BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0250.0039.p
         (371 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313981|ref|YP_003778073.1| major facilitator superfamily p...   307   1e-84

>gi|300313981|ref|YP_003778073.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 402

 Score =  307 bits (786), Expect = 1e-84,   Method: Compositional matrix adjust.
 Identities = 231/407 (56%), Positives = 266/407 (65%), Gaps = 47/407 (11%)

Query: 1   MPEPIAAAERDFIVRHPLLTALALSLGTAIALGVARFSYALLLSPMRTDLGWPYLIAGGM 60
           MP   A     FI++HP+LTALALSLGTA+ALGVARFSYALLL+PMR DLGWPYL+AGGM
Sbjct: 1   MPTDAARPPSPFILQHPILTALALSLGTAVALGVARFSYALLLAPMRADLGWPYLVAGGM 60

Query: 61  NTGNALGYLLGALITPPLMRRFGTHKMLIGGALATAFFIILSGLFIDTATLLALRIVTGA 120
           NTGNALGYLLGAL TP LMRRF  H++LI GA ATA    L  L  DT   L LR+VTG 
Sbjct: 61  NTGNALGYLLGALATPLLMRRFLAHRVLIAGAFATALLSFLPALTTDTLLQLLLRVVTGV 120

Query: 121 VSAFIFISGGILISRLAALHPRRAGFLLGLFYGGSGIGIVASAWLVPATMAAATAHGVAH 180
            SA IF+SGG+L+SRLAA+H  RAG LLGL+YGG+G+GIVA++ LVP  MAAA AHG AH
Sbjct: 121 SSALIFVSGGLLVSRLAAMHGHRAGLLLGLYYGGTGLGIVAASLLVPPVMAAALAHGAAH 180

Query: 181 SWQWAWLAMGLLSLVATAFMAPGPXPRPPALRIPALPPQTHGAGRFRMRTLSFGLTSYFM 240
            WQWAWL +G L+L A+  MA       PA  +P       G GR++ R+L FGL  Y  
Sbjct: 181 PWQWAWLLLGTLALAASLVMA------GPARLMPTTAAHVQGNGRWQARSLLFGLIGYLF 234

Query: 241 FGLGYIGYMTFVIALLKEQGMSASMITLFYTLLGLAVMASSRLWAGMLDKFKGGESLAIL 300
           FGLGYIGYMTFVIALLKE+GM A  ITLF+ LLG A  ASSR+WA MLD+F+GG+SLAIL
Sbjct: 235 FGLGYIGYMTFVIALLKEEGMPAERITLFFALLGAATFASSRIWARMLDRFRGGQSLAIL 294

Query: 301 N----------------------ALLSVATLLPAV---TAAVYHA-------TGVR---- 324
           N                       LL     L AV   TA V H         G+     
Sbjct: 295 NGLLALATLLPALTAEPVAVFASGLLFGGCFLSAVASTTAMVRHNLPPHHWPAGISAFTI 354

Query: 325 VRARXRCITPP-----ACACGGLQQGLLFSALALLIGALLAWRQRPL 366
           + A  + I P      A   GGLQ+GL FSAL LL GAL AWRQRPL
Sbjct: 355 IFAVGQIIGPTIVGWIADGAGGLQRGLSFSALMLLAGALTAWRQRPL 401


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.329    0.141    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 892,576
Number of extensions: 29826
Number of successful extensions: 238
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 236
Number of HSP's successfully gapped: 2
Length of query: 371
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 284
Effective length of database: 1,207,925
Effective search space: 343050700
Effective search space used: 343050700
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 131 (55.1 bits)