BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0020.p (1105 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 264 4e-71 gi|300313029|ref|YP_003777121.1| two component hybrid sensor his... 226 6e-60 gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 214 3e-56 gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729... 191 3e-49 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 183 7e-47 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 264 bits (674), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 183/541 (33%), Positives = 278/541 (51%), Gaps = 68/541 (12%) Query: 395 ELHQAKEQALTATRAKSEFLANMSHEIRTPMNAIIGMSHLALQTSLDKRQRNYIEKVQRA 454 +L QAKE A A + FL+ MSHEIRTPMNA++GM+ L T LD +Q + + ++ + Sbjct: 72 KLVQAKEAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAGLLRSTRLDTQQAEFAKAIEDS 131 Query: 455 GENLLGIINDILDFSKIEAGKLTVEQIDFSLEDVMDNLVSLIGLKAEDKGLELLFQIRSD 514 LLGI+ND+LDFSKI+ GK+ + ++F L V++ + KA +K + LL + S Sbjct: 132 AHALLGIVNDVLDFSKIDVGKMDIAVVEFDLAKVVEASAEALSFKAREKRVRLLAHLDSR 191 Query: 515 VPGDLIGDPLRIGQVLVNLGTNAVKFTDAGEIVVAVERISGDDDSVMLHFSVKDSGIGMT 574 +P L+GDP RI Q+L+NL NA+KFT AGE+++ V+ + D + F V+DSGIGM Sbjct: 192 LPPRLVGDPGRIRQILLNLLGNALKFTHAGEVLLRVQALQVTSDHCRVRFEVRDSGIGMD 251 Query: 575 EEQCDKLFQSFSQADASTTRKYGGSGLGLAISKNLLELMDGRIWVESEIGRGSTFHCHAR 634 E+ +LF F+QAD+S TR+YGG+GLGL+I K L+ELM G I VES +G GS F Sbjct: 252 EDTLRRLFSPFAQADSSVTRQYGGTGLGLSICKRLVELMGGSIGVESALGTGSCFWFE-- 309 Query: 635 FGLQKNAKPRRMFHADELLG---IRVLVVDDNAMAREILSTMARNFGLEVDVASGGAQGV 691 G+ P M ++ R+ +V+ + + +L R+ LE + + Sbjct: 310 LGIPVTTGP-GMIRSEPPAADRPRRIALVEPHDASAAMLHEYLRSAALETLRFGSASDAL 368 Query: 692 EMVLQAERQSLPYNLMLMDWKMPGMDGIATVQRLRATSLAQTPAVIMVTAYGREDALLNA 751 + + + +++ ++ +D ++ + RL+ A+ PA+ V LL+ Sbjct: 369 AALTGPGASASGIDAVIVSTQVQDLDAVSVMARLQ----AERPALRRV--------LLDH 416 Query: 752 AEQGVELSS-----VLTKPVTSSSLLEAIGLALGKGTLVETRASERGXWAG--------D 798 G++ S VL P+ LL A+G+ + E + S A Sbjct: 417 GVHGLDTGSKPHHEVLQLPLRKQQLLRALGV---TDSAAEAQTSPHARMAAPATPRQGHS 473 Query: 799 GQQ------------------------AVDI-------LARDARFDGVL--MDCQMPVMD 825 GQ+ A DI L R AR L MDCQMP++D Sbjct: 474 GQRDILLVEDNVMNQKVAVYQLNAMGYAADIAANGLEALERLARHRYALVLMDCQMPLLD 533 Query: 826 GYEAT-RVLRGNPSFSGMPIIAMTANVMMSDKQRALDVGMQDHIAKPLNVGDMFATLARW 884 GYE T R+ R + I+AMTAN M D++R L+ GM D++ KPL + A LA + Sbjct: 534 GYETTRRIRRMQGELAHTTIVAMTANAMAGDRERCLEAGMDDYLTKPLLRSQLSAMLAHY 593 Query: 885 I 885 + Sbjct: 594 L 594 >gi|300313029|ref|YP_003777121.