BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0250.0026.p
         (134 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310745|ref|YP_003774837.1| amidase family protein [Herbasp...    54   5e-09
gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp...    53   8e-09

>gi|300310745|ref|YP_003774837.1| amidase family protein
           [Herbaspirillum seropedicae SmR1]
          Length = 611

 Score = 53.9 bits (128), Expect = 5e-09,   Method: Compositional matrix adjust.
 Identities = 37/102 (36%), Positives = 47/102 (46%), Gaps = 3/102 (2%)

Query: 15  SSMPVDASASSAAHCFVPYPAPAVTAISSGPLSGLRFAVKDIFDVAGYPTGCGNPHMLAL 74
           S +P       AA   + +     T + S PL G+ FAVKD  DVAG PT    P     
Sbjct: 52  SRVPAKQLKQQAAALDLMWQIKGETVLESMPLFGIPFAVKDNIDVAGMPTTAACPE---F 108

Query: 75  SGIKTASAPSVTALAQAGATFVGKTYTDELAFSMNGKNAHFG 116
           S +   SA  V  L  AGA  VGKT  D+ A  + G  + +G
Sbjct: 109 SRVAETSATVVQRLQAAGALLVGKTNLDQFATGLVGVRSPYG 150


>gi|300311162|ref|YP_003775254.1| amidase family protein
           [Herbaspirillum seropedicae SmR1]
          Length = 450

 Score = 53.1 bits (126), Expect = 8e-09,   Method: Compositional matrix adjust.
 Identities = 33/75 (44%), Positives = 44/75 (58%), Gaps = 5/75 (6%)

Query: 45  PLSGLRFAVKDIFDVAGYPTGCGNPHMLALSG--IKTASAPSVTALAQAGATFVGKTYTD 102
           PL G+  +VKD+FDVAG  T  G+   + L G  + +A A  V  L +AGA  +GKT   
Sbjct: 68  PLEGVPVSVKDLFDVAGETTLAGS---VVLRGKPVASAHAAVVQNLLKAGAILIGKTNMT 124

Query: 103 ELAFSMNGKNAHFGT 117
           E A+S  G N H+GT
Sbjct: 125 EFAYSGLGINPHYGT 139


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.315    0.129    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 427,870
Number of extensions: 17151
Number of successful extensions: 79
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 83
Number of HSP's successfully gapped: 2
Length of query: 134
Length of database: 1,619,870
Length adjustment: 76
Effective length of query: 58
Effective length of database: 1,260,010
Effective search space: 73080580
Effective search space used: 73080580
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 125 (52.8 bits)