BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0029.p (433 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313989|ref|YP_003778081.1| ABC transporter ATPase [Herbasp... 699 0.0 gi|300310722|ref|YP_003774814.1| ABC transporter ATPase [Herbasp... 224 9e-60 gi|300311530|ref|YP_003775622.1| ABC transporter ATPase [Herbasp... 146 4e-36 gi|300311473|ref|YP_003775565.1| ABC transporter ATPase [Herbasp... 139 4e-34 gi|300311132|ref|YP_003775224.1| ABC-type bacteriocin/lantibioti... 75 1e-14 >gi|300313989|ref|YP_003778081.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 555 Score = 699 bits (1804), Expect = 0.0, Method: Compositional matrix adjust. Identities = 342/363 (94%), Positives = 350/363 (96%) Query: 1 MNRVGKIVPPKRQILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEGDATPAP 60 MNRVGKIVPPKRQILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEG+A P P Sbjct: 8 MNRVGKIVPPKRQILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEGEAIPMP 67 Query: 61 NLNIGYLPQEPQLDPEKNVRQEVESALGEVFEAQAKLDVVYAAYAEEDADFDALATEQAR 120 NLNIGYLPQEPQLD EK VRQEVESALGEVFEAQAKLD VYAAYAEEDADFDALATEQAR Sbjct: 68 NLNIGYLPQEPQLDAEKTVRQEVESALGEVFEAQAKLDAVYAAYAEEDADFDALATEQAR 127 Query: 121 LEAIISASDGNSLNQQLEMAADALRLPPWDAKIAVLSGGEKRRVALCKLLLSKPDMLLLD 180 LEAII+A+DGNSLNQQLEMAADALRLPPWDAKI VLSGGEKRRVALCKLLLSKPDMLLLD Sbjct: 128 LEAIIAAADGNSLNQQLEMAADALRLPPWDAKIGVLSGGEKRRVALCKLLLSKPDMLLLD 187 Query: 181 EPTNHLDAESVDWLEQFLLRFPGTVVAITHDRYFLDNAAEWILELDRGHGIPWKGNYSSW 240 EPTNHLDAESVDWLEQFL RFPGTVVAITHDRYFLDNAAEWILELDRGHGIPWKGNYSSW Sbjct: 188 EPTNHLDAESVDWLEQFLQRFPGTVVAITHDRYFLDNAAEWILELDRGHGIPWKGNYSSW 247 Query: 241 LEQKEARLKQEEATESARQKTIAKELEWVRQNPKGRQAKSKARLSRFNELSEHEYQKRNE 300 LEQKEARLKQEEA+ESARQKTIAKELEWVRQNPKGRQAKSKARL+RFNELSE+EYQKRNE Sbjct: 248 LEQKEARLKQEEASESARQKTIAKELEWVRQNPKGRQAKSKARLARFNELSEYEYQKRNE 307 Query: 301 TSEIFIPVAERLGNEVIEFKGVSKGYGDRLLIDNLNFKIPAGAIVGIIGPNGAGKTTHXR 360 T EIFIPVAERLGNEVIEFK VSKGYGDRLLID+LNFKIPAGAIVGIIGPNGAGK+T R Sbjct: 308 TQEIFIPVAERLGNEVIEFKNVSKGYGDRLLIDDLNFKIPAGAIVGIIGPNGAGKSTLFR 367 Query: 361 RRA 363 A Sbjct: 368 MLA 370 Score = 102 bits (253), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 72/244 (29%), Positives = 117/244 (47%), Gaps = 38/244 (15%) Query: 12 RQILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEGDATPAPNLNIGYLPQEP 71 R ++ D++ GA +G++G NG+GKSTL +++AG + G+ P + + + Q Sbjct: 336 RLLIDDLNFKIPAGAIVGIIGPNGAGKSTLFRMLAGRETPDSGEVVLGPTVKVSLVDQSR 395 Query: 72 Q-LDPEKNVRQEVESALGEVFEAQAKLDVVYAAYAEEDADFDALATEQARLEAIISASDG 130 + L +K V ++V G + ++ AY F+ +Q ++ Sbjct: 396 EDLGNKKTVFEDVSG--GADILTVGRFEMPSRAYL---GRFNFKGGDQQKI--------- 441 Query: 131 NSLNQQLEMAADALRLPPWDAKIAVLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAES 190 + LSGGE+ R+ L K LL ++LLLDEP+N LD E+ Sbjct: 442 ----------------------VGNLSGGERGRLHLAKTLLQGGNVLLLDEPSNDLDVET 479 Query: 191 VDWLEQFLLRFPGTVVAITHDRYFLDNAAEWILELDRGHGIP-WKGNYSSWLEQKEARLK 249 + LE LL F GTV+ I+HDR+FLD A I+ + + + GNY + K+ RL Sbjct: 480 LRALEDALLEFAGTVLVISHDRWFLDRIATHIIAFEGDSQVTFFDGNYQEYEADKKKRLG 539 Query: 250 QEEA 253 +E A Sbjct: 540 EEAA 543 >gi|300310722|ref|YP_003774814.