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1524 Score = 226 bits (577), Expect = 6e-60, Method: Compositional matrix adjust. Identities = 180/555 (32%), Positives = 282/555 (50%), Gaps = 68/555 (12%) Query: 261 VALDQTKSGIWVVDFKDPEHVFMPERTARLLGEEIKPDGGLYRLDK--EWFERVVDADKT 318 +A + K G+WV D KD + + + A + G GG R+ +W RV Sbjct: 761 LAAEVAKLGVWVWD-KDSDVLEYNPQVAEIYG--FGDRGGAPRVSTYADWRRRV------ 811 Query: 319 LGPDQACAELTNSRYRAAISGETEKYESIYAYKRPVDGEVIWLHAH--------GRPVRD 370 PD R A+ G+ +E ++ R DG + ++ A GRPVR Sbjct: 812 -HPDDVDYVEKTLRAGLAVDGD---HEPVFRVVRD-DGGIRYVQARAYVQRDGAGRPVR- 865 Query: 371 ERTQEILFIYGAYLDITAQKNAENELHQAKEQALTATRAKSEFLANMSHEIRTPMNAIIG 430 + LDIT + EN L A+ +A A AKS FLA++SHEIRTPMN ++G Sbjct: 866 --------VICINLDITQRIEFENNLVDARLKAEQANSAKSLFLASVSHEIRTPMNGVLG 917 Query: 431 MSHLALQTSLDKRQRNYIEKVQRAGENLLGIINDILDFSKIEAGKLTVEQIDFSLEDVMD 490 M L +T Q++Y++K + + ++LL ++ND+LDFSK++A +L ++ F LE Sbjct: 918 MIQLLQRTQPLPAQQDYLDKAEISAKSLLNLLNDLLDFSKMDAKQLQLDLQAFELERFGH 977 Query: 491 NLVSLIGLKAEDKGLELLFQIRSDVPGDLIGDPLRIGQVLVNLGTNAVKFTDAGEIVVAV 550 NL +++ G+ L + +P L+GD R+ QVLVNL NA+KFT G I V Sbjct: 978 NLAAVLAGSPPQAGVSLHIALDPQLPRRLVGDAHRLQQVLVNLTGNALKFTHQGHIDVHF 1037 Query: 551 ERISG-DDDSVMLHFSVKDSGIGMTEEQCDKLFQSFSQADASTTRKYGGSGLGLAISKNL 609 R+ +V L V D+GIG+ ++ D +F +F+QA+ASTTR+YGG+GLGLAISK+L Sbjct: 1038 TRLDPLPGRTVALRVEVSDTGIGIAADRLDDIFDAFTQAEASTTRRYGGTGLGLAISKHL 1097 Query: 610 LELMDGRIWVESEIGRGSTFHCHARFGLQKNA-----------KP--------------- 643 + LM G++ V SE GRGS F + + ++ +P Sbjct: 1098 VGLMGGQLQVSSEPGRGSRFWFDLQLAVDDDSVLGPAAADDAARPQPLQAASHTAGSRAP 1157 Query: 644 ---RRMFHADELLGIRVLVVDDNAMAREILSTMARNFGLEVDVASGGAQGVEMVLQAERQ 700 R A L G+++L+V+DN + R++ S + G +V A G+ GV M R Sbjct: 1158 ETARSAPDAQCLQGVQILLVEDNPVNRQVASELLALSGAQVTCADSGSMGVAMACDPARH 1217 Query: 701 SLPYNLMLMDWKMPGMDGIATVQRLRATSLAQTPAVIMVTAYGREDALLNAAEQGVELSS 760 ++L+LMD +MPGMDGI +R+R + ++ +TA + E G ++ Sbjct: 1218 ---FDLVLMDLQMPGMDGIEATRRIRGQYELRGLPILAMTANASPEDKQRCIEAG--MAG 1272 Query: 761 VLTKPVTSSSLLEAI 775 + KP+ ++L+ + Sbjct: 1273 HIAKPIDIATLIPVL 1287 Score = 94.4 bits (233), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 78/256 (30%), Positives = 113/256 (44%), Gaps = 43/256 (16%) Query: 754 QGVELSSVLTKPVTSSSLLEAIGLALGKGTLVETRASERGXWAGDGQQAVDILARDAR-F 812 QGV++ V PV E + L+ + T ++ G V + AR F Sbjct: 1170 QGVQILLVEDNPVNRQVASELLALSGAQVTCADS-----------GSMGVAMACDPARHF 1218 Query: 813 DGVLMDCQMPVMDGYEATRVLRGNPSFSGMPIIAMTANVMMSDKQRALDVGMQDHIAKPL 872 D VLMD QMP MDG EATR +RG G+PI+AMTAN DKQR ++ GM HIAKP+ Sbjct: 1219 DLVLMDLQMPGMDGIEATRRIRGQYELRGLPILAMTANASPEDKQRCIEAGMAGHIAKPI 1278 Query: 873 NVGDMFATLARWIHPQEPGIHTLSARTVTPE---KGMPPVI--DSIDGIDIQAGLATTMN 927 ++ + L +H + P PE + +P + D + D + L Sbjct: 1279 DIATLIPVLLAHLHHERP----------VPEAAPQAVPQDLADDRLSVEDYSSILHRFQQ 1328 Query: 928 NLPLYR-----------RLLSKFRTGQGNFSQMFEAACRDADASAPARLAHTLKGTAGNI 