BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0036.p (380 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313983|ref|YP_003778075.1| histidinol-phosphate aminotrans... 514 e-147 gi|300313353|ref|YP_003777445.1| histidinol-phosphate aminotrans... 119 4e-28 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 70 2e-13 >gi|300313983|ref|YP_003778075.1| histidinol-phosphate aminotransferase 1 protein [Herbaspirillum seropedicae SmR1] Length = 360 Score = 514 bits (1323), Expect = e-147, Method: Compositional matrix adjust. Identities = 248/309 (80%), Positives = 279/309 (90%), Gaps = 3/309 (0%) Query: 1 MSKFWSPIVSRLTPYTPGEQPKISKLVKLNTNENPYGPSPKAIAAMAQEIGDNLRLYPNP 60 MSKFWSPIVSRLTPYTPGEQPKI++LVKLNTNE+PYGPSP A+ A+A+E+ D+LRLYPNP Sbjct: 1 MSKFWSPIVSRLTPYTPGEQPKIARLVKLNTNESPYGPSPLALQAIAREVSDSLRLYPNP 60 Query: 61 DAEPLKLAIAKRH---GLSPSQVFVGNGSDEVLAHAFQALLKHGAPILFPDITYSFYPTY 117 DAEPLK AIA+RH G+S +VFVGNGSDEVLAHAF ALL+HG PILFPDITYSFYPTY Sbjct: 61 DAEPLKQAIARRHAADGVSVREVFVGNGSDEVLAHAFHALLQHGKPILFPDITYSFYPTY 120 Query: 118 ASLYEVEYRAVPLAEDFTIRVDDYLGQGEAIGGIIFPNPNAPTGCLLALDEVERLLAGNP 177 A LY+VEYRAVPLAEDFT+R +DYLG G+AIGGIIFPNPNAPTGCLL L +VER+L GNP Sbjct: 121 AGLYQVEYRAVPLAEDFTLRSEDYLGHGQAIGGIIFPNPNAPTGCLLGLADVERILQGNP 180 Query: 178 DRVVIVDEAYIDFGGDSAIDLVTRHPNLLVVQTLSKSRSLAGLRVGFAVGHADLISALER 237 +RVV+VDEAYIDFGG SAI LV R+PNLLVVQTLSKSR+LAG+RVGFAVGHADLI ALER Sbjct: 181 ERVVVVDEAYIDFGGQSAIPLVQRYPNLLVVQTLSKSRALAGMRVGFAVGHADLIDALER 240 Query: 238 VKNSFNSYPLDRIAIAGATAAMEDDAHFDRTRNAVIASRSKLADELASLGFEVLPSAANF 297 VKNSFNSYPLDR AIAGATAA+ED+ +F +T N VIASR+ LA +LA LGF+VLPSAANF Sbjct: 241 VKNSFNSYPLDRPAIAGATAAIEDEDYFRKTCNMVIASRTALAAQLAGLGFQVLPSAANF 300 Query: 298 VFARHPQHD 306 +F RHPQHD Sbjct: 301 LFVRHPQHD 309 >gi|300313353|ref|YP_003777445.1| histidinol-phosphate aminotransferase 1 protein [Herbaspirillum seropedicae SmR1] Length = 372 Score = 119 bits (298), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 117/350 (33%), Positives = 169/350 (48%), Gaps = 38/350 (10%) Query: 8 IVSRLTPYTPGEQ----PKISKLVKLNTNENPYGPSPKAIAAMAQEIGD-NLRLYPNPDA 62 I + + P G Q P +VKL+ ENPY + +AQ + L YP P Sbjct: 17 IANVIRPVVRGWQSYHVPSAEGMVKLDAMENPYLLPAELREQLAQRLAALELNRYPVPSY 76 Query: 63 EPLKLAIAKRHGLSPS-QVFVGNGSDEVLAHAFQALLKHGAPILFPDITYSFYPTYASLY 121 LK AI K G+ V +GNGSDE++ A + G IL P+ + Y A + Sbjct: 77 TALKAAICKGLGVPAGYDVLLGNGSDELITLLSVACAQPGRTILAPEPGFVMYGVSAKMA 136 Query: 122 EVEYRAVPLAEDFTIRVDDYLGQGEAIGGII--FPNPNAPTGCLLALDEVERLL-AGNPD 178 +EY VPL D ++ + L EA +I PN PTG L + +V R++ A P Sbjct: 137 GLEYVGVPLCADLSLDLPAMLAAMEAHKPVITWLGYPNNPTGTLYEMADVLRIVEAAAPY 196 Query: 179 RVVIVDEAYIDFGGDSAIDLVTRHPNLLVVQTLSKSRSLAGLRVGFAVGHADLISALERV 238 +V+VDEAY F + + + +H NL++++T+SK LAG+R+G+ L+ L++V Sbjct: 197 GLVVVDEAYQPFAASTLMPALPQHNNLVLMRTVSK-LGLAGIRLGYLSAAPALLRELDKV 255 Query: 239 KNSFNSYPLDRIAIAGATAAMEDDAHFD-------RTRNAVIASRSKLADELASL-GFEV 290 + +N + + AA+ H D R R A RS LA ELA+L G +V Sbjct: 256 RPPYN------VNVLTEAAALFMLEHLDVLQAQAARLRQA----RSALAAELAALDGVQV 305 Query: 291 LPSAANFVFARHPQHDAAGLAASFXPRRLLVDLALQAGRA-----ECFRM 335 PSAANF+ R P DA + AS R+LV AGR C R+ Sbjct: 306 FPSAANFLLIRVP--DADKVCASLLAHRVLVK---NAGRMHVLLQNCLRI 350 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 70.5 bits (171), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 72/251 (28%), Positives = 120/251 (47%), Gaps = 37/251 (14%) Query: 78 SQVFVGNGSDEVLAHAFQALLKHGAPILFPDITYSFYPTYASLYEVEYRAVPLAE--DFT 135 S+ V GS E LAH A L G +L P+ +Y + A + R+V ++ DF Sbjct: 130 SEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSYPIHIYGAVIAGANIRSVRMSPGVDFF 189 Query: 136 IRVDDYLGQG-EAIGGIIFPNPNAPTGCLLALDEVERL--LAGNPDRVVIVDEAYIDFGG 192 ++ + + +I P+ PT + L+ ER+ LA +V+ D AY D Sbjct: 190 DELERAVRESYPKPKMMILGFPSNPTAQCVELEFFERVVKLAREHQILVVHDLAYADITF 249 Query: 193 DSAIDLVTRHPNLLVVQ----------TLSKSRSLAGLRVGFAVGHADLISALERVKNSF 242 D + P+++ V TLSKS ++AG R+GF VG+A L++AL R+K+ Sbjct: 250 DG-----WKAPSIMQVPGAREVAVEFFTLSKSYNMAGWRIGFMVGNARLVAALARIKSYH 304 Query: 243 NSYPLDRIAIAGATAAMEDD--------AHFDRTRNAVIASRSKLADELASLGFEV-LPS 293 + + +A A AA+E D A+++R RN ++ L G+ V +P Sbjct: 305 DYGSFTPVQVA-AIAALEGDQSCVEEIRANYERRRNVLVKG-------LHEAGWMVDVPK 356 Query: 294 AANFVFARHPQ 304 A+ +++AR P+ Sbjct: 357 ASMYIWARIPE 367 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,234,477 Number of extensions: 54218 Number of successful extensions: 192 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 4 Length of query: 380 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 293 Effective length of database: 1,207,925 Effective search space: 353922025 Effective search space used: 353922025 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)