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 640 Score = 224 bits (572), Expect = 9e-60, Method: Compositional matrix adjust. Identities = 130/346 (37%), Positives = 196/346 (56%), Gaps = 5/346 (1%) Query: 14 ILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEGDATPAPNLNIGYLPQEPQL 73 +L S G +IG++G NG+GKS+LLKI+AG K +G NL I Y+ QEP Sbjct: 18 LLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLLVMQQNLKIAYVDQEPHF 77 Query: 74 DPEKNVRQEVESALGEVFEAQAKLDVVYAAYAEEDADFDALATEQARLEAIISASDGNSL 133 E V V S LGE+ A+ + + + ++ D AL +++ ++ A+D S+ Sbjct: 78 PEEMTVFDAVASGLGELPALLAEYEAITGQFGGDNDD--ALLERMHQIQTVLDAADAWSI 135 Query: 134 NQQLEMAADALRLPPWDAKIAVLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVDW 193 ++E D L L DA+I LSGG K+RVAL L+ PD+LLLDEPTNHLD S+ W Sbjct: 136 GNKVETTLDKLNLNR-DARIGELSGGMKKRVALACGLVGNPDVLLLDEPTNHLDFGSILW 194 Query: 194 LEQFLLRFPGTVVAITHDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEARLKQEEA 253 LE L F G+++ ITHDR FLDN + I+ELDRG + + GN++++ +K L EE Sbjct: 195 LEGLLRDFKGSILFITHDRSFLDNVSTRIIELDRGKILSFPGNFTTYQTRKAELLANEEV 254 Query: 254 TESARQKTIAKELEWVRQNPKGRQAKSKARLSRFNELSEHEYQKRNETSEIFIPV--AER 311 + K +A+E W+R+ + R+ + + R+ R L ++R ++ + V ER Sbjct: 255 ENAKFDKFLAQEEVWIRKGVQARRTRDEGRVRRLEALRVQRSERRERQGQVKLEVGTGER 314 Query: 312 LGNEVIEFKGVSKGYGDRLLIDNLNFKIPAGAIVGIIGPNGAGKTT 357 G V E + V+K YG + ++ + + + G VG+IG NGAGKTT Sbjct: 315 SGKIVAELENVNKAYGPKTIVRDFSTILMRGDKVGLIGQNGAGKTT 360 Score = 89.4 bits (220), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 73/248 (29%), Positives = 114/248 (45%), Gaps = 43/248 (17%) Query: 1 MNRVGKIVPPKRQILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEGDATPAP 60 + V K PK I++D S G K+G++G NG+GK+TLLK++ G + G Sbjct: 322 LENVNKAYGPK-TIVRDFSTILMRGDKVGLIGQNGAGKTTLLKLILGEEAPDSGTVKQGT 380 Query: 61 NLNIGYLPQ-EPQLDPEKNVRQEVESALGEV-FEAQAKLDVVYAAYAEEDADFDALATEQ 118 + + Y Q QL+ E + + V Q K + Y + DF A E+ Sbjct: 381 KMQVAYFDQMRAQLNEETTLADTIAPGSDWVEVNGQRKHVMTYLS------DF-LFAPER 433 Query: 119 ARLEAIISASDGNSLNQQLEMAADALRLPPWDAKIAVLSGGEKRRVALCKLLLSKPDMLL 178 AR + + LSGGE+ R+ L +L ++L+ Sbjct: 434 AR------------------------------SPVRTLSGGERNRLLLARLFAKPANVLV 463 Query: 179 LDEPTNHLDAESVDWLEQFLLRFPGTVVAITHDRYFLDNAAEWILELDRGHGI--PWKGN 236 LDEPTN LD ++++ LE+ L + GTV ++HDR FLDN ++ G+G+ + G Sbjct: 464 LDEPTNDLDIDTLELLEELLEEYEGTVFLVSHDRMFLDNVVTQVIA-SEGNGVWREYVGG 522 Query: 237 YSSWLEQK 244 YS W Q+ Sbjct: 523 YSDWERQR 530 >gi|300311530|ref|YP_003775622.