BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0250.0041.p
         (277 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313979|ref|YP_003778071.1| major facilitator superfamily p...   249   2e-67
gi|300310361|ref|YP_003774453.1| major facilitator superfamily p...    67   2e-12

>gi|300313979|ref|YP_003778071.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 400

 Score =  249 bits (636), Expect = 2e-67,   Method: Compositional matrix adjust.
 Identities = 136/208 (65%), Positives = 168/208 (80%)

Query: 1   MLGLLYASWAARIPSIRDNLQLDAATLSIVLLCGGLGAVGSFPLAAWLNAHYGPRRTTWY 60
           +LG+++++WAARIP+IRD LQLDAATL++VLLCGG+GAVGSFPLAAW+  HYG R ++ Y
Sbjct: 43  LLGVVFSTWAARIPAIRDQLQLDAATLALVLLCGGIGAVGSFPLAAWITGHYGARHSSLY 102

Query: 61  AGLVLLLTLPALGIAPNLALLMLFSAIYGAASSCFDVAINALGAVAEKQAGRSIMSLLHA 120
           AGLVLLL+LP L +AP L  LM    +YGAASSCFDVAINALGA AE++AGR IMS+LH 
Sbjct: 103 AGLVLLLSLPLLALAPGLWTLMAVCTLYGAASSCFDVAINALGAQAEREAGRPIMSMLHG 162

Query: 121 WFCVGTFSGALLGSAVAALEVTPVWHFLALGVLFLLPLRLGYKAMPNDRPEPSADKKIFA 180
           WFCVGTFSGAL+ SAVA L V P+WHFL    L   PL  G +A+P+DRP+    +++FA
Sbjct: 163 WFCVGTFSGALVASAVAGLGVAPLWHFLLAAALLAWPLYAGCQALPDDRPQYDPQRQLFA 222

Query: 181 IPHGHLVALGLIGFCGAIVEGSIAXTAG 208
           +PHGHLV LG+I FCGAIVEGS+A  +G
Sbjct: 223 LPHGHLVVLGVIAFCGAIVEGSVADWSG 250


>gi|300310361|ref|YP_003774453.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 390

 Score = 67.0 bits (162), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 61/207 (29%), Positives = 101/207 (48%), Gaps = 17/207 (8%)

Query: 3   GLLYASWAARIPSIRDNLQLDAATLSIVLLCGGLGAVGSFPLAAWLNAHYGPRRTTWYAG 62
           G  +A+W   IPS+++   L  A LS+ +L    GA+ +   +    A  G  R     G
Sbjct: 31  GATFATWGVLIPSVKERFALSDAVLSLAMLAVAGGALLTMGQSGKWIARVGSARALRQVG 90

Query: 63  LVLLLTLPALGIAPNLALLMLFSAIYGAASSCFDVAINALGAVAEKQAGRSIMSLLHAWF 122
           ++   TL  + +AP   +L+L   ++G+A + FDVA++   A+ E Q  R IMS LHA F
Sbjct: 91  ILYAATLLLIPLAPQFWMLVLLLLVFGSAMAAFDVAMSVQAALVEGQRTRPIMSTLHAMF 150

Query: 123 CVGTFSGALLGSAVAALEVTPVWHFLALGVLFL-LPLRLGYKAMPNDRPEPSADKKIFAI 181
              +  G    +  A   ++P+ H   +GV+ L + L +G  A+P++  + +A       
Sbjct: 151 ---SIGGIAGAALGAVPGLSPLAHAALVGVVTLAVSLSIGPFALPDEVHDSAAT------ 201

Query: 182 PHG-------HLVALGLIGFCGAIVEG 201
           PHG        ++ LG I F   + EG
Sbjct: 202 PHGADKRARRMVLVLGGIAFLALMCEG 228


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.325    0.141    0.444 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 755,183
Number of extensions: 25485
Number of successful extensions: 132
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 132
Number of HSP's successfully gapped: 2
Length of query: 277
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 193
Effective length of database: 1,222,130
Effective search space: 235871090
Effective search space used: 235871090
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 130 (54.7 bits)