BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0004.p (266 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310325|ref|YP_003774417.1| metal ion ABC transporter perip... 445 e-126 gi|300314030|ref|YP_003778122.1| metal ion ABC transporter perip... 292 2e-80 gi|300311120|ref|YP_003775212.1| metal ion ABC transporter perip... 273 1e-74 gi|300310887|ref|YP_003774979.1| metal ion ABC transporter perip... 213 1e-56 gi|300311637|ref|YP_003775729.1| metal ion ABC transporter perip... 159 3e-40 >gi|300310325|ref|YP_003774417.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 265 Score = 445 bits (1144), Expect = e-126, Method: Compositional matrix adjust. Identities = 221/266 (83%), Positives = 248/266 (93%), Gaps = 1/266 (0%) Query: 1 MNRRQLIQFFAGLGVVAGLVSAPVLAQDKPIKIGATGGPHAEILEQVKKLAEKDGLKLQI 60 M RRQLIQF AGLG+ AGLVSAP AQD+ IK+G T GPHAEI+EQVKKL EKDG+++++ Sbjct: 1 MKRRQLIQFIAGLGLAAGLVSAPAFAQDQ-IKMGVTAGPHAEIMEQVKKLLEKDGVQMKV 59 Query: 61 VEFSDYIQPNAALAAGDLDANSYQHQPYLDAQVKDRGYKLVSVGTTITFPMGVYSKKIKS 120 +EF+DYIQPNAALAAGDLDANSYQHQPYLDAQ+KDRGYK VSVG+TITFPMGVYSKKIKS Sbjct: 60 IEFTDYIQPNAALAAGDLDANSYQHQPYLDAQIKDRGYKFVSVGSTITFPMGVYSKKIKS 119 Query: 121 LKDLKNGARVGIPNDPTNGGRALLLLQAQGVIKLKADAGLKATPLDIVDNPKKVKIIELD 180 L DLK GARVG+PNDPTNGGRALL+LQA+GVIKLKADAGLKATPLDIV+NPKK+KI+ELD Sbjct: 120 LNDLKQGARVGVPNDPTNGGRALLVLQAKGVIKLKADAGLKATPLDIVENPKKIKIVELD 179 Query: 181 AAQLPRSLDDFDAAAINGNFAESAGLSPTKDGIAIEAAKGPYVNVIAVRVEDKDKPWVAK 240 AAQLPRSLDDFDAA INGN+AESAGLSPTKD IA+EA+ GPY NVIAVR+ DKDKPWVAK Sbjct: 180 AAQLPRSLDDFDAAVINGNYAESAGLSPTKDAIAVEASTGPYANVIAVRIADKDKPWVAK 239 Query: 241 LIKAYHSPEIKKFVIEKYKNSVVTSW 266 L+KAYHSPE+KKFV+EKYK SV+TSW Sbjct: 240 LVKAYHSPEVKKFVLEKYKGSVITSW 265 >gi|300314030|ref|YP_003778122.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 276 Score = 292 bits (747), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 133/260 (51%), Positives = 192/260 (73%), Gaps = 6/260 (2%) Query: 13 LGVVAGLVSAPVLAQDKPIKIGATGGPHAEILEQVKKLAEKD--GLKLQIVEFSDYIQPN 70 + ++A LV A D+ I++G T GPHA+I EQ +++ E+D G K++I+EF+DYIQPN Sbjct: 17 VSMLASLVLNAQAAADQVIRVGVTSGPHAQIFEQARRVFERDNPGYKVKIIEFNDYIQPN 76 Query: 71 AALAAGDLDANSYQHQPYLDAQVKDRGYKLVSVGTTITFPMGVYSKKIKSLKDLKNGARV 130 AAL AG+LDANSYQH+P+L+AQ+K RGYKL + G T+ PM +YS+K + L+D+ GAR+ Sbjct: 77 AALDAGELDANSYQHRPFLNAQIKTRGYKLYAEGKTMIGPMAIYSRKYRKLEDVPVGARI 136 Query: 131 GIPNDPTNGGRALLLLQAQGVIKLKAD----AGLKATPLDIVDNPKKVKIIELDAAQLPR 186 GIPNDP N R LLLLQ G IKL+A G ATP+DI +NPKK K +E+DAAQLPR Sbjct: 137 GIPNDPANESRVLLLLQKHGFIKLRAGIDPLTGTNATPIDITENPKKWKFVEIDAAQLPR 196 Query: 187 SLDDFDAAAINGNFAESAGLSPTKDGIAIEAAKGPYVNVIAVRVEDKDKPWVAKLIKAYH 246 +LDD DA+A+N ++A AGL+P +D + +E+ PY +IAVR +D+ +PW+ KL++AY Sbjct: 197 TLDDLDASAVNADYAAKAGLNPARDSLVVESGDSPYACLIAVREKDRAQPWLGKLVQAYQ 256 Query: 247 SPEIKKFVIEKYKNSVVTSW 266 SPE+K+++ ++K ++ +W Sbjct: 257 SPEVKRYIETEFKGGILAAW 276 >gi|300311120|ref|YP_003775212.