BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0001.p (159 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 118 2e-28 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 118 bits (296), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 63/91 (69%), Positives = 72/91 (79%), Gaps = 1/91 (1%) Query: 1 VGPLVGGILIHSFGWRSVFLINVPVGLLGLFMAVKMIPAVERHMRALTITSHFLGVLMLA 60 VGPLVGG+LIH GWRSVFLINVPVGL+GL MA K+IP V H RAL ++SH GV+MLA Sbjct: 170 VGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQVGGHARALNLSSHLAGVVMLA 229 Query: 61 ALSFVLIQGPVYGWTSLPIVTAVIVVVLALG 91 LSFVLIQGPVYGWTS P + A + V L+ G Sbjct: 230 GLSFVLIQGPVYGWTS-PRILAGLAVALSAG 259 Score = 58.2 bits (139), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 55/150 (36%), Positives = 74/150 (49%), Gaps = 25/150 (16%) Query: 2 GPLVGGILIHSFGWRSVFLINVPVGLLGLFMAVKMIPAVERHMRALTITSHFLGVLMLAA 61 G L+ G + +G R L+ + VG + +A+ PA + AL S LG+ Sbjct: 334 GNLMSGRMSAHWGPRVPLLLGLSVGTVFTALAMGFSPATPYWLLALVCASANLGIS---- 389 Query: 62 LSFVLIQGPVYGWTSLPIVTAVIVVV--------LALGMLVQRXIGALVGVALMGAILHS 113 T++P +T V++ V A + R IGALVGVALMGAILHS Sbjct: 390 -------------TAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHS 436 Query: 114 FGGWITRIVLAYGVVAIVYALALVLVLRYV 143 GW R LA+GV+A YA AL LV R+V Sbjct: 437 VTGWNLRNPLAFGVMAAGYASALWLVWRFV 466 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.331 0.144 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 356,212 Number of extensions: 11801 Number of successful extensions: 141 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 4 Length of query: 159 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 81 Effective length of database: 1,250,540 Effective search space: 101293740 Effective search space used: 101293740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 127 (53.5 bits)