BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0254.0001.p
         (237 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313420|ref|YP_003777512.1| phenylacetyl-CoA-ligase [Herbas...   415   e-117
gi|300311876|ref|YP_003775968.1| phenylacetyl-CoA-ligase [Herbas...    72   6e-14

>gi|300313420|ref|YP_003777512.1| phenylacetyl-CoA-ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 446

 Score =  415 bits (1067), Expect = e-117,   Method: Compositional matrix adjust.
 Identities = 194/218 (88%), Positives = 208/218 (95%)

Query: 1   MPQRTPQPGDLEAIETASKDELQALQLQRLKWSLKHAYDNVPHYRASFDAAGVHPDDLKT 60
           M  R PQPG+LE IETAS+DELQALQLQRLKWSL+HAYDNVPHYR +FD AGVHPDDLK+
Sbjct: 1   MTIRIPQPGELEPIETASRDELQALQLQRLKWSLRHAYDNVPHYRNAFDQAGVHPDDLKS 60

Query: 61  LADLAKFPFTGKKDLRDNYPFNMFAVPREQVVRIHASSGTTGKPTVVGYTQNDIDTWATV 120
           L+DL++FPFT KKDLRD+YPF MFAVPREQVVR+HASSGTTGKPTVVGYTQNDIDTWATV
Sbjct: 61  LSDLSRFPFTTKKDLRDHYPFGMFAVPREQVVRVHASSGTTGKPTVVGYTQNDIDTWATV 120

Query: 121 VARSIRAAGGRKGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIQDF 180
           VARSIRAAGGR+GDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLI+DF
Sbjct: 121 VARSIRAAGGRRGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDF 180

Query: 181 KPSIIMVTPSYMLNIIEEFQRQGLDPVDSSLKVGIFGA 218
           KPSIIMVTPSYMLN+IEEFQRQG+DP  SSLKVGIFGA
Sbjct: 181 KPSIIMVTPSYMLNVIEEFQRQGMDPASSSLKVGIFGA 218


>gi|300311876|ref|YP_003775968.1| phenylacetyl-CoA-ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 423

 Score = 71.6 bits (174), Expect = 6e-14,   Method: Compositional matrix adjust.
 Identities = 53/159 (33%), Positives = 72/159 (45%), Gaps = 12/159 (7%)

Query: 52  GVHPDDLKTLADLAKFPFTGKKDLRD----NYPFN-MFAVPREQVVRIHASSGTTGKPTV 106
           GV    + + A LA+ P T K DL++      PF  + A P  Q+ R+  S G    P  
Sbjct: 49  GVDAAAISSRAALAQLPVTRKSDLKELQTRESPFGGLNATPTRQLRRLFVSPGPIFDPEG 108

Query: 107 VGYTQNDIDTWATVVARSIRAAGGRKGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMS 166
            G      D W    A  ++A G + G I+Q  + Y            A +LGC VIP  
Sbjct: 109 HGA-----DWWR--FASPMKALGLQAGHILQNCFAYHFTPAAFMVEGAAARLGCAVIPAG 161

Query: 167 GGQTEKQVQLIQDFKPSIIMVTPSYMLNIIEEFQRQGLD 205
            GQTE QV  +   KP   + TPS++  IIE+ Q  G D
Sbjct: 162 IGQTELQVNAMAALKPDAYVGTPSFLKIIIEKAQEMGAD 200


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.134    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 784,549
Number of extensions: 32751
Number of successful extensions: 90
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 89
Number of HSP's successfully gapped: 2
Length of query: 237
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 155
Effective length of database: 1,231,600
Effective search space: 190898000
Effective search space used: 190898000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 129 (54.3 bits)