BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0252.0003.p (166 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312400|ref|YP_003776492.1| hypothetical protein Hsero_3100... 259 6e-71 >gi|300312400|ref|YP_003776492.1| hypothetical protein Hsero_3100 [Herbaspirillum seropedicae SmR1] Length = 175 Score = 259 bits (663), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 129/173 (74%), Positives = 140/173 (80%), Gaps = 12/173 (6%) Query: 6 HIIKDKVVISDDWTVLRLNEGETPEGVAIPEGKIIVPLKVWQAQRATLQGHQELGVWFAS 65 IIK+K V+SDDWTVLRL EGET E V +P+G++IVPLKVWQAQ L+ E+GVW AS Sbjct: 3 EIIKNKTVVSDDWTVLRLAEGETAETVNVPQGRVIVPLKVWQAQGEALKSRAEIGVWLAS 62 Query: 66 DERPEALKDDLXS------------DGRGYSIAYNLRARLGYTGELRAIGDVLRDQLFYM 113 DERPE LK L S DGRGYSIAYNLRARLGYTGELRAIGDVLRDQLFYM Sbjct: 63 DERPEELKGQLDSFKVVAVDFPKFADGRGYSIAYNLRARLGYTGELRAIGDVLRDQLFYM 122 Query: 114 QRVGFNAFAVRADKDIHDALKGLTDFSEKYQTSWDEKNPLFRRAKRETVATRD 166 QRVGF+AFAVRADK+IHDA+KGLTDFSEKYQTSWDEKNPLFRR KRET T D Sbjct: 123 QRVGFDAFAVRADKNIHDAVKGLTDFSEKYQTSWDEKNPLFRRVKRETAQTAD 175 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 530,206 Number of extensions: 21375 Number of successful extensions: 45 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 44 Number of HSP's successfully gapped: 1 Length of query: 166 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 88 Effective length of database: 1,250,540 Effective search space: 110047520 Effective search space used: 110047520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)