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 146 bits (368), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 110/355 (30%), Positives = 186/355 (52%), Gaps = 16/355 (4%) Query: 14 ILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEGDATPAPNLNIGYLPQEPQL 73 + ++IS+ F G + G++G NG GKST +KI+ G + G+ + N +G L Q+ Sbjct: 16 LFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDVNERLGKLRQDQFA 75 Query: 74 DPEKNVRQEVESALGEVFEAQAKLDVVYAAYAEEDADFDALATEQARLEAIISASDGNSL 133 E V V E++ A ++ D +YA D D+ + A LEA + DG + Sbjct: 76 YEEMRVLDVVMMGHVEMWAAMSERDAIYANPDATDDDY----MKAADLEAKFAEYDGYTA 131 Query: 134 NQQLEMAADALRLP--PWDAKIAVLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESV 191 + + +P + ++ ++ G K RV L + L S PD+LLLDEPTN+LD ++ Sbjct: 132 EARAGELLLGVGVPIEQHNGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDINTI 191 Query: 192 DWLEQFLLRFPGTVVAITHDRYFLDNAAEWILELDRGHGIPWKGNYSSW-LEQKEARLKQ 250 WLE+ L T++ I+HDR+FL+ + ++D G + GNY + L +AR +Q Sbjct: 192 RWLEEVLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKIYPGNYDDYMLASSQARAQQ 251 Query: 251 EEATESARQKTIAKELEWVRQ----NPKGRQAKSKARLSRFNELSEHEYQKRNETSE-IF 305 A A++K +A+ ++VR+ K RQA S+A+ + +++ + + + + I Sbjct: 252 LAANAKAKEK-VAELQDFVRRFSANKSKARQATSRAK--QIDKIKVEDIKPSSRVNPFIR 308 Query: 306 IPVAERLGNEVIEFKGVSKGYGDRLLIDNLNFKIPAGAIVGIIGPNGAGKTTHXR 360 ++L +E +G++K Y DR + N + + AG + IIG NGAGKTT R Sbjct: 309 FEGEKKLHRLAVETQGITKTY-DRDIFKNFSIMVEAGEKIAIIGANGAGKTTLLR 362 Score = 111 bits (277), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 74/233 (31%), Positives = 112/233 (48%), Gaps = 40/233 (17%) Query: 12 RQILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEGDATP---APNLNIGYLP 68 R I K+ S+ G KI ++G NG+GK+TLL+ + G + D A N N+GY+P Sbjct: 331 RDIFKNFSIMVEAGEKIAIIGANGAGKTTLLRSIGGDVTGLHPDGGLVKWAENANVGYMP 390 Query: 69 QEPQLDPEKNVRQEVESALGEVFEAQAKLDVVYAAYAEEDADFDALATEQARLEAIISAS 128 Q+P E F L + +E D A+ RL + S Sbjct: 391 QDPT----------------EEFAGGETLTDWMGKWTQEGDDDQAVRGILGRL--LFSGD 432 Query: 129 DGNSLNQQLEMAADALRLPPWDAKIAVLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDA 188 D + VLSGGEK R+ KL+L + ++LL+DEPTNH+D Sbjct: 433 D-------------------VKKSVKVLSGGEKGRMMYGKLMLGRHNVLLMDEPTNHMDM 473 Query: 189 ESVDWLEQFLLRFPGTVVAITHDRYFLDNAAEWILELDRGHGIPWKGNYSSWL 241 ES++ L L ++ GT++ ++HDR F+ + A ILE+ G I ++G Y +L Sbjct: 474 ESIESLNIALEKYAGTLIFVSHDREFVSSLATRILEVKDGQVIDFQGGYEDYL 526 >gi|300311473|ref|YP_003775565.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 139 bits (351), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 107/365 (29%), Positives = 184/365 (50%), Gaps = 34/365 (9%) Query: 14 ILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEGDATPAPNLNIGYLPQE-PQ 72 +L+++ L+ PG KIG++G NG+GKS+L ++ G +G+ + Y+ QE P Sbjct: 16 LLENVDLTLNPGDKIGLIGANGAGKSSLFAMLRGELHADQGNIDFPARWRMAYVAQETPA 75 Query: 73 LDPEKNVRQEVESAL-GEVFEAQAKLDVVYAAYAEEDADFDALATEQARLEAIISASDGN 131 LD R +E A+ G+ Q + ++ A AE D A L ++ +D Sbjct: 76 LD-----RPAIEYAIDGDAHLRQLEAEL---ARAEAQPDDKHDGNHIAELHTALADADAY 127 Query: 132 