976 +YR RLL+++ + + A +A A+ +H L+G I Sbjct: 1329 ASDIYRTTLDAAGPELERLLAQYCAALAMSPEQAQEAM-----AALAQASHALRGVTATI 1383 Query: 977 GAREVQAAAAELETAC 992 GAR + A LE C Sbjct: 1384 GARGLAAYFGALEQRC 1399 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 214 bits (546), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 139/397 (35%), Positives = 229/397 (57%), Gaps = 20/397 (5%) Query: 385 DITAQKNAENELHQAKEQALTATRAKSEFLANMSHEIRTPMNAIIGMSH-LALQTSLDKR 443 +I A K + EL +AKE A +A AKS F+A MSHE+RTP+N+I+G + L + ++ Sbjct: 663 EIEAHKQTDAELQRAKEAAESANLAKSRFVAGMSHELRTPLNSILGYAQILHVDATIPPA 722 Query: 444 QRNYIEKVQRAGENLLGIINDILDFSKIEAGKLTVEQIDFSLEDVMDNLVSLIGLKAEDK 503 +++ I ++R+GE+LL +I+ +LD +KIEAGK+ + + +LE ++ +V + +AE K Sbjct: 723 RKDAIGVIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELALEAFLEQIVGMFAPQAEQK 782 Query: 504 GLELLFQIRSDVPGDLIGDPLRIGQVLVNLGTNAVKFTDAGEIVVAVERISGDDDSVMLH 563 GL F +P + D R+ Q+L+NL NAVKFTD G + + + R + + + + Sbjct: 783 GLHFRFDKSGRLPQIVHADQKRLRQILINLLGNAVKFTDHGGVTLRL-RYARE----IAY 837 Query: 564 FSVKDSGIGMTEEQCDKLFQSFSQADASTTRKYGGSGLGLAISKNLLELMDGRIWVESEI 623 F V D+GIG+ ++ +++F F ++ A+ R+ G+GLGLAIS+ L+ +M G + V+S++ Sbjct: 838 FDVIDTGIGIADDDRERIFLPFERSSAANLREDIGTGLGLAISRMLVHIMGGELSVQSQV 897 Query: 624 GRGSTFHCHARFGLQKNAKPRRMFHADELLGI-----RVLVVDDNAMAREILSTMARNFG 678 G+GS+FH +PR D++ G +LVVDD A R +L M G Sbjct: 898 GQGSSFHLKIYLPEVHVPRPRIKLE-DQMSGYAGPSRHILVVDDQASQRRLLKDMLMPLG 956 Query: 679 LEVDVASGGAQGVEMVLQAERQSLPYNLMLMDWKMPGMDGIATVQRLRATSLAQTPAVIM 738 V A G +E + A LP +L+L+D MP MDG + + +RA LAQ P +++ Sbjct: 957 FTVSEADSGLACMETLGHA----LP-DLVLLDIAMPQMDGWSVARAIRARGLAQLP-ILL 1010 Query: 739 VTAYGREDALLNAAEQGVELSSVLTKPVTSSSLLEAI 775 V+A E+ L+ + ++ + KPV+ LL I Sbjct: 1011 VSANAFEN--LHERNEPPLVNDFVVKPVSYHELLGKI 1045 >gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729 [Herbaspirillum seropedicae SmR1] Length = 765 Score = 191 bits (485), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 106/236 (44%), Positives = 154/236 (65%), Gaps = 10/236 (4%) Query: 400 KEQALTATRAKSEFLANMSHEIRTPMNAIIGMSHLALQTSLDKRQRNYIEKVQRAGENLL 459 KE+A A AKS FLA +SHEIRTPMN I+G+S L +++ L +QR+Y + + A L+ Sbjct: 338 KEEADHANEAKSHFLAMVSHEIRTPMNGILGLSELLMESGLSSKQRDYADSIHGATGGLV 397 Query: 460 GIINDILDFSKIEAGKLTVEQIDFSLEDVMDNLVSLIGLKAEDKGLELLFQIRSDVPGDL 519 IINDILDFSKIE+GKL +E + F ++ L L + K ++ F + D P +L Sbjct: 398 RIINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPSLQRKDVQ--FDLHLDCPENL 455 Query: 520 --IGDPLRIGQVLVNLGTNAVKFTDAGEIVVAVERISGDDDS----VMLHFSVKDSGIGM 573 +GDP R+ QVL NL +NA+KFT AG + +++ + D+S L F++ D+GIG+ Sbjct: 456 SVVGDPARLKQVLGNLLSNAIKFTQAGHVQLSMS--ARQDESGPSRWRLFFAITDTGIGI 513 