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 273 bits (698), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 146/266 (54%), Positives = 202/266 (75%), Gaps = 7/266 (2%) Query: 7 IQFFAGLGVVAGLVSAPVL--AQDKP-IKIGATGGPHAEILEQVKKLAEKDGLKLQIVEF 63 I+ A +G ++ L P L AQDK IK+G + G I + VKK+A +DGL++Q V F Sbjct: 10 IKRLAAIGALS-LALVPQLGQAQDKQKIKVGISVGSAEAIFDVVKKVAARDGLEIQTVVF 68 Query: 64 SDYIQPNAALAAGDLDANSYQHQPYLDAQVKDRGYKLVSVGTTITFPMGVYSKKIKSLKD 123 SDY+QPNAALAAGDLDAN++QH+PYL++Q+ RGY++V VG TIT P+G+YS+K +S++ Sbjct: 69 SDYLQPNAALAAGDLDANAFQHRPYLESQIAARGYRIVPVGLTITAPLGIYSRKFRSVEQ 128 Query: 124 LKNGARVGIPNDPTNGGRALLLLQAQGVIKLK---ADAGLKATPLDIVDNPKKVKIIELD 180 L GA +GI NDP+NG RALLLLQ G+I+LK + G+ ATPLD+++NP+K+K++ELD Sbjct: 129 LPQGAAIGIQNDPSNGNRALLLLQKAGLIRLKPGVGENGVNATPLDVIENPRKLKLVELD 188 Query: 181 AAQLPRSLDDFDAAAINGNFAESAGLSPTKDGIAIEAAKGPYVNVIAVRVEDKDKPWVAK 240 AAQLPRSLDD AA+IN ++A AGLS KD IA+E +G Y N+IA R EDKD+PWV K Sbjct: 189 AAQLPRSLDDLAAASINNDYAFKAGLSLQKDTIAVEDPRGRYANIIATRAEDKDRPWVRK 248 Query: 241 LIKAYHSPEIKKFVIEKYKNSVVTSW 266 L++AYHS E++KF+ ++K S+V ++ Sbjct: 249 LVQAYHSEEVRKFIETEFKGSLVPAF 274 >gi|300310887|ref|YP_003774979.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 213 bits (542), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 118/267 (44%), Positives = 160/267 (59%), Gaps = 5/267 (1%) Query: 1 MNRRQLIQFFAGLGVVAGLVSAPVLAQDKPIKIGATGGPHAEILEQVKKLAEKDGLKLQI 60 M R+ + FAG + S A DK + I AT PHAEILE +K K+G+ LQ+ Sbjct: 1 MIRKLIPHLFAGAALA---FSVGAHAADK-LVIAATPVPHAEILEHIKPALAKEGIDLQV 56 Query: 61 VEFSDYIQPNAALAAGDLDANSYQHQPYLDAQVKDRGYKL-VSVGTTITFPMGVYSKKIK 119 F+DY+QP A +D N + HQPYLD K + V V P YS+K K Sbjct: 57 KVFTDYVQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKYK 116 Query: 120 SLKDLKNGARVGIPNDPTNGGRALLLLQAQGVIKLKADAGLKATPLDIVDNPKKVKIIEL 179 + D+ NGA + IPNDP+N GRALLLL G+IKLK + + AT DIV+NPKK+K L Sbjct: 117 KIADIPNGATIAIPNDPSNSGRALLLLARNGLIKLKDNTNISATQKDIVENPKKLKFRAL 176 Query: 180 DAAQLPRSLDDFDAAAINGNFAESAGLSPTKDGIAIEAAKGPYVNVIAVRVEDKDKPWVA 239 +AA LPR L+ D A IN N+A A L+P KD + IE A PY N++ R ++KD P + Sbjct: 177 EAATLPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSPAIK 236 Query: 240 KLIKAYHSPEIKKFVIEKYKNSVVTSW 266 KL A +SP++KKF+ EKY+ ++V ++ Sbjct: 237 KLAAALNSPDVKKFIEEKYQGAIVPAF 263 >gi|300311637|ref|YP_003775729.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 159 bits (401), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 95/225 (42%), Positives = 130/225 (57%), Gaps = 6/225 (2%) Query: 26 AQDKPIKIGATGGPHAEILEQVKKLAEKDGLKLQIVEFSDYIQPNAALAAGDLDANSYQH 85 A DK ++IG G + + + K A++ G+ +Q+VEF+D+ PN AL AGDLD N +QH Sbjct: 21 AADK-LRIGVVPGAYGDSVAVAAKEAKRQGIDVQVVEFTDWTTPNVALDAGDLDLNYFQH 79 Query: 86 QPYLDAQVKDRGYKLVSVGTTITFPMGVYSKKIKSLKDLKNGARVGIPNDPTNGGRALLL 145 QP+LD ++ GYKL S GT I +G+YS K KSL L GA+VGI NDP N GRALLL Sbjct: 80 QPFLDNAIQKNGYKLASAGTGILSNVGLYSLKYKSLTALPQGAKVGIANDPVNQGRALLL 139 Query: 146 LQAQGVIKLKADAGLKATPLDIVDNPKKVKIIELDAAQLPRSLDDFDAAAINGNFAESAG 205 LQ G+IKLK G + DI+DNP+K+ +E++ QL R D D A +F +A Sbjct: 140 LQNVGLIKLKPKVGYLGSIDDIIDNPRKLSFVEVEGPQLVRITPDVDLAQGYPHFIVAAK 199 Query: 206 LSPTKDGIA---IEAAKGPYVNVIAVRVEDKDKPWVAKLIKAYHS 247 G+A IE A+ + R + P V K I+ Y + Sbjct: 200 AFDPSSGLAYSGIEDAR--FAIQFVTRANRVNDPVVQKFIQIYQN 242 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.135 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 764,666 Number of extensions: 29985 Number of successful extensions: 119 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 5 Length of query: 266 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 182 Effective length of database: 1,222,130 Effective search space: 222427660 Effective search space used: 222427660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 130 (54.7 bits)