BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0253.0006.p (476 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore... 660 0.0 gi|300312681|ref|YP_003776773.1| TonB-dependent siderophore rece... 191 1e-49 gi|300310762|ref|YP_003774854.1| outer membrane ferric sideropho... 157 2e-39 gi|300312551|ref|YP_003776643.1| ferrisiderophore receptor prote... 150 2e-37 gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore rece... 147 2e-36 >gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 704 Score = 660 bits (1702), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/480 (68%), Positives = 382/480 (79%), Gaps = 18/480 (3%) Query: 1 LLQADYIEDRRLTDFGIPSYQGRPVSVDPGKYYGAANARDADFSQSRVASTAATLTHRFN 60 LLQ DY+EDRR+ DFGIPSY+GRPV VDPG YGA NARDADF QSRVAST AT THRFN Sbjct: 239 LLQMDYLEDRRMMDFGIPSYRGRPVDVDPGTTYGARNARDADFVQSRVASTTATFTHRFN 298 Query: 61 DSLSLRNAFRYYDYHLNRNNTNISGNVNEVARTMSFGHAALSRDENGWFNQTELTQKLMI 120 D S RN RYYDYHLNRNNTN G VNE T + HA L RDENGWFNQ+ELTQKL + Sbjct: 299 DEFSFRNGLRYYDYHLNRNNTN-GGTVNEATATTTLNHAKLVRDENGWFNQSELTQKLQL 357 Query: 121 AGTRHEILYGVEVGQQSKDSVSSSGPTSGP-TSIFNPVLPVINPSAVTATNRTFGTYDTL 179 RHEILYG+E+G QSKD+ +SG + P SIFNPVLPV++P+ + T+GTYDT Sbjct: 358 GSIRHEILYGLELGSQSKDASQASGVVNFPGVSIFNPVLPVVDPNRLNPPTLTYGTYDTF 417 Query: 180 ASYVQDTIAFSDQWKALLGVRYDSFKQQSRVVTT---STANLSRTDNAFSPRAGLVWQPS 236 A+Y+QD+++ S+QWKAL+G+RYD ++QQSR++ STA+L RTD A SPRAGLVWQPS Sbjct: 418 ATYLQDSLSLSEQWKALVGLRYDRYRQQSRILNLANGSTADLQRTDTAVSPRAGLVWQPS 477 Query: 237 ETQSYYLSWSRSFQPSGETLPLATNNADLAPENTRNTEVGAKYDFLDGRASATVSVFRLE 296 TQSYYLSWSRSFQPSGE+ LATNNADLAPE TRNTE GAKYDF +GRASATVSVFRLE Sbjct: 478 LTQSYYLSWSRSFQPSGESFALATNNADLAPEQTRNTEAGAKYDFWEGRASATVSVFRLE 537 Query: 297 RDNIKAADPITRVIIPVGKQRTDGVELTLSADLKQGWKVLTGYAYLDAKVTDSIAVDGST 356 RDNIK+A P V+IPVGKQRTDG EL+ SADLKQGWK+L GYA+L+A +T+S + D Sbjct: 538 RDNIKSALP-NGVVIPVGKQRTDGAELSFSADLKQGWKLLAGYAWLNAVITESSSFD--- 593 Query: 357 NVAGSPTVANNVPIKGKRATLTAMHSANVWLTKEIGNGFTLGGGANAVGSRFANPGNTVS 416 N VP KGK ATLT HS N+WLT+E+G G +GGGANAVG+RFANPGNTV+ Sbjct: 594 ---------NGVPFKGKNATLTPRHSGNLWLTRELGEGLVVGGGANAVGARFANPGNTVT 644 Query: 417 LPGYFTADAMAAYKAANYSVQLNINNIFNTGYIVSGHGTSPNLSLPGAPRNVALTLRYSM 476 LPGY T DAMAAY+ + Y VQLNI N+FNTGYI+SGHGT+PNLS+PGAPRNVALTLRY M Sbjct: 645 LPGYVTVDAMAAYRTSKYEVQLNIGNLFNTGYIISGHGTNPNLSMPGAPRNVALTLRYHM 704 >gi|300312681|ref|YP_003776773.