BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0031.p (377 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 266 2e-72 gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 221 8e-59 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 168 6e-43 gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC t... 136 3e-33 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 100 3e-22 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 266 bits (681), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 135/352 (38%), Positives = 206/352 (58%), Gaps = 1/352 (0%) Query: 20 EEVQTVTIGFVGALSGVSESFGKGLANAAELAIFEANRNPFKINGKRVQFRLVRMDDRND 79 +E T+ IGF G LSG S GK + N +LA+ EANR +I GK Q RL+ DDR D Sbjct: 35 QETATLAIGFAGPLSGPSGGVGKSMQNGVQLALAEANRRGLRIGGKSWQLRLIAQDDRAD 94 Query: 80 AERARRVATALVQAEVVGVIGGTNSNTSIAVSKIFADAGIPQISPTSSFRRYTDQGFRTT 139 A VA +LVQ +V+ V+G S TS+A + ++ AG+ Q++P++ RR T + Sbjct: 95 PATAEFVARSLVQQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSAMSRRLTAMSYPRI 154 Query: 140 FRMLGIDDDACIYLGEYVVKELHAQRIAVIDNGTTFGVNVSSRFSEVAREHGGVIVSRDS 199 FR + ++ Y V +L + I ID+ T FG ++ +F+ A E G +V R S Sbjct: 155 FRTIPNNESLGRLAAGYAVDKLETRTIVTIDDRTPFGQGLAEQFARTASERGAQVVGRYS 214 Query: 200 VSDVLMNFGEILRRVKAQDIDAVFFGGYSEQTSMLAKSMLREEVKARLVTSMNGIVGSSF 259 VSD +F L + D +FFGG Q +LAKS+ R ++ ARL+ S G +G F Sbjct: 215 VSDKTSDFNAALLEARKLQPDLIFFGGLDWQAGVLAKSIRRLKLHARLMAS-PGTLGLPF 273 Query: 260 LISSGIAANGTVAQETGMPFNKMPGWKKFEADFSQRFDFQISIMTPYAYDAAHVLMAAVR 319 L+ +G ANG + E G P +KMPGWK F +S+ FD + + +AY+AA ++ +R Sbjct: 274 LMRAGPDANGALVLEPGPPQDKMPGWKSFRQRYSENFDSDMDLYAVFAYEAAQAIIQGIR 333 Query: 320 QANSLNPHKITNALHEISFKGLTGVIAFDAVGNPRSPSYTIYEVQNQKWKPM 371 QA S +P +I A+H + F+G++G +AF+ G+ PS+T+YEV++Q+W+P+ Sbjct: 334 QAGSADPERIAEAMHRLRFEGVSGPVAFNEEGDLLQPSFTMYEVKDQRWQPV 385 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 221 bits (563), Expect = 8e-59, Method: Compositional matrix adjust. Identities = 135/360 (37%), Positives = 198/360 (55%), Gaps = 12/360 (3%) Query: 8 SLALYASGAIAGEEVQTVTIGFVGALSGVSESFGKGLANAAELAIFEANRNPFKINGKRV 67 ++ L +GA +EV V I VG LSG + GK N A +A+ E N F I GK+V Sbjct: 13 AIGLAFAGAAYSQEV--VKIAHVGPLSGPNAHMGKDNENGARMAVDELNAKGFTIGGKKV 70 Query: 68 QFRLVRMDDRNDAERARRVATALVQAEVVGVIGGTNSNTSIAVSKIFADAGIPQISPTSS 127 +F LV DD +D ++A VAT LV +V VIG NS T+I SKI++DAGIPQ+SP+++ Sbjct: 71 KFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDAGIPQVSPSAT 130 Query: 128 FRRYTDQGFRTTFRMLGIDDDACIYLGEYVVKELHAQRIAVIDNGTTFGVNVSSRFSEVA 187 +YT QGF+T FR++ D LG+Y V++L ++IAVID+ T +G V+ F++ A Sbjct: 131 NPKYTQQGFKTAFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQGVADEFAKGA 190 