVAFVGLYFFTPAMVSQMHISSP 179 Q V+ A A + A + +YF + F+ P+++ ++ + Sbjct: 233 Q--VLAAENSAPGKHFSFKDACTNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGN 290 Query: 180 LIVGLLSASVGMGFLIGVLVLP------------RIHRRVSSDAVYLGVLTMGLVL---- 223 L +GL+SA + F+ G L + R+H +++ +G++ G+ L Sbjct: 291 LQIGLISA---IPFIAGTLAMIWNGFHSDKTGERRLHSAIATLIACIGLVATGMTLGNAT 347 Query: 224 CAIAFIATASFGGKIALFVVTAFFAGGVLPSYWSIAMKRMQGVQAAAGLAFINTIGLLGG 283 A+ + A+ G ++ AF P +WSI + G AA G+A IN+IG L G Sbjct: 348 LALCSLTLAAIG------ILAAF------PVFWSIPGAFLAGTAAAGGIALINSIGNLAG 395 Query: 284 FVGPYLFGMAETATGRSNAG 303 FV PY+ G +T+TG ++G Sbjct: 396 FVAPYMIGALKTSTGSLSSG 415 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 142 bits (358), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 103/307 (33%), Positives = 157/307 (51%), Gaps = 7/307 (2%) Query: 1 MMFVSGEWSFYILRVLLGIAEAGLFPALMYMVTLWFAGKDRAVAIGWIYTAPALALMLGN 60 MM V+ FY LR LLG+ EAG FP ++ ++ WF + RA + + A+A +LG Sbjct: 113 MMAVTDAAHFYTLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGG 172 Query: 61 PIGGALMQ-LDGMGGLHGWQWMFMIEGLPSVLLGILLYFKLPDRPGDARWLTPAEAKSLE 119 + G +M+ + G+ GL+GWQWMF IEG P+V+LG+L F L D P A+WL+P E L+ Sbjct: 173 LVSGWIMRDMAGVMGLYGWQWMFAIEGAPAVVLGLLAIFLLADGPAQAKWLSPEERSFLQ 232 Query: 120 QHAVIDAPQHANTSSGDWMAALKRP----TTILIGLIYFLNQVAFVGLYFFTPAMVSQMH 175 +A + + SG +AAL++ TT L IYF + L F+ P M+ Sbjct: 233 ARHQQEALRTHHV-SGHSLAALRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFG 291 Query: 176 ISSPLIVGLLSA-SVGMGFLIGVLVLPRIHRRVSSDAVYLGVLTMGLVLCAIAFIATASF 234 I + V L S +G + +L+ R RR + T G + A + +SF Sbjct: 292 IKDVVEVSLYSVIPNAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSF 351 Query: 235 GGKIALFVVTAFFAGGVLPSYWSIAMKRMQGVQAAAGLAFINTIGLLGGFVGPYLFGMAE 294 + L V A LP +WS+ + A G+AFI+++G+ G V P+L G+ + Sbjct: 352 AVSLGLLSVAAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVK 411 Query: 295 TATGRSN 301 T TG + Sbjct: 412 TRTGNMD 418 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.457 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,353,329 Number of extensions: 60303 Number of successful extensions: 349 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 327 Number of HSP's successfully gapped: 14 Length of query: 399 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 312 Effective length of database: 1,207,925 Effective search space: 376872600 Effective search space used: 376872600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)