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 738 Score = 191 bits (485), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 154/500 (30%), Positives = 233/500 (46%), Gaps = 58/500 (11%) Query: 14 DFGIPSYQGRPVSVDPGKYYGAANARDADFSQSRVASTAATLTHRFNDSLSLRNAFRYYD 73 D+G+P G P +V YG ++D + VAS + H+ ++LRN ++ Sbjct: 260 DYGLPPLNGHPANVGRDTAYGL----NSDRTIQDVASFNVGVKHKIAPDVTLRNQTQFNY 315 Query: 74 YHLNRNNT--NISGNVNEVARTM-----------SFGHAALSRD----ENGWFNQTELTQ 116 H + T N G V+ T S S D + FNQTELT Sbjct: 316 VHTDAVETAPNTIGTVSGAGFTQLVNASSNLPLSSLFVRGQSHDRVITDYSIFNQTELTA 375 Query: 117 KLMIAGTRHEILYGVEVGQQSKDSV------SSSGPT---SGPTSIFNPVLPVINPSAVT 167 K +H+ + GVEVG D+ S +G +G T+ + +P++NPS Sbjct: 376 KFATGSIKHDAVVGVEVGHDGYDNQNYYRNGSCNGTALNANGATTGYVDCVPLLNPSYSA 435 Query: 168 AT--------NRTFGTYDTLASYVQDTIAFSDQWKALLGVRYDSFKQ---QSRVVTTSTA 216 A NR G+ +T+A+YV DT+ Q+K + G+RYD + S + A Sbjct: 436 AGSSVSSSTGNRAGGSANTVAAYVGDTVELVPQFKLVGGLRYDRYIASITNSIASARTPA 495 Query: 217 NLSRTDNAFSPRAGLVWQPSETQSYYLSWSRSFQPSGETLPLATNNADLAPENTRNTEVG 276 S+T N S R+G +WQP+ Q+YY+S+ SF PS E L +L PE R+ E+G Sbjct: 496 AASQTINFLSLRSGAIWQPTSAQAYYVSYGTSFNPSLEQLTGTQGQQNLDPEKNRSYELG 555 Query: 277 AKYDFLDGRASATVSVFRLERDNIKAADPITRVIIPVGKQRTDGVELTLSADLKQGWKVL 336 K+D DG A T + F++ +DN ++ T V GK R +G + + + W+V Sbjct: 556 GKWDLADGLA-LTAAAFQITKDNARS-QVATGVYALAGKVRVNGARAGATGHITREWQVA 613 Query: 337 TGYAYLDAKVTDSIAVDGSTNVAGSPTVANNVPIKGKRATLTAMHSANVWLTKEIGNGFT 396 Y YL AKV + A D S VP+ + TLT W T ++ + Sbjct: 614 ANYTYLGAKVLEGAAGDNSVG---------KVPVNTPKHTLT------TWTTYDLAPHWQ 658 Query: 397 LGGGANAVGSRFANPGNTVSLPGYFTADAMAAYKAANYSVQLNINNIFNTGYIVSGHGTS 456 +GGGA+ + R+A+ NTV + GY DAM AY Y ++LN+ N+ N Y + + Sbjct: 659 IGGGASYMSQRYADAPNTVQVGGYTRYDAMLAYTTKAYDIRLNLYNLSNKMYYDALIQSD 718 Query: 457 PNLSLPGAPRNVALTLRYSM 476 S+PG+ R L+L Y M Sbjct: 719 GGRSVPGSGRTALLSLTYRM 738 >gi|300310762|ref|YP_003774854.1| outer membrane ferric siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 717 Score = 157 bits (397), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 142/501 (28%), Positives = 212/501 (42%), Gaps = 59/501 (11%) Query: 1 LLQADYIEDRRLTDFGIP-----SYQGRPV-----SVDPGKYYGAANARDADFSQSRVAS 50 LLQA+Y E R+ D GIP + G P S +++GAA D + S Sbjct: 251 LLQAEYDEYERVPDRGIPGRAIATANGEPTAYALPSAGHEQFFGAAGR---DVIRDIHMS 307 Query: 51 TAATLTHRFNDSLSLRNAFRYYDYHLNRNNTNISGNVNEVARTMSFGHAALSRDENGWFN 110 +TLT + +LR+A D + N +NT ++ + +R + Sbjct: 308 LRSTLTRALSQDWTLRHALALMDLNSNFDNTFVTQAYQSA--PLDLDRVQRTRYQQN-LQ 364 Query: 111 QTELTQKLMIAGT--RHEILYGVEVGQQSKDSVSSSGPTSGPTSIFNPVLPVINPS---- 164 Q + L + G H++L GVE Q ++ S+ P + + P Sbjct: 365 QRSVQNNLELLGQLGAHDLLLGVEHSWQKREPRL--------WSVAAPAVSAVTPENTRN 416 Query: 165 ---AVTATNRTFGTYDTLASYVQDTIAFSDQWKALLGVRYDSFKQQSRVVTTSTANLSRT 221 T + L YVQD I + WK L G+R+D F+ SR + RT