Query: 188 REHGGVIVSRDSVSDVLMNFGEILRRVKAQDIDAVFFGGYSEQTSMLAKSMLREEVKARL 247 + GG IV +D +F IL +K + D VFFGG + + M + + A+ Sbjct: 191 KAAGGTIVGTQYTNDKATDFNAILTSLKGKKPDVVFFGGMDAVGGPMLRQMKQLGISAKF 250 Query: 248 VTSMNGIVGSSFLISSGIAANG------TVAQETGMPFNKMPGWKKFEADFSQRFDFQIS 301 + +GI S G+A +G A+ G+ G F A + ++F + Sbjct: 251 MGG-DGICTGSL---PGLAGDGLGDDQVICAEAGGVDEAGKKGMDDFRAAYKKKFGIDVV 306 Query: 302 IMTPYAYDAAHVLMAAVRQANSLNPHKITNALHEISFKGLTGVIAFDAVGNPRSPSYTIY 361 YAYDA + A+ +A S +P K L +IS KG+TGVIAFDA G+ + S T+Y Sbjct: 307 YNAAYAYDATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDIKDGSLTLY 366 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 168 bits (426), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 114/373 (30%), Positives = 186/373 (49%), Gaps = 4/373 (1%) Query: 2 LMLCVLSLALYASGAIAGEEVQTVTIGFVGALSGVSESFGKGLANAAELAIFEANRNPFK 61 L + SLAL + A+A +E Q V IGF L+G S GK +AI N P Sbjct: 8 LNIIAASLALIPAFAMA-QETQVVKIGFSSPLTGPQASAGKDNQGGLMMAIERLNAQPIT 66 Query: 62 INGKRVQFRLVRMDDRNDAERARRVATALVQAEVVGVIGGTNSNTSIAVSKIFADAGIPQ 121 + GK+++F ++ DD+ D + VA L V ++G NS +I S+++ DAGI Sbjct: 67 VGGKKIKFDVIAEDDQADPKSGVAVAQKLADQGVKAIVGPYNSGVTIPASRVYNDAGI-V 125 Query: 122 ISPTSSFRRYTDQGFRTTFRMLGIDDDACIYLGEYVVKELHAQRIAVIDNGTTFGVNVSS 181 ++ +S + T QGF T FR+ D + Y KEL +R+AVID+ T +G ++ Sbjct: 126 VATVASNPKITQQGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVIDDRTAYGQGLAQ 185 Query: 182 RFSEVAREHGGVIVSRDSVSDVLMNFGEILRRVKAQDIDAVFFGGYSEQTSMLAKSMLRE 241 F +VA+ +G +VS D +D +F IL +K + DAVF GGY+ Q + + M + Sbjct: 186 EFIKVAKANGIDVVSTDFTNDKATDFTAILTSIKGKKPDAVFLGGYAPQGGPIKRQMKQL 245 Query: 242 EVKARLVTSMNGIVGSSFLISSGIAANGTV-AQETGMPFNKMPGWKKFEADFSQRFDFQI 300 V L+ +GI G A +V + G +K K F ++ ++++ Sbjct: 246 GVDVPLMGG-DGICSPEMGRLGGDAIGESVYCTQGGTMLDKAKEGKVFSDEYQKKYNRPA 304 Query: 301 SIMTPYAYDAAHVLMAAVRQANSLNPHKITNALHEISFKGLTGVIAFDAVGNPRSPSYTI 360 YD ++ A++QANS++P + AL +IS+KG+ G FDA + + T+ Sbjct: 305 ETYAVSFYDGMMLIAQAMKQANSVDPKQFGPALAKISYKGVAGQYDFDANHDLKQSPVTV 364 Query: 361 YEVQNQKWKPMKT 373 Y ++ P+ + Sbjct: 365 YRFKDGLPVPLTS 377 >gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 136 bits (343), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 107/361 (29%), Positives = 168/361 (46%), Gaps = 24/361 (6%) Query: 20 EEVQTVTIGFVGALSGVSESFGKGLANAAELAIFEANRNPFKINGKR---VQFRLVRMDD 76 +E +T+ +GF G LS E + A+LAI EAN + KR ++F L+ DD Sbjct: 26 QERKTIALGFTGRLS---EPIAQSALQGAQLAIDEAN----ALAAKRRSPLRFELLPQDD 78 Query: 77 RNDAERARRVATALVQAEVVGVIGGTNSNTSIAVSKIFADAGIPQISPTSSFRRYTDQGF 136 + A +A V+ +V GVIG +S+ ++A + I+ A IPQI T++ ++T G Sbjct: 79 HGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIPQIGFTATSSQWTGMGN 138 Query: 137 RTTFRMLGIDDDACIYLGEYVVKELHAQRIAVIDNGTTFGVNVSSRFSEVAREHGG---- 192 R FR++G + L E + L +RI VI + + ++ E GG Sbjct: 139 RMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALADTLVE---NLGGSAQQ 195 Query: 193 VIVSRDSVSDVLMNFGEILRRVKAQDIDAVFFGGYSEQTSMLAKSMLREEVKARLVTSMN 252 + ++R V +F L+ +A D + F G Q R ARL+ + Sbjct: 196 IALARYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGANRTRTTARLLLTGG 255 Query: 253 GIVGSSFLISSGIAANGTVAQ-ETGMPFNKMPGWKKFEADFSQRFDFQISIMTPYAYDAA 311 S+ + + + V + E P P WK + +RF + + YAYDAA Sbjct: 256 A---SNLSLPAPLELRLHVLEYEIARP--HCPQWKVVTQTYQRRFGAAPTTYSFYAYDAA 310 Query: 312 HVLMAAVRQANSLNPHKITNALHEISFKGLTGVIAFDAVGNPRSPSYTIYEVQNQKWKPM 371 VL AA+ QA S N KI ALH + GL G ++FDA G P YT+ Q ++W+ + Sbjct: 311 SVLTAAIVQAGSDNGDKIATALHRMRHAGLHGTLSFDATGANAHPRYTLLRYQ-ERWQAV 369 Query: 372 K 372 + Sbjct: 370 E 370 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 99.8 bits (247), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 81/344 (23%), Positives = 154/344 (44%), Gaps = 14/344 (4%) Query: 23 QTVTIGFVGALSGVSESFGKGLANAAELAIFEANRNPFKINGKRVQFRLVRMDDRNDAER 82 +TV I G ++G +G + A L E NG + F V MDD + ++ Sbjct: 27 ETVKIAIAGPMTGAVAQYGD-MVKAGALTAIEQINAAGGANGNK--FEAVMMDDACEPKQ 83 Query: 83 ARRVATALVQAEVVGVIGGTNSNTSIAVSKIFADAGIPQISPTSSFRRYTD-QGFRTTFR 141 A VA +V + VIG S ++I S I+ + G+ I+P+++ + T+ + + FR Sbjct: 84 AVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFR 143 Query: 142 MLGIDDDACIYLGEYVVKELHAQRIAVIDNGTTFGVNVSSRFSEVAREHGGVIVSRDSVS 201 +G DD YV+++L +++AV+ + ++G V+S +V + ++ Sbjct: 144 TIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKAALDAAKVPVVVFEGIN 203 Query: 202 DVLMNFGEILRRVKAQDIDAVFFGGYSEQTSMLAKSMLREEVKARLVTSMNGIVGSSFLI 261 ++ I+ ++K+Q +D V+FGGY + ++ + + +KA + G+ Sbjct: 204 AGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKA-VFMGPEGVGNKDITA 262 Query: 262 SSGIAANGTV----AQETGMPFNKMPGWKKFEADFSQRFDFQISIMTPYAYDAAHVLMAA 317 +G A+ G + A P N A FQ+ AY ++ A Sbjct: 263 IAGPASEGMLVTLPADFAADPANAALVKAFAAAKRDPNGPFQMP-----AYSGVKIIADA 317 Query: 318 VRQANSLNPHKITNALHEISFKGLTGVIAFDAVGNPRSPSYTIY 361 + A S +P K+ +H SFK G + +D G+ +S + ++ Sbjct: 318 IAGAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGDLKSFKFVVF 361 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,077,928 Number of extensions: 42756 Number of successful extensions: 180 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 8 Length of query: 377 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 290 Effective length of database: 1,207,925 Effective search space: 350298250 Effective search space used: 350298250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)