Query: 574 TEEQCDKLFQSFSQADASTTRKYGGSGLGLAISKNLLELMDGRIWVESEIGRGSTF 629 + E +LF+ F+QAD+S +R++GG+GLGLAISKNL+ELM G I +S G + F Sbjct: 514 SPEALQQLFRPFAQADSSISRRFGGTGLGLAISKNLVELMGGTIACQSIPGESTRF 569 Score = 82.8 bits (203), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 39/92 (42%), Positives = 60/92 (65%), Gaps = 4/92 (4%) Query: 798 DGQQAVDILARDARFDGVLMDCQMPVMDGYEATRVLRGNPSFSGMP---IIAMTANVMMS 854 +G QAV+ + R +D VLMDC MP+MDGY+AT +R + + G+P IIA+TA+ + Sbjct: 673 NGLQAVEAI-RTGHYDLVLMDCMMPIMDGYQATAEIRAHEAARGLPRTPIIALTASAIEG 731 Query: 855 DKQRALDVGMQDHIAKPLNVGDMFATLARWIH 886 D+QR LD GM D+++KP + T+ +W+ Sbjct: 732 DRQRCLDAGMDDYLSKPFTQVGLMQTVEKWLQ 763 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 183 bits (464), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 98/227 (43%), Positives = 144/227 (63%), Gaps = 7/227 (3%) Query: 406 ATRAKSEFLANMSHEIRTPMNAIIGMSHLAL---QTSLDKRQRNYIEKVQRAGENLLGII 462 A + KSEFLANMSHE+RTP+N+ + ++ L Q +L Q + + + AG +LL +I Sbjct: 471 ANQYKSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQVRFAQTISSAGNDLLALI 530 Query: 463 NDILDFSKIEAGKLTVEQIDFSLEDVMDNLVSLIGLKAEDKGLELLFQIRSDVPGDLIGD 522 NDILD SK+EAGKL + F L + D L + + A+ KGL ++ VP + D Sbjct: 531 NDILDLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGLGFKVEVEPGVPQRMETD 590 Query: 523 PLRIGQVLVNLGTNAVKFTDAGEIVVAVERISGDDDSVMLHFSVKDSGIGMTEEQCDKLF 582 R+GQ+L NL +NA+KFT+ GE+ + + +G + F+V+D+GIG+ E+Q +F Sbjct: 591 IQRLGQILKNLLSNAIKFTEKGEVSLRIYPDAGG----RIAFAVRDTGIGIGEDQHQFIF 646 Query: 583 QSFSQADASTTRKYGGSGLGLAISKNLLELMDGRIWVESEIGRGSTF 629 ++F QAD ST RKYGG+GLGL+IS++L L+ G I V S +G GS F Sbjct: 647 EAFRQADGSTHRKYGGTGLGLSISRDLAHLLGGDIRVTSRLGEGSIF 693 Score = 71.6 bits (174), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 38/95 (40%), Positives = 55/95 (57%), Gaps = 5/95 (5%) Query: 796 AGDGQQAVDILAR-----DARFDGVLMDCQMPVMDGYEATRVLRGNPSFSGMPIIAMTAN 850 A +G++A+ L R DA D VLMD MP MDG A R +R + +PIIA+TA Sbjct: 1064 ARNGREALQALQRSQDKPDAPIDLVLMDIMMPEMDGLTAMREIRKQAVWKRLPIIALTAK 1123 Query: 851 VMMSDKQRALDVGMQDHIAKPLNVGDMFATLARWI 885 M D+++ L G D+IAKPL+V + + + W+ Sbjct: 1124 AMKDDQEKCLAAGANDYIAKPLDVEKLLSLVRVWM 1158 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,177,237 Number of extensions: 132472 Number of successful extensions: 818 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 798 Number of HSP's successfully gapped: 48 Length of query: 1105 Length of database: 1,619,870 Length adjustment: 95 Effective length of query: 1010 Effective length of database: 1,170,045 Effective search space: 1181745450 Effective search space used: 1181745450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 136 (57.0 bits)