Sbjct: 417 GDDPTTPFQMNYHKASNLGLYVQDKITLAAHWKMLAGLRWDRFEVDSRNRRNGVRS-ERT 475 Query: 222 DNAFSPRAGLVWQPSETQSYYLSWSRSFQPSGETLPLATNNA-----DLAPENTRNTEVG 276 +A SPR G+VW+ Q YLSWS++F P G + T +A DL P+ +R E G Sbjct: 476 AHALSPRIGVVWEALPGQHAYLSWSKNFAPVGGDVIGITPDARGNFNDLKPQFSRQVEAG 535 Query: 277 AKYDFLDGRASATVSVFRLERDNIKAADPITRVIIP-VGKQRTDGVELTLSADLKQGWKV 335 K ++LD R S T++ F+LE N ADP+ G +R G+EL++ +L W V Sbjct: 536 IKSEWLDRRLSTTLAWFQLELFNRNVADPVRPGFFEQSGLERNRGIELSVEGELAAPWFV 595 Query: 336 LTGYAYLDAKVTDSIAVDGSTNVAGSPTVANNVPIKGKRATLTAMHSANVWLTKEIGNGF 395 G+A AK+ V P GKRA+ + +++L+ G+ Sbjct: 596 RAGWAIQSAKI-----------VQAEPKFV------GKRASGVSGRGGSLFLSYAPTLGW 638 Query: 396 TLGGGANAVGSRFANPGNTVSLPGYFTADAMAAYKAANYSVQLNINNIFNTGYIVSGHGT 455 G G+R+A+ N + LP Y D Y+ L + N+ N Y S G Sbjct: 639 FAELGLTVEGARYADRDNLLELPAYTRWDGKMGYRLDEAEFTLAVTNLANRRYYESATGL 698 Query: 456 SPNLSLPGAPRNVALTLRYSM 476 S PGAPR + LT Y Sbjct: 699 SQ--VRPGAPRALLLTAAYKF 717 >gi|300312551|ref|YP_003776643.1| ferrisiderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 755 Score = 150 bits (380), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 131/511 (25%), Positives = 230/511 (45%), Gaps = 61/511 (11%) Query: 2 LQADYIEDRRLTDFGIPSYQ----GRPV-----SVDPGKYYGAANARDADFSQSRVASTA 52 +Q D + D ++ G+P Y RP V +YG N D+++ T Sbjct: 260 IQQDNVPDGGVSTIGLPGYSTPDPNRPYISNAPKVSTSNFYGHVN----DYNRVNTDMTT 315 Query: 53 ATLTHRFNDSLSLRNAFRY----YDYHL----------NRNNTNISGNVNEVARTMSFGH 98 + H F+ L L+N RY DY L +R++ ++ E T Sbjct: 316 LRVEHDFSPDLKLQNTTRYARTDQDYRLSSFVAIGVKTDRSSVSLFTPSPEDRSTWEVSR 375 Query: 99 AALSRDENG---WFNQTELTQKLMIAGTRHEILYGVEVGQQSKDSVSSSGPTSGPTSIFN 155 ++R + NQT LT +H ++ G+E+ ++ + ++S +GP+ P + Sbjct: 376 NIITRRDQSNEILSNQTHLTASFQSGALQHTLVGGLELTRERQHALSFTGPSRRPRANLY 435 Query: 156 PVLPVINPSA--VTATNRTFGTYDTLASYVQDTIAFSDQWKALLGVRYDSFKQQ-SRVVT 212 P + S ++ R G DT++ Y DT+ F+++W LG+R D ++ Sbjct: 436 QPNPEFDDSGTLISVNGRNKGQTDTVSLYALDTLKFNERWSLSLGLREDFYRTSFDDTKP 495 Query: 213 TSTANLSRTDNAFSPRAGLVWQPSETQSYYLSWSRSFQPSGET---LPLATNNA---DLA 266 + L ++ F+ + +V++P+ S Y ++ + QP G L + +NA D A Sbjct: 496 NAGGPLHKSGGLFNAKVAVVFKPTSYSSLYALYASAQQPPGGANFNLSASRSNASHTDYA 555 Query: 267 PENTRNTEVGAKYDFLDGRASATVSVFRLERDNIKAADPITRVIIPVGKQRTDGVELTLS 326 P+ T E+G K++ LD + AT +++R E +N D +T GK+ T G+EL ++ Sbjct: 556 PQKTDTGEIGTKWELLDRKLIATAALYRTEVNNEVELDSLTNTYFQTGKKITQGLELGVT 615 Query: 327 ADLKQGWKVLTGYAYLDAKVTDSIAVDGSTNVAGSPTVANNVPIKGKRATLTAMHSANVW 386 DL + GY+++ ++V+ V + N R + T+ W Sbjct: 616 GDLSPTVSLTAGYSHMQSQVSRGALVSAT---------GENFLAYAPRRSFTS------W 660 Query: 387 