SLNQQLE--MAADALRLPPWDAKIAVLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAE 189 ++ + E + L + +A SGG + R+ L + L+ D+LLLDEPTNHLD + Sbjct: 128 TVRSRAEQLLLGLGFSLEQMERPVASFSGGWRMRLNLAQALMCPSDLLLLDEPTNHLDLD 187 Query: 190 SVDWLEQFLLRFPGTVVAITHDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEARLK 249 ++ WLE +L R+ GT++ I+HDR FLD I+ +D + GNYS++ Q+ A+L+ Sbjct: 188 AIIWLEDWLKRYEGTLLIISHDRDFLDGVVNVIVHIDDRKLKRYSGNYSAFERQRSAQLE 247 Query: 250 QEEATESARQKTIAKELEWVR----QNPKGRQAKSKAR-LSRFNELSEHEYQKRNETSEI 304 +Q+ A ++ + K RQA+S+ + LS+ EL+ +E Sbjct: 248 LMAGMIEKQQRQRAHLQSFIDRFKAKATKARQAQSRMKALSKMEELA-----PLRAAAEF 302 Query: 305 FIPVAERLG--NEVIEFKGVSKGYG----------DRLLIDNLNFKIPAGAIVGIIGPNG 352 E L N ++ + V+ GY D++++ ++F + G +G++G NG Sbjct: 303 SFEFREPLSAPNPLLVMEKVNAGYHIEGKNGAPDEDKIIVHGIDFSLQNGQRIGLLGVNG 362 Query: 353 AGKTT 357 AGK+T Sbjct: 363 AGKST 367 Score = 78.6 bits (192), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 40/94 (42%), Positives = 58/94 (61%), Gaps = 3/94 (3%) Query: 151 AKIAVLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVDWLEQFLLRFPGTVVAITH 210 + IA SGGEK R+AL ++ +P++LLLDEPTNHLD E+ + L L +F GT+V ++H Sbjct: 440 SSIAPFSGGEKARLALALIVWQRPNLLLLDEPTNHLDLETREALTMALAQFEGTLVLVSH 499 Query: 211 DRYFLDNAAEWILELDRGHGIPWKG---NYSSWL 241 DR+ L + L + G P+ G +Y WL Sbjct: 500 DRHLLRATTDQFLIVAEGKLQPFDGDLDDYKDWL 533 >gi|300311132|ref|YP_003775224.1| ABC-type bacteriocin/lantibiotic ATPase/permease fusion exporter protein [Herbaspirillum seropedicae SmR1] Length = 567 Score = 74.7 bits (182), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 72/230 (31%), Positives = 113/230 (49%), Gaps = 53/230 (23%) Query: 15 LKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKE------IEGDATPAPNLN----- 63 L++ISL+ PG K+G++G +GSGKSTLL+ +AG+ + I+G A A Sbjct: 362 LRNISLTIQPGEKVGIVGRSGSGKSTLLRTLAGLHQAEEGHVLIDGVAVTAYAEEVRMRC 421 Query: 64 IGYLPQEPQLDPEKNVRQEV---ESALGEVFEAQAKLDVVYAAYAEEDADFDALATEQAR 120 IG+ PQEP L + V + + G+V+E AA A D D +A + R Sbjct: 422 IGFKPQEPFL-FDGTVAANIFVGDRVPGQVYE---------AALAVSSLD-DLIARGELR 470 Query: 121 LEAIISASDGNSLNQQLEMAADALRLPPWDAKIAVLSGGEKRRVALCKLLLSKPDMLLLD 180 L+ +I A GN LSGG+++ VAL +++ P +LLLD Sbjct: 471 LDQLIKAP-GN------------------------LSGGQRQMVALARVIACTPRILLLD 505 Query: 181 EPTNHLDAESVDWLEQFLLRFPG--TVVAITHDRYFLDNAAEWILELDRG 228 EPT +D ++ + + L+ + G T+V TH L + + I+ +D G Sbjct: 506 EPTTGIDQQTEARIIERLVGYAGGRTLVVATHSPALLRH-MDRIVVIDGG 554 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,131,276 Number of extensions: 40530 Number of successful extensions: 518 Number of sequences better than 1.0e-08: 23 Number of HSP's gapped: 514 Number of HSP's successfully gapped: 27 Length of query: 433 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 345 Effective length of database: 1,203,190 Effective search space: 415100550 Effective search space used: 415100550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)