BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0040.p (198 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313980|ref|YP_003778072.1| two component response regulato... 235 1e-63 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 117 7e-28 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 110 7e-26 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 107 7e-25 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 107 1e-24 >gi|300313980|ref|YP_003778072.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 199 Score = 235 bits (600), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 111/187 (59%), Positives = 141/187 (75%) Query: 7 PIQTIHTLQTQALDALLALQHLIDNYTPLHEDRVAALALQIGRRLELDAPRLQALHMAAR 66 P+ + + + +DA++A+ L D YTP HED+ A LA+ IGR L+L R+ ALHMA++ Sbjct: 2 PVSESNKIYQEVIDAMIAVHRLTDGYTPNHEDQTAELAVMIGRHLDLATERIDALHMASQ 61 Query: 67 VHDIGKINIPLDILNKPGKLSEAEFDLIKTHAQTSYEVLNRIDFSLPVADIVHAHHEYID 126 VHDIGK IPL +L KPG++S E+D++KTH Q YE+LN+I F +A+IVH HHEY+D Sbjct: 62 VHDIGKARIPLALLTKPGRISVEEYDVLKTHVQFGYEILNKISFPFNLAEIVHCHHEYLD 121 Query: 127 GSGYPRGLRGDDILLESRILTVADIVESMSEDRPYRKSLGMPAALQEISRLRGIRLDPAA 186 GSGYPRG+ GD+I LE+RILTVADIVESMS DRPYRKSLGM AAL EI R G+RLD Sbjct: 122 GSGYPRGISGDEIPLEARILTVADIVESMSADRPYRKSLGMSAALDEIQRQSGVRLDAVV 181 Query: 187 VDACLQL 193 VDACL + Sbjct: 182 VDACLDV 188 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 117 bits (293), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 61/154 (39%), Positives = 91/154 (59%), Gaps = 3/154 (1%) Query: 28 LIDNYTPLHEDRVAALALQIGRRLELDAPRLQALHMAARVHDIGKINIPLDILNKPGKLS 87 L D YT LH V AL + +GR L LD + + +HD+GK IP ++LNKPGKLS Sbjct: 154 LADEYTYLHSVAVCALMVALGRTLGLDETACREAGLGGFLHDVGKAFIPHELLNKPGKLS 213 Query: 88 EAEFDLIKTHAQTSYEVLNRIDFSLP--VADIVHAHHEYIDGSGYPRGLRGDDILLESRI 145 + EF IK H + Y+ L++ D ++P D+ HHE IDG+GYP GLR I L SR+ Sbjct: 214 DEEFSQIKKHPELGYKYLSQ-DPNVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRM 272 Query: 146 LTVADIVESMSEDRPYRKSLGMPAALQEISRLRG 179 + D+ ++++ DRPY+++ A+ ++ G Sbjct: 273 AAICDVYDALTSDRPYKRAWDPAEAISAMASWTG 306 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 110 bits (275), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 63/193 (32%), Positives = 109/193 (56%), Gaps = 4/193 (2%) Query: 3 DLADPIQTIHTLQTQALDALLALQHL--IDNYTPLHEDRVAALALQIGRRLELDAPRLQA 60 D A+ + I ++ DAL++L L D+YT LH V A+ + + +L + + + Sbjct: 130 DAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMMISLANQLGMTPQQTKQ 189 Query: 61 LHMAARVHDIGKINIPLDILNKPGKLSEAEFDLIKTHAQTSYEVLNRIDFSLPVA-DIVH 119 MA +HD+GK+ +PLDILNKP KL+E EF ++ H + +L +I+ VA D+ Sbjct: 190 AGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQIEGIGEVALDVSL 249 Query: 120 AHHEYIDGSGYPRGLRGDDILLESRILTVADIVESMSEDRPYRKSLGMPAALQEISRLRG 179 HHE IDGSGYP L+ D I + +++ V D+ ++++ +RPY+ +++ ++ G Sbjct: 250 HHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARSIRHMAASAG 309 Query: 180 IRLDPAAVDACLQ 192 DP + A ++ Sbjct: 310 -HFDPITMQAFVK 321 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 107 bits (267), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 60/183 (32%), Positives = 104/183 (56%), Gaps = 9/183 (4%) Query: 3 DLADPIQTIHTLQTQALDALLALQHL--IDNYTPLHEDRVAALALQIGRRLELDAPRLQA 60 + +D ++ I+ ++ DA+++L L DNYT +H V A+ + + +L L A Q Sbjct: 133 EASDLVREINASVSRNADAMISLVRLKTADNYTYMHSVAVCAMMISLADQLGLSAQERQQ 192 Query: 61 LHMAARVHDIGKINIPLDILNKPGKLSEAEFDLIKTHAQTSYEVLNRI----DFSLPVAD 116 MA +HDIGK+ +P ILNKP KL+E EF +++H + +L + D L D Sbjct: 193 AGMAGLLHDIGKMTVPPSILNKPSKLTEEEFVSVQSHPLAGHRLLQELPGIGDAPL---D 249 Query: 117 IVHAHHEYIDGSGYPRGLRGDDILLESRILTVADIVESMSEDRPYRKSLGMPAALQEISR 176 + HHE +DG+GYP L G DI + +R+ + D+ ++++ DRPY+ ++Q +++ Sbjct: 250 VSLHHHEKMDGTGYPYKLSGRDISMTARMGAICDVYDAITSDRPYKSGWDPSRSIQHMAQ 309 Query: 177 LRG 179 +G Sbjct: 310 SKG 312 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 107 bits (266), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 56/153 (36%), Positives = 85/153 (55%), Gaps = 1/153 (0%) Query: 28 LIDNYTPLHEDRVAALALQIGRRLELDAPRLQALHMAARVHDIGKINIPLDILNKPGKLS 87 L D YT LH V AL + +GR L L + ++ +HD+GK I +ILNKPGKL+ Sbjct: 151 LNDEYTYLHSVSVCALVIGLGRTLGLSEAACREAGLSGYLHDVGKAFIDDEILNKPGKLT 210 Query: 88 EAEFDLIKTHAQTSYEVLNRI-DFSLPVADIVHAHHEYIDGSGYPRGLRGDDILLESRIL 146 EF LIK H + Y+ L + D +D+ HHE +DG GYP L + I L +R+ Sbjct: 211 AEEFALIKRHPELGYQYLIQTPDMPAYASDVCRHHHERLDGRGYPDALPAESITLYARMT 270 Query: 147 TVADIVESMSEDRPYRKSLGMPAALQEISRLRG 179 V D+ ++++ +RPY+ A+ +++R G Sbjct: 271 AVCDVYDALTSERPYKHGWDPAEAISQMARWEG 303 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 574,782 Number of extensions: 22213 Number of successful extensions: 100 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 10 Length of query: 198 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 118 Effective length of database: 1,241,070 Effective search space: 146446260 Effective search space used: 146446260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)