LTKEIGNGFTLGGGANAVGSRF-ANPGNTVSLP----GYFTADAMAAYKAANYS-VQLNI 440 ++ + GFTLGGG G N + P Y+ AD MA+Y ++ +QLN+ Sbjct: 661 VSWRLPQGFTLGGGLRYSGGLLRGRDTNAIGTPKDTGAYWVADVMASYVIHQHAEIQLNV 720 Query: 441 NNIFNTGYIVSGHGTSPNLSLPGAPRNVALT 471 NN+FN Y+ S + + LPG RN++LT Sbjct: 721 NNLFNNSYVRSINKSGYRY-LPGDARNISLT 750 >gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 147 bits (370), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 138/485 (28%), Positives = 221/485 (45%), Gaps = 48/485 (9%) Query: 3 QADYIEDRRLTDFGIPSYQGRPVSVDPGKYYGAANARDADFSQSRVASTAATLTHRFNDS 62 + +++ + D GI + G + ++ G N D + TL R N++ Sbjct: 250 EGEFLRQKAPLDRGIVAVNGNAGVLPVSRFLGEPNDGDIKMQNQ---NHLLTLEQRLNEN 306 Query: 63 LSLRNAFRYYDYHLNRNNTNISGNVNEVARTMSFGHAALSRDEN----GWFN---QTELT 115 LR + + L+ +T A + +A L R W + Q E+ Sbjct: 307 WHLRTGIFHKEGSLDGYSTE--------AFALQADNATLRRQRRYRDYSWHDTSLQAEIG 358 Query: 116 QKLMIAGTRHEILYGVEVGQQSKDS----VSSSGPTSGPTSIFNPVLPVINPSAVTATNR 171 K AG HE+L G EV + D ++ S +I+NPV P+ T+ Sbjct: 359 GKFATAGVGHEVLVGTEVDWLTLDQRMLRINPSNVAPYAVNIYNPVYGQAQPTPTANTDT 418 Query: 172 TFGTYDTLASYVQDTIAFSDQWKALLGVRYDSFKQQSRVVTTSTANLSRTD-NAFSPRAG 230 + T Y+QD ++ S++W+ L GVR D+F+ S + ++R D +A+SPR G Sbjct: 419 SERQIST-GLYLQDQLSLSERWRLLAGVRADAFR--STLNNLRNGAVTRQDKHAYSPRLG 475 Query: 231 LVWQPSETQSYYLSWSRSFQP-SGETLPLATNNADLAPENTRNTEVGAKYDFLDGRASAT 289 L + + + S Y+S S+SF+P SG + + NA +PE +R E G K+ D R+ AT Sbjct: 476 LTYLLTPSWSVYVSASKSFRPNSGSDV---SGNA-FSPETSRAAEAGLKFQSEDKRSGAT 531 Query: 290 VSVFRLERDNIKAADPIT-RVIIPVGKQRTDGVELTLSADLKQGWKVLTGYAYLDAKVTD 348 ++VF + + N+ DP I G+ R+ GVEL +A L W+V AY DA V+ Sbjct: 532 LAVFDITKQNVLTTDPANPNFSIAAGEVRSKGVELDANARLGHHWRVTGNLAYTDAYVSR 591 Query: 349 SIAVDGSTNVAGSPTVANNVPIKGKRATLTAMHSANVWLTKEIGNGFTLGGGANAVGSRF 408 +T GS V N+P + + L A+ G + +GGG N VG R Sbjct: 592 D-----NTLPVGSRLV--NIP-RSSASLLAVFEDADA-----HGAPYGVGGGLNYVGRRT 638 Query: 409 ANPGNTVSLPGYFTADAMAAYK-AANYSVQLNINNIFNTGYIVSGHGTSPNLSLPGAPRN 467 + + LP Y TA A+A ++ + L++NN+FN Y S + + +PG RN Sbjct: 639 GDQAGSFMLPSYVTARALAYWQFTPKMKLSLDVNNLFNKRYYSSSYNSL--WIMPGDERN 696 Query: 468 VALTL 472 L L Sbjct: 697 ATLAL 701 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.129 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,435,526 Number of extensions: 56117 Number of successful extensions: 228 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 21 Length of query: 476 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 387 Effective length of database: 1,198,455 Effective search space: 463802085 Effective search space used: 463802085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)