BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0047.p (114 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis dom... 86 8e-19 gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis tra... 84 3e-18 gi|300311753|ref|YP_003775845.1| methyl-accepting chemotaxis tra... 68 2e-13 >gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 568 Score = 85.9 bits (211), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 50/127 (39%), Positives = 73/127 (57%), Gaps = 22/127 (17%) Query: 1 VLSLLMFGALQMILRAQHEIERAGDSRYESYKLANEVRSNSEHLTQSVRSYVVTGDPRYE 60 +++L++ AL + R + A +SRY S+ LA E+R +S+ LT+ R+YVVTGDP YE Sbjct: 25 LMTLIVLAALA-VDRYDARLNAANESRYASFLLATELRQSSDDLTRLARTYVVTGDPSYE 83 Query: 61 SIYWDIVAVSSGKKARP-------------DGRT--------VSMTDLMKQAGFTDQEFA 99 Y I+ + +GKKARP DG+ V++TDLM+ AGFT E Sbjct: 84 QQYEAILDIRNGKKARPEHYGRIYWDFVAADGKAPRPDSNQHVALTDLMRAAGFTAAEMG 143 Query: 100 KLNEAQS 106 KL EA++ Sbjct: 144 KLEEAKN 150 >gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 595 Score = 83.6 bits (205), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 53/133 (39%), Positives = 73/133 (54%), Gaps = 29/133 (21%) Query: 1 VLSLL------MFGALQMILRAQHEIERAGDSRYESYKLANEVRSNSEHLTQSVRSYVVT 54 VLS+L + GAL+ A E+ERA SRY+S LANE+R +E +T+ R+YV T Sbjct: 21 VLSILVAVVVSLTGALK---DANLELERAHQSRYDSTALANELRRTAEDMTKFARAYVTT 77 Query: 55 GDPRYESIYWDIVAVSSGKKARPD--------------------GRTVSMTDLMKQAGFT 94 GDP E Y+ I+ + +GK+ARP V + +LMK+AGFT Sbjct: 78 GDPNDEDRYYMIMDIRNGKRARPSNYDRFNWDLVNARKLALDPGAPGVPLGELMKRAGFT 137 Query: 95 DQEFAKLNEAQSL 107 D+E AK+ EA L Sbjct: 138 DEELAKMQEALKL 150 >gi|300311753|ref|YP_003775845.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 559 Score = 67.8 bits (164), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 39/112 (34%), Positives = 59/112 (52%), Gaps = 23/112 (20%) Query: 15 RAQHEIERAGDSRYESYKLANEVRSNSEHLTQSVRSYVVTGDPR---------------- 58 RA+ + +A ++R+ + LA E R + L Q RSYVV+G+PR Sbjct: 36 RARQALAQAQETRFTALALAAEFRQGVDDLAQLARSYVVSGEPRLLEQHQAVLDIRDGQR 95 Query: 59 -----YESIYWDIVAVSSGKKARPDGRT-VSMTDLMKQAGFTDQEFAKLNEA 104 Y +YWD+V + G+ RPD VS+ LM+QAG++ E A+L+EA Sbjct: 96 ARPQQYGRVYWDLVG-ADGQPPRPDSTVAVSLRKLMEQAGYSPAELARLDEA 146 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.127 0.351 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 321,038 Number of extensions: 10384 Number of successful extensions: 44 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 41 Number of HSP's successfully gapped: 3 Length of query: 114 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 41 Effective length of database: 1,274,215 Effective search space: 52242815 Effective search space used: 52242815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 124 (52.4 bits)